PMID- 29247241
OWN - NLM
STAT- MEDLINE
DCOM- 20190709
LR  - 20211204
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Dec 15
TI  - Beneficial effects of melittin on ovalbumin-induced atopic dermatitis in mouse.
PG  - 17679
LID - 10.1038/s41598-017-17873-2 [doi]
LID - 17679
AB  - Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense 
      pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in 
      the epidermal barrier and immunoglobulin E (IgE)-mediated sensitization to 
      several environmental allergens, as well as an immune disorder mediated by an 
      imbalance toward T-helper-2 response. Melittin, a major component of bee venom, 
      has been studied in various inflammatory diseases. However, the beneficial 
      effects of melittin on mouse with AD-like symptoms have not been explored. 
      Therefore, we investigated the anti-allergic effects of melittin. AD was induced 
      by ovalbumin (OVA) patch. After agent treatment, skin tissues and sera were 
      extracted from the sacrificed mice were used to demonstrate the effects of 
      melittin through various molecular biological methods. The results showed that 
      OVA-induced skin thickening and inflammatory infiltration were decreased in the 
      melittin-treated group. Melittin prevented OVA-induced filaggrin deficiency and 
      imbalanced inflammatory mediators. Furthermore, melittin inhibited 
      IL-4/IL-13-induced filaggrin downregulation through the blockade of STAT3 
      activation in human keratinocytes. In summary, this study has shown that melittin 
      ameliorated OVA-induced AD-like symptoms from various perspectives. The findings 
      of this study may be the first evidence of the anti-inflammatory effects of 
      melittin on OVA-induced AD.
FAU - Kim, Woon-Hae
AU  - Kim WH
AUID- ORCID: 0000-0002-1536-5374
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea.
FAU - An, Hyun-Jin
AU  - An HJ
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea.
FAU - Kim, Jung-Yeon
AU  - Kim JY
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea.
FAU - Gwon, Mi-Gyeong
AU  - Gwon MG
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea.
FAU - Gu, Hyemin
AU  - Gu H
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea.
FAU - Jeon, Minji
AU  - Jeon M
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea.
FAU - Sung, Woo Jung
AU  - Sung WJ
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea.
FAU - Han, Sang Mi
AU  - Han SM
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Jeonju-si, Korea.
FAU - Pak, Sok Cheon
AU  - Pak SC
AD  - School of Biomedical Sciences, Charles Sturt University, Bathurst, Australia.
FAU - Kim, Min-Kyung
AU  - Kim MK
AD  - Department of Pathology, College of Medicine, Dongguk University, Gyeongju-si, 
      Korea.
FAU - Park, Kwan-Kyu
AU  - Park KK
AUID- ORCID: 0000-0002-5317-750X
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, 
      Daegu, Korea. kkpark@cu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171215
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Allergens)
RN  - 0 (FLG protein, human)
RN  - 0 (Filaggrin Proteins)
RN  - 0 (Interleukin-13)
RN  - 0 (STAT3 Transcription Factor)
RN  - 20449-79-0 (Melitten)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens/pharmacology
MH  - Animals
MH  - Cells, Cultured
MH  - Dermatitis, Atopic/*chemically induced/*drug therapy/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Filaggrin Proteins
MH  - Immunoglobulin E/metabolism
MH  - Interleukin-13/metabolism
MH  - Keratinocytes/drug effects/metabolism
MH  - Melitten/*pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/*pharmacology
MH  - STAT3 Transcription Factor/metabolism
MH  - Skin/drug effects/metabolism
PMC - PMC5732199
COIS- The authors declare that they have no competing interests.
EDAT- 2017/12/17 06:00
MHDA- 2019/07/10 06:00
PMCR- 2017/12/15
CRDT- 2017/12/17 06:00
PHST- 2017/07/31 00:00 [received]
PHST- 2017/12/01 00:00 [accepted]
PHST- 2017/12/17 06:00 [entrez]
PHST- 2017/12/17 06:00 [pubmed]
PHST- 2019/07/10 06:00 [medline]
PHST- 2017/12/15 00:00 [pmc-release]
AID - 10.1038/s41598-017-17873-2 [pii]
AID - 17873 [pii]
AID - 10.1038/s41598-017-17873-2 [doi]
PST - epublish
SO  - Sci Rep. 2017 Dec 15;7(1):17679. doi: 10.1038/s41598-017-17873-2.