PMID- 29254562 OWN - NLM STAT- MEDLINE DCOM- 20180814 LR - 20191210 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 999 DP - 2018 Jan 25 TI - A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification. PG - 123-131 LID - S0003-2670(17)31209-6 [pii] LID - 10.1016/j.aca.2017.10.032 [doi] AB - A simple and highly sensitive immunoassay based on a competitive binding and bio-barcode amplification was designed for detection of small molecules, triazophos. The gold nanoparticles (AuNPs) were modified with monoclonal antibodies and 6-carboxyfluorescein labeled single-stranded thiol-oligonucleotides (6-FAM-SH-ssDNAs); the fluorescence of 6-FAM was quenched by AuNPs. Ovalbumin-linked haptens were coated on the bottom of microplate to compete with the triazophos in the sample for binding to the antibodies on the AuNP probes. The fluorescence intensity was inversely proportional to analyte concentration. Parameters of AuNP probes preparation and immune reaction were optimized. At the optimal conditions, the salting process was shortened to 1 h and 166 +/- 9 ssDNAs were loaded onto a single AuNP. The competitive fluorescence bio-barcode immunoassay was performed on water, rice, cucumber, cabbage and apple samples. The linear range of the method was 0.01-20 mug L(-1)and the limit of detection (LOD) was 6 ng L(-1). The recovery and relative standard deviations (RSDs) ranged from 85.0 to 110.3% and 9.4-17.4%, respectively. Good correlations were obtained between the results of the developed method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In conclusion, it is suggested that the competitive fluorescent bio-barcode immunoassay had the potential to be used as a sensitive method for detection of a variety of small molecules in various samples. CI - Copyright (c) 2017 Elsevier B.V. All rights reserved. FAU - Zhang, Chan AU - Zhang C AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Du, Pengfei AU - Du P AD - Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences; Key Laboratory of Agro-Products Processing Technology of Shandong Province; Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, 202 Gongye North Road, Jinan 250100, PR China. FAU - Jiang, Zejun AU - Jiang Z AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Jin, Maojun AU - Jin M AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: katonking@163.com. FAU - Chen, Ge AU - Chen G AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Cao, Xiaolin AU - Cao X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Cui, Xueyan AU - Cui X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Zhang, Yudan AU - Zhang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Li, Ruixing AU - Li R AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, South Korea. FAU - Wang, Jing AU - Wang J AD - Institute of Quality Standard and Testing Technology for Agro-Products, Key Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural Sciences; Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing 100081, PR China. Electronic address: w_jing2001@126.com. LA - eng PT - Journal Article PT - Validation Study DEP - 20171030 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Antibodies, Immobilized) RN - 0 (DNA, Single-Stranded) RN - 0 (Insecticides) RN - 0 (Organothiophosphates) RN - 0 (Triazoles) RN - 0 (Water Pollutants, Chemical) RN - 059QF0KO0R (Water) RN - 6099J8L0EE (triazophos) RN - 7440-57-5 (Gold) SB - IM MH - Antibodies, Immobilized/*chemistry MH - DNA, Single-Stranded/chemistry MH - Environmental Monitoring/methods MH - Gold/*chemistry MH - Immunoassay/*methods MH - Insecticides/*analysis MH - Limit of Detection MH - Malus/chemistry MH - Metal Nanoparticles/*chemistry/ultrastructure MH - Organothiophosphates/*analysis MH - Oryza/chemistry MH - Triazoles/*analysis MH - Vegetables/chemistry MH - Water/analysis MH - Water Pollutants, Chemical/analysis OTO - NOTNLM OT - Bio-barcode OT - Fluorescence OT - Gold nanoparticles OT - Immunoassay OT - Triazophos EDAT- 2017/12/20 06:00 MHDA- 2018/08/15 06:00 CRDT- 2017/12/20 06:00 PHST- 2017/08/21 00:00 [received] PHST- 2017/10/20 00:00 [revised] PHST- 2017/10/26 00:00 [accepted] PHST- 2017/12/20 06:00 [entrez] PHST- 2017/12/20 06:00 [pubmed] PHST- 2018/08/15 06:00 [medline] AID - S0003-2670(17)31209-6 [pii] AID - 10.1016/j.aca.2017.10.032 [doi] PST - ppublish SO - Anal Chim Acta. 2018 Jan 25;999:123-131. doi: 10.1016/j.aca.2017.10.032. Epub 2017 Oct 30.