PMID- 29247691
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20180831
IS  - 1873-3441 (Electronic)
IS  - 0939-6411 (Linking)
VI  - 124
DP  - 2018 Mar
TI  - Single dose HBsAg CS-gamma-PGA nanogels induce potent protective immune responses 
      against HBV infection.
PG  - 82-88
LID - S0939-6411(17)30812-3 [pii]
LID - 10.1016/j.ejpb.2017.12.003 [doi]
AB  - Hepatitis B virus (HBV) infection is a severe threat to public health, which can 
      be prevented by prophylactic vaccination. Here, we tested nanogels carriers in 
      the prophylactic effect of hepatitis B surface antigen (HBsAg) vaccine. HBsAg 
      nanogels (Ng) were prepared using chitosan (CS) and poly-gamma-glutamic acid (gamma-PGA). 
      Positively charged Ng (+) and negatively charged Ng (-) were prepared by 
      adjusting the CS and gamma-PGA proportion. Dendritic cells (DCs) maturation in mice 
      immunized with HBsAg Ng (+) and HBsAg Ng (-) could be augmented in response to 
      pAAV/HBV1.2 plasmid challenge. Single-dose immunization with HBsAg Ng (+) induced 
      HBsAg specific-antibodies. HBsAg Ng (+) immunized mice cleared HBsAg and restored 
      anti-HBs production after pAAV/HBV1.2 plasmid challenge. Single-dose HBsAg Ng (+) 
      induced humoral and cellular immunity, and could induce effector memory T cells. 
      Single-dose HBsAg Ng (-) favored the induction of cellular immunity, and induced 
      central memory T cells and effector memory T cells. However, HBsAg elimination 
      was similar between HBsAg Ng (+)- and HBsAg Ng (+) plus HBsAg Ng (-)-immunized 
      mice. Zeta potential measurements showed that HBsAg Ng (+) were more stable than 
      HBsAg Ng (-). Therefore, Ng (+) are desirable HBsAg prophylactic vaccine 
      carriers, providing long-term protection against HBV, and are a good choice to 
      study and apply weakly immunostimulatory antigens.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Wang, Haigang
AU  - Wang H
AD  - Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, 
      Shandong University, China.
FAU - Han, Qiuju
AU  - Han Q
AD  - Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, 
      Shandong University, China.
FAU - Zhao, Huajun
AU  - Zhao H
AD  - Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, 
      Shandong University, China.
FAU - Xu, Dongqing
AU  - Xu D
AD  - Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, 
      Shandong University, China.
FAU - Zhang, Jian
AU  - Zhang J
AD  - Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, 
      Shandong University, China. Electronic address: zhangj65@sdu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20171213
PL  - Netherlands
TA  - Eur J Pharm Biopharm
JT  - European journal of pharmaceutics and biopharmaceutics : official journal of 
      Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
JID - 9109778
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Drug Carriers)
RN  - 0 (Gels)
RN  - 0 (Hepatitis B Antibodies)
RN  - 0 (Hepatitis B Surface Antigens)
RN  - 0 (Hepatitis B Vaccines)
RN  - 25513-46-6 (Polyglutamic Acid)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage/chemistry
MH  - Animals
MH  - Chitosan/*administration & dosage/chemistry/immunology
MH  - Dendritic Cells/drug effects/immunology/metabolism
MH  - Disease Models, Animal
MH  - *Drug Carriers
MH  - Drug Compounding
MH  - Gels
MH  - Hepatitis B/blood/immunology/*prevention & control/virology
MH  - Hepatitis B Antibodies/blood
MH  - Hepatitis B Surface Antigens/*administration & dosage/chemistry/immunology
MH  - Hepatitis B Vaccines/*administration & dosage/chemistry/immunology
MH  - Immunity, Cellular/drug effects
MH  - Immunity, Humoral/drug effects
MH  - Immunization
MH  - Immunogenicity, Vaccine
MH  - Immunologic Memory/drug effects
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Nanomedicine/methods
MH  - *Nanoparticles
MH  - Polyglutamic Acid/*administration & dosage/chemistry/immunology
MH  - T-Lymphocyte Subsets/drug effects/immunology/metabolism
MH  - Technology, Pharmaceutical/methods
MH  - Time Factors
OTO - NOTNLM
OT  - Chitosan
OT  - HBsAg
OT  - Nanogels
OT  - Poly-gamma-glutamic acid
OT  - Vaccine
EDAT- 2017/12/17 06:00
MHDA- 2018/09/01 06:00
CRDT- 2017/12/17 06:00
PHST- 2017/07/24 00:00 [received]
PHST- 2017/10/28 00:00 [revised]
PHST- 2017/12/17 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
PHST- 2017/12/17 06:00 [entrez]
AID - S0939-6411(17)30812-3 [pii]
AID - 10.1016/j.ejpb.2017.12.003 [doi]
PST - ppublish
SO  - Eur J Pharm Biopharm. 2018 Mar;124:82-88. doi: 10.1016/j.ejpb.2017.12.003. Epub 
      2017 Dec 13.