PMID- 29254577
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20191210
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 999
DP  - 2018 Jan 25
TI  - An integrated method for simultaneously determining 10 classes of per- and 
      polyfluoroalkyl substances in one drop of human serum.
PG  - 76-86
LID - S0003-2670(17)31248-5 [pii]
LID - 10.1016/j.aca.2017.10.038 [doi]
AB  - Per- and polyfluoroalkyl substances (PFASs) represent a group of synthetic 
      chemicals, and they have quite different physicochemical properties, which result 
      in difficulties of their simultaneous determination in a single injection. A 
      sensitive, reliable, and fully automated method was developed for simultaneously 
      detecting 10 classes of PFASs (total of 43) in human serum using online Turboflow 
      SPE-UHPLC-MS/MS. This method provided high linearity of matrix-matched 
      calibration standards (R > 0.99), excellent method limits of detection (MLODs) 
      (0.013-0.089 ng mL(-1)), satisfactory matrix spiked recoveries (84.3-109%) and 
      relative standard deviations (RSDs) (intra-day RSDs: 1.3-12.6%, inter-day RSDs: 
      1.7-13.8%, inter-week RSDs: 1.8-13.5%, inter-month RSDs: 3.1-12.4%), short 
      analysis time (19 min per sample) and small sample amount requirement (25 muL), 
      which were suitable for large-scale epidemiologic studies. Moreover, the method 
      provided the feasibility of real-time monitoring for the degradation kinetics of 
      PFASs precursors both in vitro and in vivo. The quality of the present method was 
      further verified by repetitive analysis of a standard reference material (SRM 
      1957), with the deviations of the targeted PFAS concentrations ranging from 1.9% 
      to 14.2% (n = 5) between the detected and reference values. The present study 
      also determined values for several PFASs in SRM 1957 other than those on the 
      certificate, for the first time, such as N-EtFOSA, 6:2 Cl-PFESA, and PFBA. 
      Finally, the established method was applied to detect PFASs in serum samples of 
      15 ordinary people and 15 occupational workers, and 6:2 FTSA was found as the 
      dominant precursor.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Gao, Ke
AU  - Gao K
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy of Sciences, Beijing 100049, China.
FAU - Fu, Jianjie
AU  - Fu J
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy of Sciences, Beijing 100049, China. Electronic address: jjfu@rcees.ac.cn.
FAU - Xue, Qiao
AU  - Xue Q
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Li, Yili
AU  - Li Y
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
FAU - Liang, Yong
AU  - Liang Y
AD  - Institute of Environment and Health, Jianghan University, Wuhan 430056, China.
FAU - Pan, Yuanyuan
AU  - Pan Y
AD  - Thermofisher Scientific Corporation, Beijing 100080, China.
FAU - Zhang, Aiqian
AU  - Zhang A
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China; College of Resources and Environment, University of Chinese 
      Academy of Sciences, Beijing 100049, China. Electronic address: 
      aqzhang@rcees.ac.cn.
FAU - Jiang, Guibin
AU  - Jiang G
AD  - State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research 
      Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 
      100085, China.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20171109
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Environmental Exposure/analysis
MH  - Environmental Pollutants/*blood
MH  - Fluorocarbons/*blood
MH  - Humans
MH  - Limit of Detection
MH  - Sample Size
MH  - Solid Phase Extraction/methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Fluorinated precursors
OT  - Online turboflow SPE
OT  - Per-and poly-fluoroalkyl substances
OT  - Perfluoroalkyl phosphonates
OT  - Polyfluoroalkyl phosphate esters
OT  - SRM 1957
EDAT- 2017/12/20 06:00
MHDA- 2018/08/15 06:00
CRDT- 2017/12/20 06:00
PHST- 2017/07/27 00:00 [received]
PHST- 2017/10/26 00:00 [revised]
PHST- 2017/10/29 00:00 [accepted]
PHST- 2017/12/20 06:00 [entrez]
PHST- 2017/12/20 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
AID - S0003-2670(17)31248-5 [pii]
AID - 10.1016/j.aca.2017.10.038 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2018 Jan 25;999:76-86. doi: 10.1016/j.aca.2017.10.038. Epub 2017 
      Nov 9.