PMID- 29257349 OWN - NLM STAT- MEDLINE DCOM- 20180814 LR - 20211025 IS - 1791-3004 (Electronic) IS - 1791-2997 (Linking) VI - 17 IP - 3 DP - 2018 Mar TI - Competing endogenous RNA network analysis of CD274, IL‑10 and FOXP3 co‑expression in laryngeal squamous cell carcinoma. PG - 3859-3869 LID - 10.3892/mmr.2017.8307 [doi] AB - Laryngeal squamous cell carcinoma (LSCC) is one of the most common types of head and neck malignant tumor; however, there is a lack of effective molecular targets for therapy. The present study detected the expression of three immunity‑associated molecules [forkhead box (FOX)3, interleukin (IL)‑10 and cluster of differentiation (CD)274] in 133 LSCC samples using immunohistochemistry (IHC); subsequently, the association between their expression and the clinical characteristics of LSCC were analyzed. Spearman's rank correlation method, Kaplan‑Meier and Cox regression model were used to analyze the correlations of the three proteins and their clinical significance. StarBase and miRTarBase databases were used to establish the competitive endogenous (ce)RNA network of the three molecules. IHC demonstrated that the positive expression rates of FOXP3, IL‑10 and CD274 were 68.4, 73.7 and 58.6% in 133 LSCC samples, respectively. In addition, it was identified that the expression of the three proteins was closely correlated with the clinical characteristics of LSCC, including lymph node metastasis and prognosis (P<0.05). There was also a significant association of co‑expression between any two proteins (P<0.001). Furthermore, the expression levels of FOXP3, IL‑10 and CD274 were negatively associated with the survival rate of patients with LSCC (P<0.05). The results of a Cox regression model suggested that the three proteins were prognostic risk factors for LSCC (P<0.05). The ceRNA network revealed that 10 microRNAs (miRs; including miR‑16‑5p and miR‑214‑3p), 123 long non‑coding RNAs (including X‑inactive specific transcript, H19 and metastasis associated lung adenocarcinoma transcript 1) and 408 circular RNAs (including ATP‑binding cassette subfamily C member 1 hsa_circ_001569 and ISY1 splicing factor homolog hsa_circ_001859) may regulate the expression of FOXP3, IL‑10 and CD274. The data generated from the present study may increase the understanding of the immune escape mechanisms of LSCC and may be beneficial for the development of a specific immunotherapy. FAU - Sun, Juan AU - Sun J AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Lian, Meng AU - Lian M AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Ma, Hongzhi AU - Ma H AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Wang, Ru AU - Wang R AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Ma, Zhihong AU - Ma Z AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Wang, Haizhou AU - Wang H AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Zhai, Jie AU - Zhai J AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Meng, Lingzhao AU - Meng L AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Feng, Ling AU - Feng L AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. FAU - Bai, Yunfei AU - Bai Y AD - Department of Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, P.R. China. FAU - Cui, Xiaobo AU - Cui X AD - Department of Otorhinolaryngology, Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia 010050, P.R. China. FAU - Fang, Jugao AU - Fang J AD - Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China. LA - eng PT - Journal Article DEP - 20171219 PL - Greece TA - Mol Med Rep JT - Molecular medicine reports JID - 101475259 RN - 0 (B7-H1 Antigen) RN - 0 (CD274 protein, human) RN - 0 (FOXP3 protein, human) RN - 0 (Forkhead Transcription Factors) RN - 0 (IL10 protein, human) RN - 0 (MicroRNAs) RN - 0 (RNA, Circular) RN - 0 (RNA, Long Noncoding) RN - 130068-27-8 (Interleukin-10) RN - 63231-63-0 (RNA) SB - IM MH - Aged MH - B7-H1 Antigen/*genetics/metabolism MH - Carcinoma, Squamous Cell/diagnosis/*genetics/mortality/pathology MH - Female MH - Forkhead Transcription Factors/*genetics/metabolism MH - *Gene Expression Regulation, Neoplastic MH - Humans MH - Interleukin-10/*genetics/metabolism MH - Laryngeal Neoplasms/diagnosis/*genetics/mortality/pathology MH - Lymphatic Metastasis MH - Male MH - MicroRNAs/genetics/metabolism MH - Middle Aged MH - Neoplasm Staging MH - Prognosis MH - RNA/genetics/metabolism MH - RNA, Circular MH - RNA, Long Noncoding/genetics/metabolism MH - Signal Transduction MH - Survival Analysis OTO - NOTNLM OT - laryngeal squamous cell carcinoma OT - forkhead box P3+ regulatory T cells OT - cluster of differentiation 274 OT - interleukin-10 OT - co-expression OT - competitive endogenous RNA network EDAT- 2017/12/20 06:00 MHDA- 2018/08/15 06:00 CRDT- 2017/12/20 06:00 PHST- 2016/08/07 00:00 [received] PHST- 2017/06/15 00:00 [accepted] PHST- 2017/12/20 06:00 [pubmed] PHST- 2018/08/15 06:00 [medline] PHST- 2017/12/20 06:00 [entrez] AID - 10.3892/mmr.2017.8307 [doi] PST - ppublish SO - Mol Med Rep. 2018 Mar;17(3):3859-3869. doi: 10.3892/mmr.2017.8307. Epub 2017 Dec 19.