PMID- 29248768
OWN - NLM
STAT- MEDLINE
DCOM- 20180302
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1073
DP  - 2018 Jan 15
TI  - Measurement of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 
      8-oxo-7,8-dihydro-guanosine in cerebrospinal fluid by ultra performance liquid 
      chromatography-tandem mass spectrometry.
PG  - 110-117
LID - S1570-0232(17)31027-9 [pii]
LID - 10.1016/j.jchromb.2017.12.011 [doi]
AB  - Increased levels of nucleosides modified by oxidation in human cerebrospinal 
      fluid (CSF) have several times been reported in Alzheimer patients and patients 
      suffering from Parkinson's disease. The focus has especially been on nucleosides 
      containing the 8-hydroxylation of guanine. Only few reports on quantification of 
      the ribonucleoside 8-oxo-7,8-dihydro-guanosine (8oxoGuo) in CSF have been 
      published, whereas more have been published on the quantification of the 
      deoxy-ribonucleoside 8-oxo-7,8-dihydro-2'-deoxyguanosine (8oxodGuo). The reports 
      on the quantification of 8oxodGuo concentrations in CSF report absolute 
      concentrations varying by a factor >10(5) in healthy humans. This could indicate 
      that there is a serious specificity problem in some of the methods. In this paper 
      an isotope-dilution UPLC-MS/MS method with high specificity and sensitivity for 
      the quantification of 8oxoGuo and 8oxodGuo in CSF is presented. LLOQ for the two 
      analytes is determined to 4pM and 2pM, respectively. The calibration curves has 
      been tested to be linear in the range from 4 to 3,000pM for 8oxoGuo and between 2 
      and 3,000pM for 8oxodGuo. Using a weighting factor of 1/x the correlation 
      coefficient "r" for both analytes is >0.999.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Weimann, Allan
AU  - Weimann A
AD  - Laboratory of Clinical Pharmacology Q7642, Rigshospitalet, Blegdamsvej 9, DK-2100 
      Copenhagen O, Denmark; Department of Clinical Pharmacology, Bispebjerg and 
      Frederiksberg Hospital, DK-2400 Copenhagen NV, Denmark. Electronic address: 
      aweimann@rh.dk.
FAU - Simonsen, Anja Hviid
AU  - Simonsen AH
AD  - Danish Dementia Research Centre, Rigshospitalet, University of Copenhagen, 
      Section 6991, Blegdamsvej 9, DK-2100 Copenhagen O, Denmark.
FAU - Poulsen, Henrik E
AU  - Poulsen HE
AD  - Laboratory of Clinical Pharmacology Q7642, Rigshospitalet, Blegdamsvej 9, DK-2100 
      Copenhagen O, Denmark; Department of Clinical Pharmacology, Bispebjerg and 
      Frederiksberg Hospital, DK-2400 Copenhagen NV, Denmark; Faculty of Medicine, 
      University of Copenhagen, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20171209
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 12133JR80S (Guanosine)
RN  - 3868-31-3 (8-hydroxyguanosine)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Aged
MH  - Child
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Deoxyguanosine/*analogs & derivatives/cerebrospinal fluid
MH  - Guanosine/*analogs & derivatives/cerebrospinal fluid
MH  - Humans
MH  - Linear Models
MH  - Middle Aged
MH  - Oxidative Stress
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - 8-Hydroxy-2'-deoxyguanosine
OT  - 8-Hydroxyguanosine
OT  - Cerebrospinal fluid
OT  - Liquid chromatography
OT  - Mass spectrometry
OT  - Oxidative stress
EDAT- 2017/12/19 06:00
MHDA- 2018/03/03 06:00
CRDT- 2017/12/18 06:00
PHST- 2017/06/07 00:00 [received]
PHST- 2017/12/04 00:00 [revised]
PHST- 2017/12/07 00:00 [accepted]
PHST- 2017/12/19 06:00 [pubmed]
PHST- 2018/03/03 06:00 [medline]
PHST- 2017/12/18 06:00 [entrez]
AID - S1570-0232(17)31027-9 [pii]
AID - 10.1016/j.jchromb.2017.12.011 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 15;1073:110-117. doi: 
      10.1016/j.jchromb.2017.12.011. Epub 2017 Dec 9.