PMID- 29256235
OWN - NLM
STAT- MEDLINE
DCOM- 20190510
LR  - 20230805
IS  - 1008-9292 (Print)
IS  - 1008-9292 (Linking)
VI  - 46
IP  - 4
DP  - 2017 Jul 25
TI  - [Volume of 4% neutral buffered formaldehyde affects the results of HER2 gene 
      detected by fluorescence in situ hybridization in primary invasive breast 
      cancer].
PG  - 439-444
AB  - OBJECTIVE: To investigate the effects of the volume of 4% neutral phosphate 
      buffered formalin fixative solution on the detection of human epidermal growth 
      factor receptor 2 (HER2) gene by fluorescence in situ hybridization (FISH) in 
      primary invasive breast cancer. METHODS: Tissue samples were collected from 109 
      patients with primary invasive breast cancer admitted in Zhongshan Boai Hospital 
      from June 2014 to October 2016. The ratios of 4% phosphate buffered formalin 
      fixative solution to sample volume samples were 3:1, 6:1, 9:1, 10:1, 15:1, 20:1 
      or 25:1 (groups A, B, C, D, E, F and G), respectively. Paraffin sections were 
      made after 15 h of fixation. The amplification of HER2 gene was detected by FISH. 
      The gene amplification results of HER2 were observed and compared in different 
      groups. RESULTS: Fluorescence microscope showed that the tissue contour in groups 
      A, B and C was vague, cell debris appeared, and the probe was positioned poorly; 
      while the tissue contour was clear and complete in groups D, E, F and G and the 
      probe was positioned accurately. The positive rate of HER2 was gradually 
      increased from group A to D(chi(2)=8.601, P<0.01), and that remained stable at 
      24.77% in groups D to G. The positive rate of gene amplification in groups D, E, 
      F and G was significantly higher than that in groups A, B and C (all P<0.05). 
      CONCLUSIONS: When using FISH to detect HER2 gene in samples of primary breast 
      invasive carcinoma, the volume of fixative solution should be at least 10 times 
      of the sample volume to obtain accurate and stable results.
FAU - Chen, Zhiqiang
AU  - Chen Z
AD  - Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical 
      University, Zhongshan 528400, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical 
      University, Zhongshan 528400, China.
FAU - Mi, Xianjun
AU  - Mi X
AD  - Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical 
      University, Zhongshan 528400, China.
FAU - Duan, Lifeng
AU  - Duan L
AD  - Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical 
      University, Zhongshan 528400, China.
FAU - Chen, Ang
AU  - Chen A
AD  - Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical 
      University, Zhongshan 528400, China.
FAU - Huang, Huayong
AU  - Huang H
AD  - Department of Pathology, Zhongshan Boai Hospital Affiliated to Southern Medical 
      University, Zhongshan 528400, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhejiang Da Xue Xue Bao Yi Xue Ban
JT  - Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical 
      sciences
JID - 100927946
RN  - 0 (Buffers)
RN  - 1HG84L3525 (Formaldehyde)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/diagnosis
MH  - Buffers
MH  - *Formaldehyde/pharmacology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods/standards
MH  - *Receptor, ErbB-2/metabolism
MH  - Reproducibility of Results
PMC - PMC10396947
EDAT- 2017/12/20 06:00
MHDA- 2019/05/11 06:00
PMCR- 2017/08/25
CRDT- 2017/12/20 06:00
PHST- 2017/12/20 06:00 [entrez]
PHST- 2017/12/20 06:00 [pubmed]
PHST- 2019/05/11 06:00 [medline]
PHST- 2017/08/25 00:00 [pmc-release]
AID - zjdxxbyxb-46-4-439 [pii]
AID - 10.3785/j.issn.1008-9292.2017.08.15 [doi]
PST - ppublish
SO  - Zhejiang Da Xue Xue Bao Yi Xue Ban. 2017 Jul 25;46(4):439-444. doi: 
      10.3785/j.issn.1008-9292.2017.08.15.