PMID- 29259771
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 2046-1402 (Print)
IS  - 2046-1402 (Electronic)
IS  - 2046-1402 (Linking)
VI  - 6
DP  - 2017
TI  - Phenotype and genotype of  lactic acid bacteria (LAB) isolated from the tiger 
      grouper Epinephelus fuscoguttatus alimentary tract.
PG  - 1984
LID - 10.12688/f1000research.12734.1 [doi]
LID - 1984
AB  - Lactic acid bacteria (LAB) have been isolated successfully from the tiger grouper 
      Epinephelusfuscoguttatus intestine. However, their genus or species have not been 
      identified. Therefore, the objective of the present study was to characterize the 
      three isolated LAB (KSBU-12C, KSBU-5Da, and KSBU-9) based on their phenotype and 
      genotype. The LAB phenotype was examined by observing morphological features 
      including cell morphology, spore production and motility. The physiological tests 
      were performed in 6.5% NaCl at the  temperatures of 10 (o)C and 45 (o)C, and the 
      biochemical tests were evaluated based on the production of enzymes catalase, 
      oxidase and arginine dehydrolase, following  the Standard Analytical Profile 
      Index, API 50 CH kit.  The genotype was examined based on 16S rDNA gene sequence 
      analysis , and the products were analyzed using the BLAST (Basic Local Alignment 
      Search Tool) NCBI database. The three isolates (KSBU-5Da, KSBU-12C, and KSBU-9) 
      were categorized into the genus Enterococcus. 16S rDNA sequence analysis 
      indicated that the isolates had 99% similarity to E. hirae ATCC 9790, registered 
      in GenBank with accession number NR_075022.1. It was concluded that the three LAB 
      isolates taken from the tiger grouper Epinephelus fuscoguttatus are E. hirae.
FAU - Nursyirwani, Nursyirwani
AU  - Nursyirwani N
AD  - Faculty of Fishery and Marine Science, Universitas Riau, Pekanbaru, Indonesia.
FAU - Asmara, Widya
AU  - Asmara W
AD  - Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
FAU - Wahyuni, Agnesia Endang Tri Hastuti
AU  - Wahyuni AETH
AD  - Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
FAU - Triyanto, Triyanto
AU  - Triyanto T
AD  - Department of Fisheries, Faculty of Agriculture, Gadjah Mada University, 
      Yogyakarta, Indonesia.
FAU - Fauzi, Muhammad
AU  - Fauzi M
AUID- ORCID: 0000-0002-9159-155X
AD  - Faculty of Fishery and Marine Science, Universitas Riau, Pekanbaru, Indonesia.
FAU - Muchlisin, Zainal Abidin
AU  - Muchlisin ZA
AUID- ORCID: 0000-0002-0858-1853
AD  - Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, Indonesia.
LA  - eng
PT  - Journal Article
DEP - 20171110
PL  - England
TA  - F1000Res
JT  - F1000Research
JID - 101594320
PMC - PMC5717476
OTO - NOTNLM
OT  - characterization
OT  - genotype
OT  - lactic acid bacteria
OT  - phenotype
OT  - tiger grouper
COIS- No competing interests were disclosed.
EDAT- 2017/12/21 06:00
MHDA- 2017/12/21 06:01
PMCR- 2017/11/10
CRDT- 2017/12/21 06:00
PHST- 2017/11/08 00:00 [accepted]
PHST- 2017/12/21 06:00 [entrez]
PHST- 2017/12/21 06:00 [pubmed]
PHST- 2017/12/21 06:01 [medline]
PHST- 2017/11/10 00:00 [pmc-release]
AID - 10.12688/f1000research.12734.1 [doi]
PST - epublish
SO  - F1000Res. 2017 Nov 10;6:1984. doi: 10.12688/f1000research.12734.1. eCollection 
      2017.