PMID- 29255796
OWN - NLM
STAT- MEDLINE
DCOM- 20180807
LR  - 20220321
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 4
IP  - 6
DP  - 2017 Nov-Dec
TI  - The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA 
      Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons.
LID - 10.1523/ENEURO.0268-17.2017 [doi]
LID - ENEURO.0268-17.2017
AB  - Brain-derived neurotrophic factor (BDNF) is an important mediator of long-term 
      synaptic potentiation (LTP) in the hippocampus. The local effects of BDNF depend 
      on the activation of translation activity, which requires the delivery of 
      transcripts to the synapse. In this work, we found that neuronal activity 
      regulates the dendritic localization of the RNA-binding protein heterogeneous 
      nuclear ribonucleoprotein K (hnRNP K) in cultured rat hippocampal neurons by 
      stimulating BDNF-Trk signaling. Microarray experiments identified a large number 
      of transcripts that are coimmunoprecipitated with hnRNP K, and about 60% of these 
      transcripts are dissociated from the protein upon stimulation of rat hippocampal 
      neurons with BDNF. In vivo studies also showed a role for TrkB signaling in the 
      dissociation of transcripts from hnRNP K upon high-frequency stimulation (HFS) of 
      medial perforant path-granule cell synapses of male rat dentate gyrus (DG). 
      Furthermore, treatment of rat hippocampal synaptoneurosomes with BDNF decreased 
      the coimmunoprecipitation of hnRNP K with mRNAs coding for glutamate receptor 
      subunits, Ca(2+)- and calmodulin-dependent protein kinase IIbeta (CaMKIIbeta) and BDNF. 
      Downregulation of hnRNP K impaired the BDNF-induced enhancement of NMDA receptor 
      (NMDAR)-mediated mEPSC, and similar results were obtained upon inhibition of 
      protein synthesis with cycloheximide. The results demonstrate that BDNF regulates 
      specific populations of hnRNP-associated mRNAs in neuronal dendrites and suggests 
      an important role of hnRNP K in BDNF-dependent forms of synaptic plasticity.
FAU - Leal, Graciano
AU  - Leal G
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Comprido, Diogo
AU  - Comprido D
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - de Luca, Pasqualino
AU  - de Luca P
AUID- ORCID: 0000-0002-1113-2135
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Morais, Eduardo
AU  - Morais E
AUID- ORCID: 0000-0002-1103-2070
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Rodrigues, Luis
AU  - Rodrigues L
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Mele, Miranda
AU  - Mele M
AUID- ORCID: 0000-0002-8627-4883
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Santos, Ana R
AU  - Santos AR
AUID- ORCID: 0000-0002-0437-1146
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Costa, Rui O
AU  - Costa RO
AUID- ORCID: 0000-0002-2652-7500
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
AD  - Institute for Interdisciplinary Research, University of Coimbra, 3030-789 
      Coimbra, Portugal.
FAU - Pinto, Maria Joana
AU  - Pinto MJ
AUID- ORCID: 0000-0001-9730-5772
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Patil, Sudarshan
AU  - Patil S
AUID- ORCID: 0000-0002-6294-7959
AD  - Department of Biomedicine and KG Jebsen Centre for Neuropsychiatric Disorders, 
      University of Bergen, 5020 Bergen, Norway.
FAU - Berentsen, Birgitte
AU  - Berentsen B
AD  - Department of Biomedicine and KG Jebsen Centre for Neuropsychiatric Disorders, 
      University of Bergen, 5020 Bergen, Norway.
FAU - Afonso, Pedro
AU  - Afonso P
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
FAU - Carreto, Laura
AU  - Carreto L
AD  - Department of Medical Sciences and Institute of Biomedicine - iBiMED, University 
      of Aveiro, 3810-193 Aveiro, Portugal.
FAU - Li, Ka Wan
AU  - Li KW
AUID- ORCID: 0000-0001-6983-5055
AD  - Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije 
      Universiteit, 1081 Amsterdam, The Netherlands.
FAU - Pinheiro, Paulo
AU  - Pinheiro P
AUID- ORCID: 0000-0002-9024-1021
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
AD  - Institute for Interdisciplinary Research, University of Coimbra, 3030-789 
      Coimbra, Portugal.
FAU - Almeida, Ramiro D
AU  - Almeida RD
AUID- ORCID: 0000-0001-5830-2595
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
AD  - Institute for Interdisciplinary Research, University of Coimbra, 3030-789 
      Coimbra, Portugal.
AD  - Department of Medical Sciences and Institute of Biomedicine - iBiMED, University 
      of Aveiro, 3810-193 Aveiro, Portugal.
FAU - Santos, Manuel A S
AU  - Santos MAS
AUID- ORCID: 0000-0002-0437-1146
AD  - Department of Medical Sciences and Institute of Biomedicine - iBiMED, University 
      of Aveiro, 3810-193 Aveiro, Portugal.
FAU - Bramham, Clive R
AU  - Bramham CR
AUID- ORCID: 0000-0001-5958-7115
AD  - Department of Biomedicine and KG Jebsen Centre for Neuropsychiatric Disorders, 
      University of Bergen, 5020 Bergen, Norway.
FAU - Duarte, Carlos B
AU  - Duarte CB
AUID- ORCID: 0000-0003-1603-6721
AD  - CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 
      Coimbra, Portugal.
AD  - Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20171212
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Heterogeneous-Nuclear Ribonucleoprotein K)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Animals
MH  - Animals, Outbred Strains
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cells, Cultured
MH  - Dendrites/*metabolism
MH  - Excitatory Postsynaptic Potentials/physiology
MH  - Female
MH  - HEK293 Cells
MH  - Heterogeneous-Nuclear Ribonucleoprotein K/genetics/*metabolism
MH  - Hippocampus/cytology/*metabolism
MH  - Humans
MH  - Male
MH  - Microarray Analysis
MH  - Microelectrodes
MH  - RNA Transport/physiology
MH  - RNA, Messenger/metabolism
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Synapses/*metabolism
MH  - Synaptosomes/metabolism
PMC - PMC5732018
OTO - NOTNLM
OT  - BDNF
OT  - NMDA receptors
OT  - RNA transport
OT  - local translation
OT  - long-term synaptic potentiation
OT  - neurotrophins
EDAT- 2017/12/20 06:00
MHDA- 2018/08/08 06:00
PMCR- 2017/12/12
CRDT- 2017/12/20 06:00
PHST- 2017/08/01 00:00 [received]
PHST- 2017/10/25 00:00 [revised]
PHST- 2017/11/09 00:00 [accepted]
PHST- 2017/12/20 06:00 [entrez]
PHST- 2017/12/20 06:00 [pubmed]
PHST- 2018/08/08 06:00 [medline]
PHST- 2017/12/12 00:00 [pmc-release]
AID - eN-NWR-0268-17 [pii]
AID - 10.1523/ENEURO.0268-17.2017 [doi]
PST - epublish
SO  - eNeuro. 2017 Dec 12;4(6):ENEURO.0268-17.2017. doi: 10.1523/ENEURO.0268-17.2017. 
      eCollection 2017 Nov-Dec.