PMID- 29256352
OWN - NLM
STAT- MEDLINE
DCOM- 20190611
LR  - 20190613
IS  - 1875-5666 (Electronic)
IS  - 1566-5240 (Linking)
VI  - 17
IP  - 7
DP  - 2018 Mar 9
TI  - Puerarin Stimulates Osteogenic Differentiation and Bone Formation Through the 
      ERK1/2 and p38-MAPK Signaling Pathways.
PG  - 488-496
LID - 10.2174/1566524018666171219101142 [doi]
AB  - BACKGROUND: Osteoporosis is a world-wide health problem, which leads to decreased 
      bone strength and increased susceptibility to fractures. Puerarin, a 
      phytoestrogen extracted from Pueraria lobata (Willd.) Ohwi, has been identified 
      as a promising intervention for preventing bone loss and promoting bone 
      regeneration. However, the underlying mechanisms for its anabolic action are 
      still not clear. In the present study, we aimed to investigate the effect of 
      puerarin on the osteogenic differentiation of bone marrow stromal cells (BMSCs) 
      and the possible molecular mechanism mediating its action. METHODS: Bone marrow 
      stromal cells (BMSCs) and intragastric administration on ovariectomized(OVX) rats 
      were used to study the anti-osteoporotic function of puerarin. The involvement of 
      mitogen-activated protein kinase (MAPK) signaling pathways was determined. 
      RESULTS: Our results demonstrated that at optimal concentration, puerarin could 
      promote osteogenic differentiation of BMSCs in vitro. This induction was mediated 
      by MAPK signaling pathway. Further detailed study revealed that ERK1/2-Runx2 
      signaling pathway had more prominent effect than p38 signaling pathway in 
      puerarin-induced differentiation of BMSCs toward the osteogenic phenotype. We 
      also found that puerarin protected against reduction in bone mineral density and 
      improved femur trabecular bone structure in ovariectomized rats. CONCLUSION: Our 
      findings revealed the functional mechanism of puerarin in promoting osteogenic 
      differentiation which involved ERK1/2 and p38-MAPK pathway and provided 
      experimental evidence for the potential application of puerarin for estrogen 
      replacement therapy of osteoporosis.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.org.
FAU - Yang, X
AU  - Yang X
AD  - Center of Craniofacial Orthodontics, Department of Oral and Cranio-Maxillofacial 
      Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
FAU - Yang, Y
AU  - Yang Y
AD  - Center of Craniofacial Orthodontics, Department of Oral and Cranio-Maxillofacial 
      Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
FAU - Zhou, S
AU  - Zhou S
AD  - Center of Craniofacial Orthodontics, Department of Oral and Cranio-Maxillofacial 
      Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
FAU - Gong, X
AU  - Gong X
AD  - Center of Craniofacial Orthodontics, Department of Oral and Cranio-Maxillofacial 
      Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
FAU - Dai, Q
AU  - Dai Q
AD  - Center of Craniofacial Orthodontics, Department of Oral and Cranio-Maxillofacial 
      Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
AD  - Department of Pediatric Dentistry, Shanghai 9th People's Hospital, School of 
      Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory of Stomatology & 
      Shanghai Research Institute of Stomatology; National Clinical Research Center of 
      Stomatology, Shanghai 200011, China.
FAU - Zhang, P
AU  - Zhang P
AD  - 2nd Dental Center, Ninth People's Hospital, Shanghai Jiao Tong University School 
      of Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
FAU - Jiang, L
AU  - Jiang L
AD  - Center of Craniofacial Orthodontics, Department of Oral and Cranio-Maxillofacial 
      Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, 639 Zhi Zao Ju Road, Shanghai 200011, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Curr Mol Med
JT  - Current molecular medicine
JID - 101093076
RN  - 0 (Isoflavones)
RN  - 0 (Vasodilator Agents)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects/metabolism
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Isoflavones/*pharmacology
MH  - Mitogen-Activated Protein Kinase 1/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/genetics/*metabolism
MH  - *Osteogenesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Vasodilator Agents/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - MAPKs
OT  - Puerarin
OT  - bone marrow stromal cell
OT  - estrogen replacement therapy.
OT  - osteogenesis
OT  - osteoporosis
EDAT- 2017/12/20 06:00
MHDA- 2019/06/14 06:00
CRDT- 2017/12/20 06:00
PHST- 2017/08/10 00:00 [received]
PHST- 2017/12/12 00:00 [revised]
PHST- 2017/12/14 00:00 [accepted]
PHST- 2017/12/20 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2017/12/20 06:00 [entrez]
AID - CMM-EPUB-87452 [pii]
AID - 10.2174/1566524018666171219101142 [doi]
PST - ppublish
SO  - Curr Mol Med. 2018 Mar 9;17(7):488-496. doi: 10.2174/1566524018666171219101142.