PMID- 29259464
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210109
IS  - 1445-5781 (Print)
IS  - 1447-0578 (Electronic)
IS  - 1445-5781 (Linking)
VI  - 16
IP  - 2
DP  - 2017 Apr
TI  - Expression of cytokine-induced neutrophil chemoattractant suppresses tumor 
      necrosis factor alpha expression and thereby prevents the follicles from 
      undergoing atresia and apoptosis.
PG  - 157-165
LID - 10.1002/rmb2.12022 [doi]
AB  - AIM: Cytokine-induced neutrophil chemoattractant (CINC/gro) is a CXC family 
      chemokine, similar to interleukin-8 in rats, and is one of the factors that 
      regulates ovulation. However, the mechanism that regulates atresia of the ovaries 
      postovulation is not clearly defined. METHODS: Whether antibody-blocking of 
      CINC/gro can alter the number of ovulated oocytes and modulate neutrophil 
      infiltration was investigated. The effect of the antibody on the level of 
      inflammatory cytokine production and follicular atresia was examined. Apoptosis 
      was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL) method and via analysis of the messenger RNA expression of Bcl-2 
      and Bcl2-associated X (Bax). RESULTS: The anti-CINC/gro antibody treatment 
      decreased the number of ovulated oocytes. The messenger RNA levels of 
      cyclooxygenase-2 and interleukin-1 beta were decreased by the antibody treatment, 
      whereas that of tumor necrosis factor (TNF) alpha was increased. The TUNEL 
      analysis revealed a larger number of apoptotic cells in the antibody group, 
      compared with those in the control group, as well as a significant increase in 
      the Bax/Bcl-2 ratio 24 hours after human chorionic gonadotropin administration. 
      CONCLUSION: These findings suggest that ovulation is accelerated by neutrophil 
      infiltration into the theca layer. The CINC/gro appears to synergize with 
      interleukin-1 beta for ovulation. By contrast, the data suggest that CINC/gro 
      expression suppresses TNF alpha expression and that CINC/gro expression therefore 
      prevents the follicles from undergoing atresia and apoptosis.
FAU - Tanaka, Yu
AU  - Tanaka Y
AD  - Department of Obstetrics and Gynecology Japanese Red Cross Kochi Hospital Kochi 
      Japan.
FAU - Kuwahara, Akira
AU  - Kuwahara A
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
FAU - Ushigoe, Kenjiro
AU  - Ushigoe K
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
FAU - Yano, Yuya
AU  - Yano Y
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
FAU - Taniguchi, Yuka
AU  - Taniguchi Y
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
FAU - Yamamoto, Yuri
AU  - Yamamoto Y
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
FAU - Matsuzaki, Toshiya
AU  - Matsuzaki T
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
FAU - Yasui, Toshiyuki
AU  - Yasui T
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
FAU - Irahara, Minoru
AU  - Irahara M
AD  - Department of Obstetrics and Gynecology Institute of Health Biosciences The 
      University of Tokushima Graduate School Tokushima Japan.
LA  - eng
PT  - Journal Article
DEP - 20170312
PL  - Japan
TA  - Reprod Med Biol
JT  - Reproductive medicine and biology
JID - 101213278
PMC - PMC5661809
OTO - NOTNLM
OT  - apoptosis
OT  - follicle
OT  - ovary
OT  - ovulation
OT  - tumor necrosis factor alpha
EDAT- 2017/12/21 06:00
MHDA- 2017/12/21 06:01
PMCR- 2017/03/12
CRDT- 2017/12/21 06:00
PHST- 2016/10/18 00:00 [received]
PHST- 2017/01/04 00:00 [accepted]
PHST- 2017/12/21 06:00 [entrez]
PHST- 2017/12/21 06:00 [pubmed]
PHST- 2017/12/21 06:01 [medline]
PHST- 2017/03/12 00:00 [pmc-release]
AID - RMB212022 [pii]
AID - 10.1002/rmb2.12022 [doi]
PST - epublish
SO  - Reprod Med Biol. 2017 Mar 12;16(2):157-165. doi: 10.1002/rmb2.12022. eCollection 
      2017 Apr.