PMID- 29247548
OWN - NLM
STAT- MEDLINE
DCOM- 20190122
LR  - 20190122
IS  - 1098-2744 (Electronic)
IS  - 0899-1987 (Linking)
VI  - 57
IP  - 4
DP  - 2018 Apr
TI  - Extracellular vesicles as drivers of epithelial-mesenchymal transition and 
      carcinogenic characteristics in normal prostate cells.
PG  - 503-511
LID - 10.1002/mc.22775 [doi]
AB  - There is increasing evidence that cancer dissemination and metastasis 
      establishment may not only be due to the movement of tumor cells. Content of 
      extracellular vesicles (EVs) secreted by tumor cells may also reflect the origin 
      of these cells. Some molecules that constitute these EVs have already been used 
      as targets for detection of specific tumors. However, to the best of our 
      knowledge, EVs from biopsies and plasma have not yet been compared nor thoroughly 
      investigated as triggers of malignant transformation and metastatic niche 
      formation. To evaluate the role of EVs in the cellular microenvironment, we have 
      treated the normal epithelial prostate cell lines, RWPE-1 and PNT-2, with a pool 
      of EVs from biopsies of prostate primary tumors (bEVs), biopsies of benign 
      prostate hyperplasia (hEVs), plasma of prostate cancer (PCa) patients (pEVs) or 
      plasma of healthy individuals (pnEVs). Each of the four pools consisted of 
      isolated EVs from several subjects, of which PCa patients were in different 
      stages of cancer. Migration and proliferation profiles, cytokine release, and a 
      panel of PCa-associated genes' expression of epithelial-mesenchymal transition in 
      the cell lines were evaluated after 24 h incubation with EVs. When compared to 
      the control groups, cells treated with the pool of EVs isolated from tumor 
      biopsies and plasma of PCa patients showed greater migration and proliferation, 
      significant alterations in gene expression, and high levels of IL-8, factors that 
      are associated with cancer development. Specifically, isolated bEVs and pEVs may 
      induce malignant features in non-tumor cells by activating several cellular 
      events associated with cancer progression, suggesting that future PCa therapy may 
      target multiple elements found in tumor-derived EVs.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Souza, Aline G
AU  - Souza AG
AUID- ORCID: 0000-0001-7251-7285
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
FAU - B Silva, Isaura Beatriz
AU  - B Silva IB
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
FAU - Campos-Fernandez, Esther
AU  - Campos-Fernandez E
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
FAU - Marangoni, Karina
AU  - Marangoni K
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
FAU - F Bastos, Victor A
AU  - F Bastos VA
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
FAU - Alves, Patricia T
AU  - Alves PT
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
FAU - Goulart, Luiz R
AU  - Goulart LR
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
AD  - Department of Medical Microbiology and Immunology, University of 
      California-Davis, Davis, California.
FAU - Alonso-Goulart, Vivian
AU  - Alonso-Goulart V
AD  - Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University 
      of Uberlandia, MG, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180105
PL  - United States
TA  - Mol Carcinog
JT  - Molecular carcinogenesis
JID - 8811105
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Line
MH  - Cell Movement/genetics
MH  - Cell Transformation, Neoplastic/*genetics
MH  - Epithelial-Mesenchymal Transition/*genetics
MH  - Extracellular Vesicles/*genetics/ultrastructure
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Microscopy, Electron, Scanning
MH  - Middle Aged
MH  - Prostate/cytology/*metabolism
MH  - Prostatic Neoplasms/genetics/pathology
MH  - Tumor Cells, Cultured
MH  - Young Adult
OTO - NOTNLM
OT  - carcinogenesis
OT  - epithelial-mesenchymal transition
OT  - malignant transformation
OT  - metastasis prostate cancer
EDAT- 2017/12/17 06:00
MHDA- 2019/01/23 06:00
CRDT- 2017/12/17 06:00
PHST- 2017/08/11 00:00 [received]
PHST- 2017/12/08 00:00 [revised]
PHST- 2017/12/11 00:00 [accepted]
PHST- 2017/12/17 06:00 [pubmed]
PHST- 2019/01/23 06:00 [medline]
PHST- 2017/12/17 06:00 [entrez]
AID - 10.1002/mc.22775 [doi]
PST - ppublish
SO  - Mol Carcinog. 2018 Apr;57(4):503-511. doi: 10.1002/mc.22775. Epub 2018 Jan 5.