PMID- 29258803 OWN - NLM STAT- MEDLINE DCOM- 20180820 LR - 20220129 IS - 1878-7568 (Electronic) IS - 1742-7061 (Linking) VI - 67 DP - 2018 Feb TI - Antioxidant functionalized polymer capsules to prevent oxidative stress. PG - 21-31 LID - S1742-7061(17)30770-5 [pii] LID - 10.1016/j.actbio.2017.12.014 [doi] AB - Polymeric capsules exhibit significant potential for therapeutic applications as microreactors, where the bio-chemical reactions of interest are efficiently performed in a spatial and time defined manner due to the encapsulation of an active biomolecule (e.g., enzyme) and control over the transfer of reagents and products through the capsular membrane. In this work, catalase loaded polymer capsules functionalized with an external layer of tannic acid (TA) are fabricated via a layer-by-layer approach using calcium carbonate as a sacrificial template. The capsules functionalised with TA exhibit a higher scavenging capacity for hydrogen peroxide and hydroxyl radicals, suggesting that the external layer of TA shows intrinsic antioxidant properties, and represents a valid strategy to increase the overall antioxidant potential of the developed capsules. Additionally, the hydrogen peroxide scavenging capacity of the capsules is enhanced in the presence of the encapsulated catalase. The capsules prevent oxidative stress in an in vitro inflammation model of degenerative disc disease. Moreover, the expression of matrix metalloproteinase-3 (MMP-3), and disintegrin and metalloproteinase with thrombospondin motif-5 (ADAMTS-5), which represents the major proteolytic enzymes in intervertebral disc, are attenuated in the presence of the polymer capsules. This platform technology exhibits potential to reduce oxidative stress, a key modulator in the pathology of a broad range of inflammatory diseases. STATEMENT OF SIGNIFICANCE: Oxidative stress damages important cell structures leading to cellular apoptosis and senescence, for numerous disease pathologies including cancer, neurodegeneration or osteoarthritis. Thus, the development of biomaterials-based systems to control oxidative stress has gained an increasing interest. Herein, polymer capsules loaded with catalase and functionalized with an external layer of tannic acid are fabricated, which can efficiently scavenge important reactive oxygen species (i.e., hydroxyl radicals and hydrogen peroxide) and modulate extracellular matrix activity in an in vitro inflammation model of nucleus pulposus. The present work represents accordingly, an important advance in the development and application of polymer capsules with antioxidant properties for the treatment of oxidative stress, which is applicable for multiple inflammatory disease targets. CI - Copyright (c) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. FAU - Larranaga, Aitor AU - Larranaga A AD - Centre for Research in Medical Devices (CURAM), National University of Ireland, Galway, Ireland; Department of Mining-Metallurgy Engineering and Materials Science & POLYMAT, University of the Basque Country, Bilbao, Spain. FAU - Isa, Isma Liza Mohd AU - Isa ILM AD - Centre for Research in Medical Devices (CURAM), National University of Ireland, Galway, Ireland. FAU - Patil, Vaibhav AU - Patil V AD - Centre for Research in Medical Devices (CURAM), National University of Ireland, Galway, Ireland. FAU - Thamboo, Sagana AU - Thamboo S AD - Chemistry Department, University of Basel, Basel, Switzerland. FAU - Lomora, Mihai AU - Lomora M AD - Centre for Research in Medical Devices (CURAM), National University of Ireland, Galway, Ireland. FAU - Fernandez-Yague, Marc A AU - Fernandez-Yague MA AD - Centre for Research in Medical Devices (CURAM), National University of Ireland, Galway, Ireland. FAU - Sarasua, Jose-Ramon AU - Sarasua JR AD - Department of Mining-Metallurgy Engineering and Materials Science & POLYMAT, University of the Basque Country, Bilbao, Spain. FAU - Palivan, Cornelia G AU - Palivan CG AD - Chemistry Department, University of Basel, Basel, Switzerland. FAU - Pandit, Abhay AU - Pandit A AD - Centre for Research in Medical Devices (CURAM), National University of Ireland, Galway, Ireland. Electronic address: abhay.pandit@nuigalway.ie. LA - eng GR - 174974/SNSF_/Swiss National Science Foundation/Switzerland PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20171216 PL - England TA - Acta Biomater JT - Acta biomaterialia JID - 101233144 RN - 0 (Antioxidants) RN - 0 (Capsules) RN - 0 (Free Radical Scavengers) RN - 0 (Polymers) RN - 0 (Tannins) RN - BBX060AN9V (Hydrogen Peroxide) RN - EC 1.11.1.6 (Catalase) RN - EC 3.4.24.- (ADAMTS5 Protein) RN - EC 3.4.24.17 (Matrix Metalloproteinase 3) SB - IM MH - ADAMTS5 Protein/metabolism MH - Animals MH - Antioxidants/*pharmacology MH - Capsules MH - Catalase/metabolism MH - Cattle MH - Cell Survival/drug effects MH - Free Radical Scavengers/pharmacology MH - Hydrogen Peroxide/toxicity MH - Matrix Metalloproteinase 3/metabolism MH - Nucleus Pulposus/drug effects/metabolism/pathology MH - Oxidative Stress/*drug effects MH - Polymers/*pharmacology MH - Tannins/chemistry OTO - NOTNLM OT - Enzyme encapsulation OT - Intervertebral disc OT - Layer-by-layer OT - Oxidative stress OT - Polymer capsules EDAT- 2017/12/21 06:00 MHDA- 2018/08/21 06:00 CRDT- 2017/12/21 06:00 PHST- 2017/10/03 00:00 [received] PHST- 2017/11/18 00:00 [revised] PHST- 2017/12/11 00:00 [accepted] PHST- 2017/12/21 06:00 [pubmed] PHST- 2018/08/21 06:00 [medline] PHST- 2017/12/21 06:00 [entrez] AID - S1742-7061(17)30770-5 [pii] AID - 10.1016/j.actbio.2017.12.014 [doi] PST - ppublish SO - Acta Biomater. 2018 Feb;67:21-31. doi: 10.1016/j.actbio.2017.12.014. Epub 2017 Dec 16.