PMID- 29243125
OWN - NLM
STAT- MEDLINE
DCOM- 20180508
LR  - 20220321
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 472
IP  - 4
DP  - 2018 Apr
TI  - Minichromosome maintenance complex component 6 (MCM6) expression correlates with 
      histological grade and survival in endometrioid endometrial adenocarcinoma.
PG  - 623-633
LID - 10.1007/s00428-017-2278-9 [doi]
AB  - Minichromosome maintenance complex component 6 (MCM6) is involved in initiating 
      DNA replication and is upregulated during licensed G0 phase of the cell cycle. 
      This early expression permits its labeling of more proliferating cells than those 
      by Ki-67. Here using a cohort of 89 endometrioid adenocarcinoma, we report 
      findings made on the prognostic value of MCM6 based on immunohistochemical 
      labeling index (LI) of the protein in comparison with that of Ki67 as no such 
      information is currently available. Additionally, we examined the prognostic 
      values of these markers based on their mRNA expression using a cohort of uterine 
      corpus endometrial carcinoma (UCEC, n = 307) taken from The Cancer Genome Atlas 
      (TCGA) database. Our evidence indicated the presence of a positive correlation 
      between the LI of MCM6 and the histological grade of endometrioid endometrial 
      adenocarcinoma (grade I, 66.7%; grade II, 75.3%; grade III, 81.4%; p < 0.001) and 
      an inverse correlation between the LI of MCM6 and the overall and 
      progression-free survival (p = 0.02 for both). The LI of Ki-67 correlated with 
      grade (p < 0.001), but not survival. The MCM6 and Ki-67 inter-observer 
      intra-class correlation coefficients were excellent: 0.84 (95% confidence 
      interval, 0.83-0.91) and 0.84 (0.77-0.90), respectively. For in silico analyses 
      of the TCGA cohort, both univariate and multivariate Cox analyses (p = 0.003 and 
      p = 0.03, respectively) revealed high MCM6 mRNA Z-scores associated with reduced 
      overall survival. This association was absent for Ki-67. MCM6 is thus a highly 
      reproducible marker of poor prognosis in endometrial cancer. Evaluation of MCM6 
      should thus be considered in daily practice for risk stratification.
FAU - Hotton, Judicael
AU  - Hotton J
AD  - Department of Gynecology and Obstetrics, CHRU de Nancy, Nancy, France.
AD  - INSERM U954, Universite de Lorraine, Vandoeuvre-les-Nancy, France.
FAU - Agopiantz, Mikael
AU  - Agopiantz M
AD  - INSERM U954, Universite de Lorraine, Vandoeuvre-les-Nancy, France.
AD  - Department of Medical Gynecology, CHRU de Nancy, Nancy, France.
FAU - Leroux, Agnes
AU  - Leroux A
AD  - Department of Pathology, Institut de Cancerologie de Lorraine, 
      Vandoeuvre-les-Nancy, France.
FAU - Charra-Brunaud, Claire
AU  - Charra-Brunaud C
AD  - Department of Radiotherapy, Institut de Cancerologie de Lorraine, 
      Vandoeuvre-les-Nancy, France.
FAU - Marie, Beatrice
AU  - Marie B
AD  - Department of Pathology, CHRU de Nancy, Nancy, France.
FAU - Busby-Venner, Helene
AU  - Busby-Venner H
AD  - INSERM U954, Universite de Lorraine, Vandoeuvre-les-Nancy, France.
AD  - Department of Pathology, CHRU de Nancy, Nancy, France.
FAU - Morel, Olivier
AU  - Morel O
AD  - Department of Gynecology and Obstetrics, CHRU de Nancy, Nancy, France.
FAU - Gueant, Jean-Louis
AU  - Gueant JL
AD  - INSERM U954, Universite de Lorraine, Vandoeuvre-les-Nancy, France.
AD  - Department of Molecular Medicine and Personalized Therapeutics, Department of 
      Biochemistry, Molecular Biology, Nutrition, and Metabolism, CHRU de Nancy, 
      Vandoeuvre-les-Nancy, France.
FAU - Vignaud, Jean-Michel
AU  - Vignaud JM
AD  - INSERM U954, Universite de Lorraine, Vandoeuvre-les-Nancy, France.
AD  - Department of Pathology, CHRU de Nancy, Nancy, France.
FAU - Battaglia-Hsu, Shyue-Fang
AU  - Battaglia-Hsu SF
AD  - INSERM U954, Universite de Lorraine, Vandoeuvre-les-Nancy, France.
FAU - Gauchotte, Guillaume
AU  - Gauchotte G
AUID- ORCID: 0000-0001-5585-9195
AD  - INSERM U954, Universite de Lorraine, Vandoeuvre-les-Nancy, France. 
      g.gauchotte@chru-nancy.fr.
AD  - Department of Pathology, CHRU de Nancy, Nancy, France. g.gauchotte@chru-nancy.fr.
AD  - Service d'Anatomie et Cytologie Pathologiques, Hopital Central, CHRU de Nancy, 
      29, avenue du Marechal De Lattre de Tassigny, 54000, Nancy, France. 
      g.gauchotte@chru-nancy.fr.
LA  - eng
PT  - Journal Article
DEP - 20171214
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (Biomarkers, Tumor)
RN  - EC 3.6.4.12 (MCM6 protein, human)
RN  - EC 3.6.4.12 (Minichromosome Maintenance Complex Component 6)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/*analysis
MH  - Carcinoma, Endometrioid/mortality/*pathology
MH  - Cohort Studies
MH  - Disease-Free Survival
MH  - Endometrial Neoplasms/mortality/pathology
MH  - Female
MH  - Humans
MH  - Middle Aged
MH  - Minichromosome Maintenance Complex Component 6/analysis/*biosynthesis
MH  - Prognosis
MH  - Retrospective Studies
OTO - NOTNLM
OT  - Endometrial carcinoma
OT  - Endometrioid adenocarcinoma
OT  - Immunohistochemistry
OT  - Ki-67
OT  - MCM6
OT  - Proliferation
EDAT- 2017/12/16 06:00
MHDA- 2018/05/09 06:00
CRDT- 2017/12/16 06:00
PHST- 2017/09/05 00:00 [received]
PHST- 2017/12/04 00:00 [accepted]
PHST- 2017/11/30 00:00 [revised]
PHST- 2017/12/16 06:00 [pubmed]
PHST- 2018/05/09 06:00 [medline]
PHST- 2017/12/16 06:00 [entrez]
AID - 10.1007/s00428-017-2278-9 [pii]
AID - 10.1007/s00428-017-2278-9 [doi]
PST - ppublish
SO  - Virchows Arch. 2018 Apr;472(4):623-633. doi: 10.1007/s00428-017-2278-9. Epub 2017 
      Dec 14.