PMID- 29253819
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20180820
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 55
DP  - 2018 Feb
TI  - Mouse beta-defensin-14 for inducing the maturation of dendritic cells.
PG  - 133-141
LID - S1567-5769(17)30490-3 [pii]
LID - 10.1016/j.intimp.2017.12.017 [doi]
AB  - BACKGROUND: beta-defensins are an excellent antimicrobial peptide against microbial 
      infection in which dendritic cells (DCs) play a crucial role by improving the 
      innate and adaptive immune defense. However, it is unclear whether BDs affect DC 
      maturation. This work aimed to study the effects of mouse beta-defensin-14 (MBD-14) 
      on DC maturation. METHODS: Via in vitro using mouse bone marrow DCs, the 
      maturation of DCs was evaluated by cell morphological staining, flow cytometry, 
      endocytosis assay, and allogeneic mixed lymphocyte reaction, respectively. And it 
      was also assessed by in vivo establishing a mouse air-pouch model for flow 
      cytometric determination, cytokine analysis, and histological staining. 
      Additionally, CLI-095, an inhibitor of Toll-like receptor-4 (TLR-4), was used to 
      determine whether TLR-4 is possibly involved in DC maturation. RESULTS: It was 
      found MBD-14 promoted DCs to form more filopodia and lamellipodia, increased the 
      expression of DC maturation markers (CD40 and MHC-II), decreased their endocytic 
      capacity, and enhanced T-cell proliferation. The analyses of the air-pouch 
      exudates were consistent with the in vitro results of MBD-14 activating DCs. And 
      when CLI-095 was applied, DC maturation was inhibited partly. CONCLUSIONS: This 
      work demonstrates that MBD-14 can promote the maturation of DCs in which TLR-4 is 
      possibly involved.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Yuan, Xiangwei
AU  - Yuan X
AD  - Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong 
      University, Shanghai 200233, China.
FAU - Wang, Jiaxing
AU  - Wang J
AD  - Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong 
      University, Shanghai 200233, China.
FAU - Cheng, Mengqi
AU  - Cheng M
AD  - Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong 
      University, Shanghai 200233, China.
FAU - Zhang, Xianlong
AU  - Zhang X
AD  - Department of Orthopedics, Shanghai Sixth People's Hospital, Shanghai Jiao Tong 
      University, Shanghai 200233, China. Electronic address: 
      dr_zhangxianlong@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20171222
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (CD40 Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Sulfonamides)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (beta-Defensins)
RN  - 0 (beta-defensin-14, mouse)
RN  - 0 (ethyl 6-(N-(2-chloro-4-fluorophenyl)sulfamoyl)cyclohex-1-ene-1-carboxylate)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - CD40 Antigens/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dendritic Cells/*physiology
MH  - Endocytosis
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Sulfonamides/pharmacology
MH  - T-Lymphocytes/*immunology
MH  - Toll-Like Receptor 4/antagonists & inhibitors/metabolism
MH  - beta-Defensins/*metabolism
OTO - NOTNLM
OT  - Antimicrobial peptide
OT  - Dendritic cell
OT  - Immune regulation
OT  - Mouse beta-defensin-14
EDAT- 2017/12/19 06:00
MHDA- 2018/08/21 06:00
CRDT- 2017/12/19 06:00
PHST- 2017/10/24 00:00 [received]
PHST- 2017/12/12 00:00 [revised]
PHST- 2017/12/12 00:00 [accepted]
PHST- 2017/12/19 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PHST- 2017/12/19 06:00 [entrez]
AID - S1567-5769(17)30490-3 [pii]
AID - 10.1016/j.intimp.2017.12.017 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2018 Feb;55:133-141. doi: 10.1016/j.intimp.2017.12.017. Epub 
      2017 Dec 22.