PMID- 2924724
OWN - NLM
STAT- MEDLINE
DCOM- 19890503
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 124
IP  - 4
DP  - 1989 Apr
TI  - Dilute estradiol implants and progestin receptor induction in the ventromedial 
      nucleus of the hypothalamus: correlation with receptive behavior in female rats.
PG  - 1807-12
AB  - The ventromedial nucleus of the hypothalamus (VMN) is thought to constitute an 
      essential neural substrate for hormonal induction of female sexual behavior. In 
      previous work, implants of dilute (0.4%) estradiol (E2) have been found to prime 
      progesterone-facilitated female sexual behavior in rats only when they are within 
      or very close to the VMN. In other studies, induction of cytosolic progestin 
      receptors (PRc) in the hypothalamus has been correlated with systemic E2 priming 
      of receptive behavior. We combined dilute E2 implants with a Palkovits punch 
      microassay for PRc in the an effort to examine the relationship between estrous 
      behavior and estrogen-induced PRc in the VMN of individual female rats. 
      Ovariectomized rats were given bilateral guide cannulae aimed at the VMN and were 
      primed for 3 days with 1) blank implants plus oil sc, 2) blank implants plus 0.5 
      micrograms E2 benzoate sc, 3) implants of 0.4% E2 in cholesterol, or 4) implants 
      of 2.0% E2. On day 4 all rats received progesterone (P) sc and were tested for 
      receptive behavior. The following week all subjects received the same 
      estrogen-priming treatment but were killed on day 4 without P treatment. The VMN 
      was punch dissected and assayed for PRc. High levels of lordosis were seen in the 
      E2 benzoate-treated and 2.0% E2-implanted animals, while little behavior was seen 
      in the oil-treated animals. Among 0.4% E2-implanted animals, higher levels of 
      lordosis were observed in subjects with implant placements in the rostral than in 
      the caudal VMN; however, lordosis quotients were not correlated with PRc 
      induction measured in the entire VMN of individual animals. In general, our 
      findings suggest that local estrogenic stimulation of target cells in the VMN is 
      capable of both priming the estrous response and induction of P receptors. 
      Whether these two effects are causally related awaits future study.
FAU - Pleim, E T
AU  - Pleim ET
AD  - Department of Biological Sciences, Rutgers University, New Brunswick, New Jersey 
      08903.
FAU - Brown, T J
AU  - Brown TJ
FAU - MacLusky, N J
AU  - MacLusky NJ
FAU - Etgen, A M
AU  - Etgen AM
FAU - Barfield, R J
AU  - Barfield RJ
LA  - eng
GR  - HD-4-04484/HD/NICHD NIH HHS/United States
GR  - NS-07807/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Drug Implants)
RN  - 0 (Receptors, Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Drug Implants
MH  - Estradiol/*administration & dosage/pharmacology
MH  - Female
MH  - Gene Expression Regulation
MH  - Ovariectomy
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Receptors, Progesterone/analysis/genetics/*metabolism
MH  - Sexual Behavior, Animal/*drug effects
MH  - Ventromedial Hypothalamic Nucleus/analysis/*drug effects/ultrastructure
EDAT- 1989/04/01 00:00
MHDA- 1989/04/01 00:01
CRDT- 1989/04/01 00:00
PHST- 1989/04/01 00:00 [pubmed]
PHST- 1989/04/01 00:01 [medline]
PHST- 1989/04/01 00:00 [entrez]
AID - 10.1210/endo-124-4-1807 [doi]
PST - ppublish
SO  - Endocrinology. 1989 Apr;124(4):1807-12. doi: 10.1210/endo-124-4-1807.