PMID- 29248374
OWN - NLM
STAT- MEDLINE
DCOM- 20190923
LR  - 20200306
IS  - 2405-4712 (Print)
IS  - 2405-4720 (Electronic)
IS  - 2405-4712 (Linking)
VI  - 6
IP  - 1
DP  - 2018 Jan 24
TI  - Multi-omics Reveal Specific Targets of the RNA-Binding Protein Puf3p and Its 
      Orchestration of Mitochondrial Biogenesis.
PG  - 125-135.e6
LID - S2405-4712(17)30540-9 [pii]
LID - 10.1016/j.cels.2017.11.012 [doi]
AB  - Coenzyme Q (CoQ) is a redox-active lipid required for mitochondrial oxidative 
      phosphorylation (OxPhos). How CoQ biosynthesis is coordinated with the biogenesis 
      of OxPhos protein complexes is unclear. Here, we show that the Saccharomyces 
      cerevisiae RNA-binding protein (RBP) Puf3p regulates CoQ biosynthesis. To 
      establish the mechanism for this regulation, we employed a multi-omic strategy to 
      identify mRNAs that not only bind Puf3p but also are regulated by Puf3p in vivo. 
      The CoQ biosynthesis enzyme Coq5p is a critical Puf3p target: Puf3p regulates the 
      abundance of Coq5p and prevents its detrimental hyperaccumulation, thereby 
      enabling efficient CoQ production. More broadly, Puf3p represses a specific set 
      of proteins involved in mitochondrial protein import, translation, and OxPhos 
      complex assembly (pathways essential to prime mitochondrial biogenesis). Our data 
      reveal a mechanism for post-transcriptionally coordinating CoQ production with 
      OxPhos biogenesis, and they demonstrate the power of multi-omics for defining 
      genuine targets of RBPs.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Lapointe, Christopher P
AU  - Lapointe CP
AD  - Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, 
      USA.
FAU - Stefely, Jonathan A
AU  - Stefely JA
AD  - Morgridge Institute for Research, Madison, WI 53715, USA.
FAU - Jochem, Adam
AU  - Jochem A
AD  - Morgridge Institute for Research, Madison, WI 53715, USA.
FAU - Hutchins, Paul D
AU  - Hutchins PD
AD  - Genome Center of Wisconsin, Madison, WI 53706, USA; Department of Chemistry, 
      University of Wisconsin-Madison, Madison, WI 53706, USA.
FAU - Wilson, Gary M
AU  - Wilson GM
AD  - Genome Center of Wisconsin, Madison, WI 53706, USA; Department of Chemistry, 
      University of Wisconsin-Madison, Madison, WI 53706, USA.
FAU - Kwiecien, Nicholas W
AU  - Kwiecien NW
AD  - Genome Center of Wisconsin, Madison, WI 53706, USA; Department of Chemistry, 
      University of Wisconsin-Madison, Madison, WI 53706, USA.
FAU - Coon, Joshua J
AU  - Coon JJ
AD  - Genome Center of Wisconsin, Madison, WI 53706, USA; Department of Chemistry, 
      University of Wisconsin-Madison, Madison, WI 53706, USA; Department of 
      Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
FAU - Wickens, Marvin
AU  - Wickens M
AD  - Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, 
      USA. Electronic address: wickens@biochem.wisc.edu.
FAU - Pagliarini, David J
AU  - Pagliarini DJ
AD  - Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, 
      USA; Morgridge Institute for Research, Madison, WI 53715, USA. Electronic 
      address: dpagliarini@morgridge.org.
LA  - eng
GR  - F30 AG043282/AG/NIA NIH HHS/United States
GR  - R01 GM112057/GM/NIGMS NIH HHS/United States
GR  - T32 GM008692/GM/NIGMS NIH HHS/United States
GR  - R35 GM118110/GM/NIGMS NIH HHS/United States
GR  - R01 GM050942/GM/NIGMS NIH HHS/United States
GR  - T32 GM008349/GM/NIGMS NIH HHS/United States
GR  - R01 GM115591/GM/NIGMS NIH HHS/United States
GR  - P41 GM108538/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20171213
PL  - United States
TA  - Cell Syst
JT  - Cell systems
JID - 101656080
RN  - 0 (PUF3 protein, S cerevisiae)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 1339-63-5 (Ubiquinone)
SB  - IM
MH  - Mitochondria/enzymology
MH  - Organelle Biogenesis
MH  - Oxidative Phosphorylation
MH  - RNA-Binding Proteins/*genetics/*metabolism
MH  - Saccharomyces cerevisiae/enzymology/genetics
MH  - Saccharomyces cerevisiae Proteins/*genetics/*metabolism
MH  - Ubiquinone/biosynthesis
PMC - PMC5799006
MID - NIHMS922863
OTO - NOTNLM
OT  - *COQ5
OT  - *PUF3
OT  - *RNA binding protein
OT  - *biogenesis
OT  - *coenzyme Q
OT  - *complex Q
OT  - *mitochondria
OT  - *multi-omic
OT  - *transomic
OT  - *ubiquinone
EDAT- 2017/12/19 06:00
MHDA- 2019/09/24 06:00
PMCR- 2019/01/24
CRDT- 2017/12/18 06:00
PHST- 2017/05/31 00:00 [received]
PHST- 2017/09/14 00:00 [revised]
PHST- 2017/11/19 00:00 [accepted]
PHST- 2017/12/19 06:00 [pubmed]
PHST- 2019/09/24 06:00 [medline]
PHST- 2017/12/18 06:00 [entrez]
PHST- 2019/01/24 00:00 [pmc-release]
AID - S2405-4712(17)30540-9 [pii]
AID - 10.1016/j.cels.2017.11.012 [doi]
PST - ppublish
SO  - Cell Syst. 2018 Jan 24;6(1):125-135.e6. doi: 10.1016/j.cels.2017.11.012. Epub 
      2017 Dec 13.