PMID- 29260891
OWN - NLM
STAT- MEDLINE
DCOM- 20180810
LR  - 20180810
IS  - 1205-7541 (Electronic)
IS  - 0008-4212 (Linking)
VI  - 96
IP  - 2
DP  - 2018 Feb
TI  - TNF-alpha antagonism with etanercept enhances penile NOS expression, cavernosal 
      reactivity, and testosterone levels in aged rats.
PG  - 200-207
LID - 10.1139/cjpp-2017-0113 [doi]
AB  - Erectile dysfunction (ED) has been reported to be associated with inflammation. 
      This study investigated the effects of tumor necrosis factor alpha (TNF-alpha) 
      inhibitor etanercept on penile neuronal nitric oxide synthase (nNOS) and 
      endothelial nitric oxide synthase (eNOS) expressions, testosterone 
      concentrations, neurogenic and endothelium-dependent relaxations of corpus 
      cavernosum (CC), and circulating and cavernosal levels of inflammatory markers in 
      aged rats. Animals were separated into control, aged, and etanercept-treated aged 
      groups. Aged rats displayed significantly increased serum and cavernosal TNF-alpha, 
      C-reactive protein (CRP), monocyte chemoattractant protein-1 (MCP-1) and 
      intercellular adhesion molecule (ICAM-1) levels, and decreased penile nNOS and 
      eNOS expressions and serum testosterone levels compared with controls. In 
      etanercept-treated aged group, NOS expressions were similar to that of the 
      control group. The circulating and cavernosal concentrations of TNF-alpha, CRP, 
      MCP-1, ICAM-1, and testosterone were also normalized by etanercept. Neurogenic 
      and endothelium-dependent relaxant responses significantly decreased in aged rats 
      and etanercept treatment markedly improved these relaxation responses. Our 
      findings indicate that aging decreases penile NOS expression, neurogenic and 
      endothelium-dependent relaxations of CC, and also suppresses serum testosterone 
      levels by inducing inflammatory response that may contribute to the development 
      of ED. TNF-alpha antagonism may be a novel strategy to treat aging-associated ED.
FAU - Demirtas Sahin, Tugce
AU  - Demirtas Sahin T
AD  - a Department of Pharmacology, Medical Faculty, Kocaeli University, Kocaeli 41380, 
      Turkey.
FAU - Yazir, Yusufhan
AU  - Yazir Y
AD  - b Department of Histology and Embryology, Medical Faculty, Kocaeli University, 
      Kocaeli 41380, Turkey.
AD  - c Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, 
      Kocaeli 41380, Turkey.
FAU - Utkan, Tijen
AU  - Utkan T
AD  - a Department of Pharmacology, Medical Faculty, Kocaeli University, Kocaeli 41380, 
      Turkey.
AD  - d Experimental Medical Research and Application Center, Kocaeli University, 
      Kocaeli 41380, Turkey.
FAU - Gacar, Gulcin
AU  - Gacar G
AD  - c Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, 
      Kocaeli 41380, Turkey.
FAU - Furat Rencber, Selenay
AU  - Furat Rencber S
AD  - b Department of Histology and Embryology, Medical Faculty, Kocaeli University, 
      Kocaeli 41380, Turkey.
FAU - Gocmez, Semil Selcen
AU  - Gocmez SS
AD  - a Department of Pharmacology, Medical Faculty, Kocaeli University, Kocaeli 41380, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20171220
PL  - Canada
TA  - Can J Physiol Pharmacol
JT  - Canadian journal of physiology and pharmacology
JID - 0372712
RN  - 0 (Biomarkers)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3XMK78S47O (Testosterone)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - OP401G7OJC (Etanercept)
SB  - IM
MH  - Aging/*blood
MH  - Animals
MH  - Biomarkers/blood
MH  - Body Weight/drug effects
MH  - Carbachol/pharmacology
MH  - Electric Stimulation
MH  - Endothelium, Vascular/drug effects/metabolism
MH  - Etanercept/*pharmacology
MH  - Inflammation/blood/pathology
MH  - Male
MH  - Nitric Oxide Synthase Type I/*metabolism
MH  - Nitric Oxide Synthase Type III/*metabolism
MH  - Organ Size/drug effects
MH  - Penis/*enzymology
MH  - Rats, Wistar
MH  - Testosterone/*blood
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors/metabolism
MH  - Vasodilation/drug effects
OTO - NOTNLM
OT  - TNF-alpha
OT  - aging
OT  - dysfonction erectile
OT  - eNOS
OT  - erectile dysfunction
OT  - etanercept
OT  - inflammation
OT  - nNOS
OT  - vieillissement
OT  - etanercept
EDAT- 2017/12/21 06:00
MHDA- 2018/08/11 06:00
CRDT- 2017/12/21 06:00
PHST- 2017/12/21 06:00 [pubmed]
PHST- 2018/08/11 06:00 [medline]
PHST- 2017/12/21 06:00 [entrez]
AID - 10.1139/cjpp-2017-0113 [doi]
PST - ppublish
SO  - Can J Physiol Pharmacol. 2018 Feb;96(2):200-207. doi: 10.1139/cjpp-2017-0113. 
      Epub 2017 Dec 20.