PMID- 29257654 OWN - NLM STAT- MEDLINE DCOM- 20180219 LR - 20180219 IS - 0254-8704 (Print) IS - 0254-8704 (Linking) VI - 37 IP - 6 DP - 2016 Nov TI - Genetic diversity assessment of Fusarium oxysporum f. sp ciceris isolates of Indian chickpea fields as revealed by the SRAP marker system. PG - 1291-97 AB - An experiment was conducted to study the precise geographical distribution and racial complexity of Fusarium oxysporum f.sp ciceris (Foc) isolates representing 12 states of 4 agro-climatic zones of India at morphological, pathogenic and molecular level. The DNA based sequence related amplified polymorphism (SRAP) markers was employed to differentiate Foc isolates at genome level. The genotypic data output of the isolates was examined for diversity parameter as marker's Polymorphic percentage (PM %), Polymorphic Information Content (PIC), Marker Index (MI) and Gene Diversity Index (DI). As a result, 15 primers used in this study could generated total of 154 reproducible alleles ranging from 100-2100 bp (average allele per marker 10.26) in size, of that 149 (97%) were found to be polymorphic. The neighbor-joining analysis effectively classified the isolates of North East Plain Zone (NEPZ), Central Zone (CZ), North West Plain Zone (NWPZ) and South Zone (SZ) into four clusters. In summary, DNA based marker analysis could differentiate as per isolates geographical location, however pathogenic interaction of isolates from same geographical location could not match the genetic differentiation. Accordingly, considering the present complexity in racial profile, precise classification based on homologs virulence genes specific to races would give a more meaningful in correlating isolates with their native geographical distribution and helps in future resistance breeding programs for sustainable management of vascular wilt disease. FAU - Soren, K R AU - Soren KR FAU - Gangwar, Priyanka AU - Gangwar P FAU - Khatterwani, Payal AU - Khatterwani P FAU - Chaudhary, Ram Ganesh AU - Chaudhary RG FAU - Datta, Subhojit AU - Datta S LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - India TA - J Environ Biol JT - Journal of environmental biology JID - 8300544 RN - 0 (Genetic Markers) SB - IM MH - Cicer/*microbiology MH - Fusarium/genetics MH - *Genetic Markers MH - *Genetic Variation MH - India MH - Nucleic Acid Amplification Techniques MH - Plant Diseases/*microbiology OTO - NOTNLM OT - *Genetic diversity OT - *Focisolate OT - *Pathogenicity OT - *Population structure OT - *Racial profiling OT - *SRAP maker EDAT- 2017/12/20 06:00 MHDA- 2018/02/20 06:00 CRDT- 2017/12/20 06:00 PHST- 2017/12/20 06:00 [entrez] PHST- 2017/12/20 06:00 [pubmed] PHST- 2018/02/20 06:00 [medline] PST - ppublish SO - J Environ Biol. 2016 Nov;37(6):1291-97.