PMID- 29259620
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240213
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 8
DP  - 2017
TI  - HLA-DR Genotyping and Mitochondrial DNA Analysis Reveal the Presence of Family 
      Burials in a Fourth Century Romano-British Christian Cemetery.
PG  - 182
LID - 10.3389/fgene.2017.00182 [doi]
LID - 182
AB  - In Colchester, Britain's oldest recorded town, during the Roman period there were 
      areas which were clearly used solely as cemeteries. One of the most significant 
      is at Butt Road, which includes a late Roman probable Christian cemetery with an 
      associated building, apparently a church, that overlies and developed from a 
      pagan inhumation cemetery. DNA was extracted from the long bones (femurs) of 29 
      individuals, mostly from a large complex of burials centered on two timber 
      vaults. These were thought to comprise a number of family groupings, deduced from 
      osteological analysis, stratigraphical and other considerations. The use of a 
      modified version of the silica-based purification method recovered nanogram 
      quantities of DNA/gram of bone. Two-stage amplification, incorporating 
      primer-extension preamplification-polymerase chain reaction, permitted 
      simultaneous amplification of both mitochondrial and nuclear DNA. 
      Sequence-specific oligonucleotide probes yielded human leukocyte antigen (HLA)-DR 
      typing of seven samples, with four revealing the infrequent HLA-DR10 genotype. 
      Examination of the control region of mitochondrial DNA (mtDNA) by direct 
      sequencing revealed polymorphisms yet to be reported in the modern population. 
      HLA-DRB typing and mtDNA analysis affirmatively supported kinship among some, if 
      not all, individuals in the "vault complex" and demonstrate a continental 
      European origin of the individuals investigated.
FAU - Voong, Canh P
AU  - Voong CP
AD  - School of Biological Sciences, University of Essex, Colchester, United Kingdom.
FAU - Spencer, Patrick S
AU  - Spencer PS
AD  - School of Biological Sciences, University of Essex, Colchester, United Kingdom.
FAU - Navarrete, Cristina V
AU  - Navarrete CV
AD  - Histocompatibility and Immunogenetics Laboratory, NHS Blood and Transplant, 
      London, United Kingdom.
AD  - Division of Infection and Immunity, Faculty of Medical Sciences, University 
      College London, London, United Kingdom.
FAU - Turner, David
AU  - Turner D
AD  - Histocompatibility and Immunogenetics Laboratory, Scottish National Blood 
      Transfusion Service, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom.
FAU - Hayrabedyan, Soren B
AU  - Hayrabedyan SB
AD  - Institute of Biology and Immunology of Reproduction, "Akad. Kiril Bratanov," 
      Bulgarian Academy of Sciences (BAS), Sofia, Bulgaria.
FAU - Crummy, Philip
AU  - Crummy P
AD  - Colchester Archaeological Trust, Colchester, United Kingdom.
FAU - Holloway, Emma
AU  - Holloway E
AD  - Colchester Archaeological Trust, Colchester, United Kingdom.
FAU - Wilson, Mike T
AU  - Wilson MT
AD  - School of Biological Sciences, University of Essex, Colchester, United Kingdom.
FAU - Smith, Patricia R
AU  - Smith PR
AD  - MAPI Group, Ontario, Canada.
FAU - Fernandez, Nelson
AU  - Fernandez N
AD  - School of Biological Sciences, University of Essex, Colchester, United Kingdom.
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
DEP - 20171205
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC5723391
OTO - NOTNLM
OT  - Colchester (Camulodunum)
OT  - HLA-DR
OT  - extramural fourth century Romano-British cemetery
OT  - family groupings
OT  - mtDNA
EDAT- 2017/12/21 06:00
MHDA- 2017/12/21 06:01
PMCR- 2017/12/05
CRDT- 2017/12/21 06:00
PHST- 2017/07/29 00:00 [received]
PHST- 2017/11/06 00:00 [accepted]
PHST- 2017/12/21 06:00 [entrez]
PHST- 2017/12/21 06:00 [pubmed]
PHST- 2017/12/21 06:01 [medline]
PHST- 2017/12/05 00:00 [pmc-release]
AID - 10.3389/fgene.2017.00182 [doi]
PST - epublish
SO  - Front Genet. 2017 Dec 5;8:182. doi: 10.3389/fgene.2017.00182. eCollection 2017.