PMID- 29241586
OWN - NLM
STAT- MEDLINE
DCOM- 20190219
LR  - 20190320
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 324
DP  - 2018 Feb
TI  - Influence of membrane cholesterol on monocyte chemotaxis.
PG  - 74-77
LID - S0008-8749(17)30224-1 [pii]
LID - 10.1016/j.cellimm.2017.12.005 [doi]
AB  - Cholesterol content influences several important physiological functions due to 
      its effect on membrane receptors. In this work, we tested the hypothesis that 
      cellular cholesterol alters chemotactic response of monocytes to Monocyte 
      Chemoattractant Protein-1 (MCP-1) due to their effect on the receptor, CCR2. We 
      used Methyl-beta-cyclodextrin (MbetaCD) to alter the baseline cholesterol in human 
      monocytic cell line THP-1, and evaluated their chemotactic response to MCP-1. 
      Compared to untreated cells, cholesterol enrichment increased the number of 
      monocytes transmigrated in response to MCP-1 while depletion had opposite effect. 
      Using imaging flow cytometry, we established that these differences were due to 
      alterations in expression levels, but not the surface distribution, of CCR2.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Saha, Amit K
AU  - Saha AK
AD  - Department of Biomedical, Chemical and Materials Engineering, San Jose State 
      University, San Jose, CA 95192, USA.
FAU - Mousavi, Marzieh
AU  - Mousavi M
AD  - Department of Biomedical, Chemical and Materials Engineering, San Jose State 
      University, San Jose, CA 95192, USA.
FAU - Dallo, Shatha F
AU  - Dallo SF
AD  - Department of Biomedical Engineering, The University of Texas at San Antonio, San 
      Antonio, TX 78249, USA.
FAU - Evani, Shankar J
AU  - Evani SJ
AD  - Department of Biomedical Engineering, The University of Texas at San Antonio, San 
      Antonio, TX 78249, USA.
FAU - Ramasubramanian, Anand K
AU  - Ramasubramanian AK
AD  - Department of Biomedical, Chemical and Materials Engineering, San Jose State 
      University, San Jose, CA 95192, USA. Electronic address: 
      anand.ramasubramanian@sjsu.edu.
LA  - eng
GR  - G12 MD007591/MD/NIMHD NIH HHS/United States
GR  - SC1 HL112629/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20171209
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (beta-Cyclodextrins)
RN  - 0 (methyl-beta-cyclodextrin)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Chemotaxis, Leukocyte/*physiology
MH  - Cholesterol/*metabolism
MH  - Humans
MH  - Monocytes/*metabolism
MH  - Receptors, CCR2/*metabolism
MH  - Receptors, Chemokine/*metabolism
MH  - THP-1 Cells
MH  - beta-Cyclodextrins/pharmacology
PMC - PMC5808849
MID - NIHMS927230
OTO - NOTNLM
OT  - AMNIS
OT  - CCR2
OT  - Cholesterol
OT  - MCP-1
OT  - MbetaCD
OT  - THP-1
OT  - Transwell
EDAT- 2017/12/16 06:00
MHDA- 2019/03/21 06:00
PMCR- 2019/02/01
CRDT- 2017/12/16 06:00
PHST- 2017/07/05 00:00 [received]
PHST- 2017/10/13 00:00 [revised]
PHST- 2017/12/08 00:00 [accepted]
PHST- 2017/12/16 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PHST- 2017/12/16 06:00 [entrez]
PHST- 2019/02/01 00:00 [pmc-release]
AID - S0008-8749(17)30224-1 [pii]
AID - 10.1016/j.cellimm.2017.12.005 [doi]
PST - ppublish
SO  - Cell Immunol. 2018 Feb;324:74-77. doi: 10.1016/j.cellimm.2017.12.005. Epub 2017 
      Dec 9.