PMID- 29260555
OWN - NLM
STAT- MEDLINE
DCOM- 20190201
LR  - 20190201
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 90
IP  - 2
DP  - 2018 Jan 16
TI  - Investigation of Silver Nanoparticle Induced Lipids Changes on a Single Cell 
      Surface by Time-of-Flight Secondary Ion Mass Spectrometry.
PG  - 1072-1076
LID - 10.1021/acs.analchem.7b04591 [doi]
AB  - Lipids are the main component of the cell membrane. They not only provide 
      structural support of cells but also directly participate in complex cellular 
      metabolic processes. Lipid signaling is an important part of cell signaling. 
      Evidence showed that abnormal cellular metabolism may induce lipids changes. 
      Besides, owing to single cell heterogeneity, it is necessary to distinguish 
      different behaviors of individual cells. Time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS) is a sensitive surface analysis technique with high 
      spatial resolution, which is useful in single cell surface analysis. Herein, we 
      used ToF-SIMS to investigate silver nanoparticle induced lipids changes on the 
      surface of single macrophage cells. Delayed extraction mode of ToF-SIMS was used 
      to simultaneously obtain high mass resolution of mass spectra and high spatial 
      resolution of single cell chemical imaging. Principle component analysis (PCA) 
      results showed good agreement with the cytotoxicity assay results. Clear 
      distinctions were observed between the cell groups treated with high or low dose 
      of silver nanoparticles. The loadings plots revealed that the separation was 
      mainly due to changes of cholesterol and diacylglycerol (DAG) as well as 
      monoacylglycerol (MAG). Meanwhile, the chemical mapping of single cell components 
      showed that cholesterol and DAG tend to migrate to the surrounding of the cells 
      after high dose silver nanoparticles (Ag NPs) treatment. Our results demonstrated 
      the feasibility of ToF-SIMS for characterizing the changes of the lipids on a 
      single cell surface, providing a better understanding of the mechanism of 
      cell-nanoparticle interactions at the molecular level.
FAU - Hua, Xin
AU  - Hua X
AD  - Key Laboratory of Advanced Materials, School of Chemistry and Molecular 
      Engineering, East China University of Science and Technology , Shanghai, 200237, 
      P. R. China.
FAU - Li, Hao-Wen
AU  - Li HW
AD  - Key Laboratory of Advanced Materials, School of Chemistry and Molecular 
      Engineering, East China University of Science and Technology , Shanghai, 200237, 
      P. R. China.
FAU - Long, Yi-Tao
AU  - Long YT
AUID- ORCID: 0000-0003-2571-7457
AD  - Key Laboratory of Advanced Materials, School of Chemistry and Molecular 
      Engineering, East China University of Science and Technology , Shanghai, 200237, 
      P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171226
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Diglycerides)
RN  - 0 (Lipids)
RN  - 0 (Monoglycerides)
RN  - 3M4G523W1G (Silver)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Cholesterol/analysis/metabolism
MH  - Diglycerides/analysis/metabolism
MH  - *Lipid Metabolism
MH  - Lipids/analysis
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Monoglycerides/analysis/metabolism
MH  - Nanoparticles/*metabolism
MH  - RAW 264.7 Cells
MH  - Silver/*metabolism
MH  - Single-Cell Analysis/*methods
MH  - Spectrometry, Mass, Secondary Ion/*methods
EDAT- 2017/12/21 06:00
MHDA- 2019/02/02 06:00
CRDT- 2017/12/21 06:00
PHST- 2017/12/21 06:00 [pubmed]
PHST- 2019/02/02 06:00 [medline]
PHST- 2017/12/21 06:00 [entrez]
AID - 10.1021/acs.analchem.7b04591 [doi]
PST - ppublish
SO  - Anal Chem. 2018 Jan 16;90(2):1072-1076. doi: 10.1021/acs.analchem.7b04591. Epub 
      2017 Dec 26.