PMID- 29251705
OWN - NLM
STAT- MEDLINE
DCOM- 20190304
LR  - 20190304
IS  - 2163-0763 (Electronic)
IS  - 2163-0755 (Linking)
VI  - 84
IP  - 5
DP  - 2018 May
TI  - Human adipose-derived stem cell treatment modulates cellular protection in both 
      in vitro and in vivo traumatic brain injury models.
PG  - 745-751
LID - 10.1097/TA.0000000000001770 [doi]
AB  - BACKGROUND: Traumatic brain injury (TBI) is a common cause of morbidity and 
      mortality in the civilian population. The purpose of this study was to examine 
      the effect(s) of adipose-derived stem cell (ASC) treatment on cellular and 
      functional recovery in TBI via both in vitro and in vivo methods. METHODS: 
      Cultured neuroblastoma cells, SH-SY5Y, were scratched to mimic TBI in an in vitro 
      model. The effect of ASC-conditioned medium (CM) on cell death, mitochondrial 
      function, and expression of inflammatory cytokines (tumor necrosis factor alpha 
      [TNF-alpha], interleukin 1beta [IL-1beta], and IL-6), as well as apoptosis marker FAS, was 
      measured. In our in vivo model, Sprague-Dawley rats underwent TBI via a frontal, 
      closed-head injury model. Animals randomly received either intravenous 
      human-derived ASCs or intravenous saline within 3 hours of injury and were 
      compared with a sham group. Functional recovery was evaluated via accelerating 
      Rotarod method. On post-TBI Day 3, brain tissue was harvested and assessed for 
      cellular damage via enzyme-linked immunosorbent assay for TNF-alpha, as well as 
      immunohistochemical staining for beta-amyloid precursor protein (beta-APP). RESULTS: 
      Our in vitro data show that ASC treatment imparted reduced cell death (ratio to 
      control: 1.21 +/- 0.066 vs. 1.01 +/- 0.056, p = 0.017), increased cell viability 
      (ratio to control: 0.86 +/- 0.009 vs. 1.09 +/- 0.01, p = 0.0001), increased 
      mitochondrial function (percentage of control: 78 +/- 6% vs. 68 +/- 3%), and 
      significantly decreased levels of inflammatory cytokine IL-1beta. In our in vivo 
      study, compared with TBI alone, ASC-treated animals showed no difference in 
      functional recovery, lower levels of expressed TNF-alpha (ratio to total protein, 
      0.47 +/- 0.01 vs. 0.67 +/- 0.04; p < 0.01), and lower levels of beta-amyloid precursor 
      protein (fluorescence ratio, 0.43 +/- 0.05 vs. 0.69 +/- 0.03; p < 0.01). CONCLUSIONS: 
      Adipose-derived stem cell treatment results in improved cell survival, decreased 
      inflammatory marker release, and decreased evidence of neural injury. No 
      difference in functional recovery was seen. These data suggest the potential for 
      ASC treatment to aid in cellular protection and recovery in neural cells 
      following TBI.
FAU - Kappy, Nikolas S
AU  - Kappy NS
AD  - From the Department of Surgery (N.S.K., S.C., W.M.H., M.P., T.O., P.Z., J.P.C., 
      S.A.B.), Cooper University Hospital, Camden, NJ; and Division of Trauma (J.P.H.), 
      Cooper University Hospital, Camden, NJ.
FAU - Chang, Shaohua
AU  - Chang S
FAU - Harris, William M
AU  - Harris WM
FAU - Plastini, Michael
AU  - Plastini M
FAU - Ortiz, Telisha
AU  - Ortiz T
FAU - Zhang, Ping
AU  - Zhang P
FAU - Hazelton, Joshua P
AU  - Hazelton JP
FAU - Carpenter, Jeffrey P
AU  - Carpenter JP
FAU - Brown, Spencer A
AU  - Brown SA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Trauma Acute Care Surg
JT  - The journal of trauma and acute care surgery
JID - 101570622
RN  - 0 (Cytokines)
SB  - IM
MH  - Adipocytes/*cytology
MH  - Animals
MH  - Brain Injuries, Traumatic/metabolism/pathology/*therapy
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Humans
MH  - Male
MH  - Nerve Regeneration/*physiology
MH  - Neurons/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cell Transplantation/*methods
MH  - Stem Cells/*cytology
MH  - Treatment Outcome
EDAT- 2017/12/19 06:00
MHDA- 2019/03/05 06:00
CRDT- 2017/12/19 06:00
PHST- 2017/12/19 06:00 [pubmed]
PHST- 2019/03/05 06:00 [medline]
PHST- 2017/12/19 06:00 [entrez]
AID - 10.1097/TA.0000000000001770 [doi]
PST - ppublish
SO  - J Trauma Acute Care Surg. 2018 May;84(5):745-751. doi: 
      10.1097/TA.0000000000001770.