PMID- 29248928
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20210109
IS  - 1662-8128 (Electronic)
IS  - 1662-811X (Print)
IS  - 1662-811X (Linking)
VI  - 10
IP  - 2
DP  - 2018
TI  - Differential Kinetics of Aspergillus nidulans and Aspergillus fumigatus 
      Phagocytosis.
PG  - 145-160
LID - 10.1159/000484562 [doi]
AB  - Invasive aspergillosis mainly occurs in immunocompromised patients and is 
      commonly caused by Aspergillus fumigatus, while A.nidulans is rarely the 
      causative agent. However, in chronic granulomatous disease (CGD) patients, A. 
      nidulans is a frequent cause of invasive aspergillosis and is associated with 
      higher mortality. Immune recognition of A. nidulans was compared to A. fumigatus 
      to offer an insight into why A. nidulans infections are prevalent in CGD. Live 
      cell imaging with J774A.1 macrophage-like cells and LC3-GFP-mCherry bone 
      marrow-derived macrophages (BMDMs) revealed that phagocytosis of A. nidulans was 
      slower compared to A. fumigatus. This difference could be attributed to slower 
      migration of J774A.1 cells and a lower percentage of migrating BMDMs. In 
      addition, delayed phagosome acidification and LC3-associated phagocytosis was 
      observed with A. nidulans. Cytokine and oxidative burst measurements in human 
      peripheral blood mononuclear cells revealed a lower oxidative burst upon 
      challenge with A. nidulans. In contrast, A. nidulans induced significantly higher 
      concentrations of cytokines. Collectively, our data demonstrate that A. nidulans 
      is phagocytosed and processed at a slower rate compared to A. fumigatus, 
      resulting in reduced fungal killing and increased germination of conidia. This 
      slower rate of A. nidulans clearance may be permissive for overgrowth within 
      certain immune settings.
CI  - The Author(s). Published by S. Karger AG, Basel.
FAU - Gresnigt, Mark S
AU  - Gresnigt MS
AD  - Medical Research Council Centre for Medical Mycology at the University of 
      Aberdeen, Aberdeen Fungal Group, Institute of Medical Sciences, University of 
      Aberdeen, Aberdeen, UK.
FAU - Becker, Katharina L
AU  - Becker KL
FAU - Leenders, Floris
AU  - Leenders F
FAU - Alonso, M Fernanda
AU  - Alonso MF
FAU - Wang, Xiaowen
AU  - Wang X
FAU - Meis, Jacques F
AU  - Meis JF
FAU - Bain, Judith M
AU  - Bain JM
FAU - Erwig, Lars P
AU  - Erwig LP
FAU - van de Veerdonk, Frank L
AU  - van de Veerdonk FL
LA  - eng
GR  - MR/N006364/1/MRC_/Medical Research Council/United Kingdom
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171216
PL  - Switzerland
TA  - J Innate Immun
JT  - Journal of innate immunity
JID - 101469471
RN  - 0 (Cytokines)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - Aspergillosis/immunology/microbiology
MH  - Aspergillus fumigatus/*immunology
MH  - Aspergillus nidulans/*immunology
MH  - Cell Line
MH  - Cell Movement
MH  - Cytokines/metabolism
MH  - Granulomatous Disease, Chronic/immunology/microbiology
MH  - Humans
MH  - Kinetics
MH  - Leukocytes, Mononuclear/metabolism/microbiology
MH  - Macrophages/metabolism/microbiology
MH  - Mice
MH  - *Phagocytosis
MH  - Phagosomes/metabolism/microbiology
MH  - Reactive Oxygen Species/metabolism
MH  - Species Specificity
PMC - PMC6757152
OTO - NOTNLM
OT  - <italic>Aspergillus fumigatus</italic>
OT  - <italic>Aspergillus nidulans</italic>
OT  - Chronic granulomatous disease
OT  - LC3-associated phagocytosis
OT  - Phagocytosis
OT  - Phagosome acidification
EDAT- 2017/12/19 06:00
MHDA- 2019/09/10 06:00
PMCR- 2017/12/16
CRDT- 2017/12/18 06:00
PHST- 2017/04/07 00:00 [received]
PHST- 2017/10/25 00:00 [accepted]
PHST- 2017/12/19 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2017/12/18 06:00 [entrez]
PHST- 2017/12/16 00:00 [pmc-release]
AID - 000484562 [pii]
AID - jin-0010-0145 [pii]
AID - 10.1159/000484562 [doi]
PST - ppublish
SO  - J Innate Immun. 2018;10(2):145-160. doi: 10.1159/000484562. Epub 2017 Dec 16.