PMID- 29246132
OWN - NLM
STAT- MEDLINE
DCOM- 20180611
LR  - 20181202
IS  - 1471-2377 (Electronic)
IS  - 1471-2377 (Linking)
VI  - 17
IP  - 1
DP  - 2017 Dec 16
TI  - Neuroprotective effects of hyperbaric oxygen (HBO) therapy on neuronal death 
      induced by sciatic nerve transection in rat.
PG  - 220
LID - 10.1186/s12883-017-1004-1 [doi]
LID - 220
AB  - BACKGROUND: Recent studies shows that hyperbaric oxygen (HBO) therapy exerts some 
      protective effects against neural injuries. The purpose of this study was to 
      determine the neuroprotective effects of HBO following sciatic nerve transection 
      (SNT). METHODS: Rats were randomly divided into five groups (n = 14 per group): 
      Sham-operated (SH) group, SH + HBO group, SNT group, and SNT + pre- and 
      SNT + post-HBO groups (100% oxygen at 2.0 atm absolute, 60 min/day for five 
      consecutive days beginning on 1 day before and immediately after nerve 
      transaction, respectively). Spinal cord segments of the sciatic nerve and related 
      dorsal root ganglions (DRGs) were removed 4 weeks after nerve transection for 
      biochemical assessment of malodialdehyde (MDA) levels in spinal cord, biochemical 
      assessment of superoxide dismutase (SOD) and catalse (CAT) activities in spinal 
      cord, immunohistochemistry of caspase-3, cyclooxigenase-2 (COX-2), S100beta 
      (S100ss), and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) 
      in spinal cord and DRG. RESULTS: The results revealed that MDA levels were 
      significantly decreased in the SNT + pre-HBO group, while SOD and CAT activities 
      were significantly increased in SNT + pre- and SNT + post-HBO treated rats. 
      Attenuated caspase-3 and COX-2 expression, and TUNEL reaction could be 
      significantly detected in the HBO-treated rats after nerve transection. Also, HBO 
      significantly increased S100ss expression. CONCLUSIONS: Based on these results, we 
      can conclude that pre- and post-HBO therapy had neuroprotective effects against 
      sciatic nerve transection-induced degeneration.
FAU - Shams, Zahra
AU  - Shams Z
AD  - Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
FAU - Khalatbary, Ali Reza
AU  - Khalatbary AR
AD  - Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran. 
      khalat90@yahoo.com.
FAU - Ahmadvand, Hassan
AU  - Ahmadvand H
AD  - Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical 
      Sciences, Khorramabad, Iran.
AD  - Razi Herbal Researches Center, Lorestan University of Medical Sciences, 
      Khorramabad, Iran.
FAU - Zare, Zohreh
AU  - Zare Z
AD  - Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
FAU - Kian, Kosar
AU  - Kian K
AD  - Molecular and Cell Biology Research Center, Department of Anatomy, Faculty of 
      Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
LA  - eng
GR  - 2978/Mazandaran University of Medical Sciences/
PT  - Journal Article
DEP - 20171216
PL  - England
TA  - BMC Neurol
JT  - BMC neurology
JID - 100968555
RN  - 0 (Neuroprotective Agents)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Caspase 3/metabolism
MH  - Hyperbaric Oxygenation/*methods
MH  - Male
MH  - Neurons/*pathology
MH  - Neuroprotective Agents/*administration & dosage
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/*pathology
MH  - Spinal Cord/metabolism
PMC - PMC5732534
OTO - NOTNLM
OT  - Apoptosis
OT  - Hyperbaric oxygen
OT  - Inflammation
OT  - Nerve transaction
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Experimental procedures and protocols 
      used in this study were approved by ethical committee of Health Sciences, 
      Mazandaran University of Medical Sciences (IR.MAZUMS.REC.1396.2978). CONSENT FOR 
      PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they 
      have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2017/12/17 06:00
MHDA- 2018/06/12 06:00
PMCR- 2017/12/16
CRDT- 2017/12/17 06:00
PHST- 2017/10/30 00:00 [received]
PHST- 2017/12/06 00:00 [accepted]
PHST- 2017/12/17 06:00 [entrez]
PHST- 2017/12/17 06:00 [pubmed]
PHST- 2018/06/12 06:00 [medline]
PHST- 2017/12/16 00:00 [pmc-release]
AID - 10.1186/s12883-017-1004-1 [pii]
AID - 1004 [pii]
AID - 10.1186/s12883-017-1004-1 [doi]
PST - epublish
SO  - BMC Neurol. 2017 Dec 16;17(1):220. doi: 10.1186/s12883-017-1004-1.