PMID- 29241918
OWN - NLM
STAT- MEDLINE
DCOM- 20180801
LR  - 20181202
IS  - 1875-6263 (Electronic)
IS  - 1028-4559 (Linking)
VI  - 56
IP  - 6
DP  - 2017 Dec
TI  - Human amniotic fluid stem cells have better potential in early second trimester 
      of pregnancy and can be reprogramed to iPS.
PG  - 770-774
LID - S1028-4559(17)30251-6 [pii]
LID - 10.1016/j.tjog.2017.10.012 [doi]
AB  - OBJECTIVE: To study the difference of amniotic fluid stem cell potential at 
      different gestational age. MATERIALS AND METHODS: Second trimester amniocentesis 
      was performed during 15 to 22nd week of gestational age in a single medical 
      center from 2015 to 2016. Early second trimester amniotic fluid stem cells 
      (E-AFS) and later one (L-AFS) were defined 15-18th week, and 19-22nd week, 
      respectively. Cell characteristics, surface markers and ability to form induced 
      pluripotent stem cells (iPS) were studied. RESULTS: All the amniotic fluid stem 
      cells samples could be isolated and cultured from second trimester amniocentesis. 
      E-AFS showed more Ckit + cell, shorted doubling time, smaller cell size and 
      higher cell density compared to L-AFS. Both groups had the same stem cell surface 
      markers with highly expression of CD44, CD73, CD90, and CD105, negative for CD45. 
      They can easily be reprogramed into amniotic fluid stem cell derived iPS via 
      standard induction. CONCLUSION: Human amniotic fluid stem cells could be isolated 
      from early or late second trimester amniocentesis with the similar stem cell 
      surface markers presentation, especially in mesenchymal stem cells markers. 
      However, the cells from early second trimester amniocentesis have more 
      Ckit + number and more potential characteristics compared to late second 
      trimester amniocentesis. Both E-AFS and L-AFS could form the iPS easily which 
      lead to the future disease modeling study.
CI  - Copyright (c) 2017. Published by Elsevier B.V.
FAU - Shaw, S W Steven
AU  - Shaw SWS
AD  - Division of Obstetrics, Department of Obstetrics and Gynecology, Chang Gung 
      Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan; Division of Obstetrics, Department of 
      Obstetrics and Gynecology, Taipei Chang Gung Memorial Hospital, Taipei, Taiwan. 
      Electronic address: dr.shaw@me.com.
FAU - Cheng, Po-Jen
AU  - Cheng PJ
AD  - Division of Obstetrics, Department of Obstetrics and Gynecology, Chang Gung 
      Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
FAU - Chang, Yao-Lung
AU  - Chang YL
AD  - Division of Obstetrics, Department of Obstetrics and Gynecology, Chang Gung 
      Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
FAU - Chao, An-Shine
AU  - Chao AS
AD  - Division of Obstetrics, Department of Obstetrics and Gynecology, Chang Gung 
      Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
FAU - Wang, Tzu-Hao
AU  - Wang TH
AD  - Division of Obstetrics, Department of Obstetrics and Gynecology, Chang Gung 
      Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
FAU - Chang, Shuenn-Dyh
AU  - Chang SD
AD  - Division of Obstetrics, Department of Obstetrics and Gynecology, Chang Gung 
      Memorial Hospital Linkou Medical Center, Taoyuan, Taiwan; College of Medicine, 
      Chang Gung University, Taoyuan, Taiwan.
FAU - Hsieh, T'sang-T'ang
AU  - Hsieh TT
AD  - College of Medicine, Chang Gung University, Taoyuan, Taiwan; Division of 
      Obstetrics, Department of Obstetrics and Gynecology, Taipei Chang Gung Memorial 
      Hospital, Taipei, Taiwan.
FAU - Chang, Kuo-Hsuan
AU  - Chang KH
AD  - Department of Neurology, Chang Gung Memorial Hospital Linkou Medical Center, 
      Taoyuan, Taiwan.
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Taiwan J Obstet Gynecol
JT  - Taiwanese journal of obstetrics & gynecology
JID - 101213819
RN  - 0 (Membrane Proteins)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Amniocentesis
MH  - Amniotic Fluid/*cytology
MH  - Cellular Reprogramming/*physiology
MH  - Cellular Reprogramming Techniques/methods
MH  - Female
MH  - Gestational Age
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*physiology
MH  - Membrane Proteins/metabolism
MH  - Mesenchymal Stem Cells/*physiology
MH  - Pregnancy
MH  - Pregnancy Trimester, Second/*physiology
MH  - Proto-Oncogene Proteins c-kit/metabolism
MH  - Time Factors
OTO - NOTNLM
OT  - Amniotic fluid stem cells
OT  - Second trimester amniocentesis
OT  - iPS
EDAT- 2017/12/16 06:00
MHDA- 2018/08/02 06:00
CRDT- 2017/12/16 06:00
PHST- 2017/10/12 00:00 [accepted]
PHST- 2017/12/16 06:00 [entrez]
PHST- 2017/12/16 06:00 [pubmed]
PHST- 2018/08/02 06:00 [medline]
AID - S1028-4559(17)30251-6 [pii]
AID - 10.1016/j.tjog.2017.10.012 [doi]
PST - ppublish
SO  - Taiwan J Obstet Gynecol. 2017 Dec;56(6):770-774. doi: 10.1016/j.tjog.2017.10.012.