PMID- 29262412
OWN - NLM
STAT- MEDLINE
DCOM- 20180312
LR  - 20180312
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 153
IP  - 3
DP  - 2017
TI  - Integrated Karyotyping of Woodland Strawberry (Fragaria vesca) with Oligopaint 
      FISH Probes.
PG  - 158-164
LID - 10.1159/000485283 [doi]
AB  - Chromosome identification is critical for many aspects of cytogenetic research. 
      However, for Fragaria vesca, definite identification of individual chromosomes is 
      almost impossible because of their small size and high similarity. Here, we 
      demonstrate that bulked oligonucleotide (oligo) probes can be used as 
      chromosome-specific DNA markers for chromosome identification in F. vesca. Oligos 
      specific to entire pseudochromosomes in the draft genome of F. vesca were 
      identified and synthesized as libraries. In all, we synthesized 6 oligo libraries 
      corresponding to 6 pseudochromosomes of F. vesca. These libraries were amplified 
      and labeled as probes for fluorescence in situ hybridization (FISH). Two rounds 
      of multicolor FISH analysis were sequentially conducted on the same metaphase 
      cells with each round including 3 probe libraries, which permitted simultaneous 
      identification of all chromosomes of F. vesca. Moreover, 45S and 5S rDNA were 
      mapped to chromosomes 1, 2, and 7, respectively. A karyotype of metaphase 
      chromosomes was constructed, representing the first FISH-based molecular 
      cytogenetic karyotype of F. vesca. Our study can serve as a basis for future 
      comparative cytogenetic research through cross-species chromosome painting using 
      bulked oligo probes and will facilitate the application of breeding technologies 
      that rely on the identification of chromosomes in the genus Fragaria.
CI  - (c) 2017 S. Karger AG, Basel.
FAU - Qu, Manman
AU  - Qu M
AD  - Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life 
      Sciences, Jiangsu Normal University, Xuzhou, China.
FAU - Li, Kunpeng
AU  - Li K
FAU - Han, Yanli
AU  - Han Y
FAU - Chen, Lei
AU  - Chen L
FAU - Li, Zongyun
AU  - Li Z
FAU - Han, Yonghua
AU  - Han Y
LA  - eng
PT  - Journal Article
DEP - 20171221
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, ribosomal, 45S)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Plant/*genetics
MH  - DNA Probes
MH  - Fragaria/*genetics
MH  - Karyotype
MH  - Karyotyping/*methods
MH  - Plant Breeding/methods
MH  - RNA, Ribosomal/genetics
MH  - RNA, Ribosomal, 5S/genetics
OTO - NOTNLM
OT  - <italic>Fragaria vesca</italic>
OT  - FISH
OT  - Karyotype
OT  - Oligonucleotide probes
OT  - rDNA
EDAT- 2017/12/21 06:00
MHDA- 2018/03/13 06:00
CRDT- 2017/12/21 06:00
PHST- 2017/09/15 00:00 [accepted]
PHST- 2017/12/21 06:00 [pubmed]
PHST- 2018/03/13 06:00 [medline]
PHST- 2017/12/21 06:00 [entrez]
AID - 000485283 [pii]
AID - 10.1159/000485283 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2017;153(3):158-164. doi: 10.1159/000485283. Epub 2017 Dec 
      21.