PMID- 29248592
OWN - NLM
STAT- MEDLINE
DCOM- 20180918
LR  - 20180918
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 47
DP  - 2018 Mar
TI  - Fluorochloridone induces primary cultured Sertoli cells apoptosis: Involvement of 
      ROS and intracellular calcium ions-mediated ERK1/2 activation.
PG  - 228-237
LID - S0887-2333(17)30372-7 [pii]
LID - 10.1016/j.tiv.2017.12.006 [doi]
AB  - Fluorochloridone (FLC) is a widely used pyrrolidone selective herbicide and 
      reported to induce testis injuries in male rats, but the underlying mechanism is 
      largely unknown. In the present study, primary-cultured Sertoli cells were 
      exposed to FLC at the concentration of 0-10.00muM to study the mechanism of 
      FLC-induced apoptosis. The roles of ROS, intracellular calcium, endoplasmic 
      reticulum (ER), and ERK1/2 were looked at with ROS scavenger N-acetyl-cysteine 
      (NAC), intracellular calcium chelator BAPTA-AM, ER calcium depleting agent 
      thapsigargin (TG), and ERK1/2 inhibitor U0126, respectively. FLC induced 
      dose-dependent apoptosis increase as well as the elevation in levels of ROS, 
      intracellular calcium, and ERK1/2 activation. FLC treatment led to constantly 
      increasing apoptotic rates and ERK1/2 activation over time, while inversed-V 
      shaped change tendencies of ROS and intracellular calcium levels were observed. 
      FLC-induced ROS generation disrupted the intracellular calcium homeostasis by 
      attacking the ER, and the elevated intracellular calcium levels resulted in 
      ERK1/2 over-phosphorylation and consequently promoted Sertoli cell apoptosis. 
      Taken together, ROS and intracellular calcium-mediated ERK1/2 activation led to 
      FLC-induced Sertoli cell apoptosis.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Liu, Luqing
AU  - Liu L
AD  - School of Public Health/MOE Key Laboratory for Public Health Safety/Collaborative 
      Innovation Center of Social Risks Governance in Health, Fudan University, 
      Shanghai 200032, China.
FAU - Chang, Xiuli
AU  - Chang X
AD  - School of Public Health/MOE Key Laboratory for Public Health Safety/Collaborative 
      Innovation Center of Social Risks Governance in Health, Fudan University, 
      Shanghai 200032, China.
FAU - Zhang, Yubin
AU  - Zhang Y
AD  - School of Public Health/MOE Key Laboratory for Public Health Safety/Collaborative 
      Innovation Center of Social Risks Governance in Health, Fudan University, 
      Shanghai 200032, China.
FAU - Wu, Chunhua
AU  - Wu C
AD  - School of Public Health/MOE Key Laboratory for Public Health Safety/Collaborative 
      Innovation Center of Social Risks Governance in Health, Fudan University, 
      Shanghai 200032, China.
FAU - Li, Rui
AU  - Li R
AD  - School of Public Health/MOE Key Laboratory for Public Health Safety/Collaborative 
      Innovation Center of Social Risks Governance in Health, Fudan University, 
      Shanghai 200032, China; Pharmacology and Toxicology Department, Shanghai 
      Institute for Food and Drug Control, Shanghai 201203, China.
FAU - Tang, Liming
AU  - Tang L
AD  - Pharmacology and Toxicology Department, Shanghai Institute for Food and Drug 
      Control, Shanghai 201203, China.
FAU - Zhou, Zhijun
AU  - Zhou Z
AD  - School of Public Health/MOE Key Laboratory for Public Health Safety/Collaborative 
      Innovation Center of Social Risks Governance in Health, Fudan University, 
      Shanghai 200032, China. Electronic address: zjzhou@fudan.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20171214
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Antioxidants)
RN  - 0 (Calcium Chelating Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Herbicides)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Pyrrolidinones)
RN  - 0 (Reactive Oxygen Species)
RN  - AO023ZB1BP (raiser)
RN  - EC 3.6.3.8 (Sarcoplasmic Reticulum Calcium-Transporting ATPases)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Apoptosis/*drug effects
MH  - Calcium Chelating Agents/pharmacology
MH  - Calcium Signaling/*drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Endoplasmic Reticulum/drug effects/enzymology/metabolism
MH  - Enzyme Activation/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Herbicides/*toxicity
MH  - Hormesis
MH  - MAP Kinase Signaling System/*drug effects
MH  - Male
MH  - Phosphorylation/drug effects
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Protein Processing, Post-Translational/drug effects
MH  - Pyrrolidinones/*toxicity
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/*metabolism
MH  - Sarcoplasmic Reticulum Calcium-Transporting ATPases/antagonists & 
      inhibitors/metabolism
MH  - Sertoli Cells/cytology/*drug effects
OTO - NOTNLM
OT  - Apoptosis
OT  - ERK1/2
OT  - Fluorochloridone
OT  - Intracellular calcium
OT  - ROS
EDAT- 2017/12/19 06:00
MHDA- 2018/09/19 06:00
CRDT- 2017/12/18 06:00
PHST- 2017/08/03 00:00 [received]
PHST- 2017/11/18 00:00 [revised]
PHST- 2017/12/12 00:00 [accepted]
PHST- 2017/12/19 06:00 [pubmed]
PHST- 2018/09/19 06:00 [medline]
PHST- 2017/12/18 06:00 [entrez]
AID - S0887-2333(17)30372-7 [pii]
AID - 10.1016/j.tiv.2017.12.006 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2018 Mar;47:228-237. doi: 10.1016/j.tiv.2017.12.006. Epub 2017 
      Dec 14.