PMID- 29246806
OWN - NLM
STAT- MEDLINE
DCOM- 20181212
LR  - 20181212
IS  - 1872-9428 (Electronic)
IS  - 0166-6851 (Linking)
VI  - 219
DP  - 2018 Jan
TI  - Characterization of three salmon louse (Lepeophtheirus salmonis) genes with 
      fibronectin II domains expressed by tegumental type 1 glands.
PG  - 1-9
LID - S0166-6851(17)30153-6 [pii]
LID - 10.1016/j.molbiopara.2017.12.002 [doi]
AB  - The salmon louse, Lepeophtheirus salmonis (Copepoda: Caligidae), is currently the 
      most significant pathogen affecting the salmon farming industry in the Northern 
      Hemisphere. Exocrine glands of blood-feeding parasites are believed to be 
      important for the host-parasite interaction, but also in the production of 
      substances for integument lubrication and antifouling. In L. salmonis; however, 
      we have limited knowledge about the exocrine glands. The aim of this study was 
      therefore to examine three genes containing fibronectin type II (FNII) domains 
      expressed in L. salmonis tegumental type 1 (teg 1) glands, namely LsFNII1, 2 and 
      3. LsFNII1, 2 and 3 contains four, three, and two FNII domains respectively. 
      Sequence alignment of LsFNII domains showed conservation of amino acids that may 
      indicate a possible involvement of LsFNII domains in collagen binding. 
      Ontogenetic analysis of LsFNII1, 2 and 3 revealed highest expression in pre-adult 
      and adult lice. Localization of LsFNII1, 2 and 3 transcripts showed expression in 
      teg 1 glands only, which are the most abundant exocrine gland type in L. 
      salmonis. LsFNII1, 2 and 3 were successfully knocked-down by RNAi, however, 
      alteration in gland morphology was not detected between the knock-down and 
      control groups. Overall, this study gives first insight into FNII domain 
      containing proteins in L. salmonis.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Harasimczuk, Ewa
AU  - Harasimczuk E
AD  - SLCR Sea Lice Research Center, Institute of Marine Research, 5817 Bergen, Norway. 
      Electronic address: Ewa.Harasimczuk@hi.no.
FAU - Overgard, Aina-Cathrine
AU  - Overgard AC
AD  - SLRC Sea Lice Research Center, Department of Biology, University of Bergen, 
      Thormohlensgt. 55, 5008 Bergen, Norway. Electronic address: 
      Cathrine.Overgard@uib.no.
FAU - Grotmol, Sindre
AU  - Grotmol S
AD  - SLRC Sea Lice Research Center, Department of Biology, University of Bergen, 
      Thormohlensgt. 55, 5008 Bergen, Norway. Electronic address: 
      Sindre.Grotmol@uib.no.
FAU - Nilsen, Frank
AU  - Nilsen F
AD  - SLRC Sea Lice Research Center, Department of Biology, University of Bergen, 
      Thormohlensgt. 55, 5008 Bergen, Norway. Electronic address: Frank.Nilsen@uib.no.
FAU - Dalvin, Sussie
AU  - Dalvin S
AD  - SLCR Sea Lice Research Center, Institute of Marine Research, 5817 Bergen, Norway. 
      Electronic address: Sussie.dalvin@hi.no.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171212
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Fibronectins)
SB  - IM
MH  - Animals
MH  - Conserved Sequence
MH  - Copepoda/*genetics/metabolism
MH  - Exocrine Glands/*metabolism
MH  - Fibronectins/*genetics
MH  - Gene Expression Profiling
MH  - Gene Knockdown Techniques
MH  - Sequence Homology, Amino Acid
OTO - NOTNLM
OT  - Crustacea
OT  - Exocrine glands
OT  - In situ hybridization
OT  - Integument
OT  - RNA interference
OT  - Sea lice
EDAT- 2017/12/17 06:00
MHDA- 2018/12/13 06:00
CRDT- 2017/12/17 06:00
PHST- 2017/04/05 00:00 [received]
PHST- 2017/10/30 00:00 [revised]
PHST- 2017/12/11 00:00 [accepted]
PHST- 2017/12/17 06:00 [pubmed]
PHST- 2018/12/13 06:00 [medline]
PHST- 2017/12/17 06:00 [entrez]
AID - S0166-6851(17)30153-6 [pii]
AID - 10.1016/j.molbiopara.2017.12.002 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 2018 Jan;219:1-9. doi: 10.1016/j.molbiopara.2017.12.002. 
      Epub 2017 Dec 12.