PMID- 29250120
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2017
DP  - 2017
TI  - Human Chorionic Plate-Derived Mesenchymal Stem Cells Restore Hepatic Lipid 
      Metabolism in a Rat Model of Bile Duct Ligation.
PG  - 5180579
LID - 10.1155/2017/5180579 [doi]
LID - 5180579
AB  - In cholestatic liver diseases, impaired bile excretion disrupts lipid 
      homeostasis. We investigated changes of lipid metabolism, including mitochondrial 
      beta-oxidation, in a rat model of bile duct ligation (BDL) in which chorionic 
      plate-derived mesenchymal stem cells (CP-MSCs) were transplanted. Serum 
      cholesterol level, which was elevated after BDL, was significantly decreased 
      following CP-MSC transplantation. The expression levels of genes involved in 
      intracellular lipid uptake, including long-chain fatty acyl-CoA synthetases and 
      fatty acid transport proteins, were decreased in rats after BDL; however, they 
      were not significantly changed by subsequent CP-MSC transplantation. Carnitine 
      palmitoyltransferase 1A (CPT1A), a rate-limiting enzyme in mitochondrial 
      beta-oxidation, was upregulated after BDL and then was downregulated after CP-MSC 
      transplantation. CPT1A expression was changed via microRNA-33-a 
      posttranscriptional regulator of CPT1A-in a peroxisome proliferator-activated 
      receptor alpha-independent manner. Cellular adenosine triphosphate production-an 
      indicator of mitochondrial function-was reduced after BDL and was restored by 
      CP-MSC transplantation. Expression levels of heme oxygenases also were 
      significantly affected following BDL and CP-MSC transplantation. Lipid metabolism 
      is altered in response to chronic cholestatic liver injury and can be restored by 
      CP-MSC transplantation. Our study findings support the therapeutic potential of 
      CP-MSCs in cholestatic liver diseases and help in understanding the fundamental 
      mechanisms by which CP-MSCs affect energy metabolism.
FAU - Lee, Yun Bin
AU  - Lee YB
AUID- ORCID: 0000-0002-3193-9745
AD  - Department of Internal Medicine, CHA Bundang Medical Center, CHA University, 
      Seongnam, Republic of Korea.
FAU - Choi, Jong Ho
AU  - Choi JH
AD  - Department of Biomedical Science, CHA University, Seongnam, Republic of Korea.
AD  - Department of Dermatology, The Feinberg School of Medicine, Northwestern 
      University, Chicago, IL, USA.
FAU - Kim, Eun Nam
AU  - Kim EN
AD  - Department of Internal Medicine, CHA Bundang Medical Center, CHA University, 
      Seongnam, Republic of Korea.
AD  - Clinical Research Center, CHA Bundang Medical Center, CHA University, Seongnam, 
      Republic of Korea.
FAU - Seok, Jin
AU  - Seok J
AD  - Department of Biomedical Science, CHA University, Seongnam, Republic of Korea.
FAU - Lee, Hyun-Jung
AU  - Lee HJ
AD  - Department of Biomedical Science, CHA University, Seongnam, Republic of Korea.
FAU - Yoon, Jung-Hwan
AU  - Yoon JH
AUID- ORCID: 0000-0002-9128-3610
AD  - Department of Internal Medicine and Liver Research Institute, Seoul National 
      University College of Medicine, Seoul, Republic of Korea.
FAU - Kim, Gi Jin
AU  - Kim GJ
AUID- ORCID: 0000-0002-2320-7157
AD  - Department of Biomedical Science, CHA University, Seongnam, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20171109
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC5700509
EDAT- 2017/12/19 06:00
MHDA- 2017/12/19 06:01
PMCR- 2017/11/09
CRDT- 2017/12/19 06:00
PHST- 2017/07/28 00:00 [received]
PHST- 2017/10/02 00:00 [accepted]
PHST- 2017/12/19 06:00 [entrez]
PHST- 2017/12/19 06:00 [pubmed]
PHST- 2017/12/19 06:01 [medline]
PHST- 2017/11/09 00:00 [pmc-release]
AID - 10.1155/2017/5180579 [doi]
PST - ppublish
SO  - Stem Cells Int. 2017;2017:5180579. doi: 10.1155/2017/5180579. Epub 2017 Nov 9.