PMID- 29262879
OWN - NLM
STAT- MEDLINE
DCOM- 20190410
LR  - 20190410
IS  - 1469-8161 (Electronic)
IS  - 0031-1820 (Linking)
VI  - 145
IP  - 8
DP  - 2018 Jul
TI  - The role of the mussel Mytilus spp. in the transmission of ostreid herpesvirus-1 
      microVar.
PG  - 1095-1104
LID - 10.1017/S0031182017002244 [doi]
AB  - The Pacific oyster Crassostrea gigas contributes significantly to global 
      aquaculture; however, C. gigas culture has been affected by ostreid herpesvirus-1 
      (OsHV-1) and variants. The dynamics of how the virus maintains itself at culture 
      sites is unclear and the role of carriers, reservoirs or hosts is unknown. Both 
      wild and cultured mussels Mytilus spp. (Mytilus edulis, Mytilus galloprovincialis 
      and hybrids) are commonly found at C. gigas culture sites. The objective of this 
      study was to investigate if Mytilus spp. can harbour the virus and if viral 
      transmission can occur between mussels and oysters. Mytilus spp. living at oyster 
      trestles, 400-500 m higher up the shore from the trestles and up to 26 km at 
      non-culture sites were screened for OsHV-1 and variants by all the World 
      Organization for Animal Health (OIE) recommended diagnostic methods including 
      polymerase chain reaction (PCR), quantitative PCR (qPCR), histology, in situ 
      hybridization and confirmation using direct sequencing. The particular primers 
      that target OsHV-1 and variants, including OsHV-1 microVar (muVar), were used in 
      the PCR and qPCR. OsHV-1 muVar was detected in wild Mytilus spp. at C. gigas 
      culture sites and more significantly the virus was detected in mussels at 
      non-culture sites. Cohabitation of exposed wild mussels and naive C. gigas 
      resulted in viral transmission after 14 days, under an elevated temperature 
      regime. These results indicate that mussels can harbour OsHV-1 muVar; however, the 
      impact of OsHV-1 muVar on Mytilus spp. requires further investigation.
FAU - O' Reilly, A J
AU  - O' Reilly AJ
AD  - Aquaculture and Fisheries Development Centre,School of Biological,Earth and 
      Environmental Sciences,University College Cork,The Cooperage,Distillery 
      Fields,North Mall,Cork,Ireland.
FAU - Laide, C
AU  - Laide C
AD  - Aquaculture and Fisheries Development Centre,School of Biological,Earth and 
      Environmental Sciences,University College Cork,The Cooperage,Distillery 
      Fields,North Mall,Cork,Ireland.
FAU - Maloy, A
AU  - Maloy A
AD  - U.S. Fish & Wildlife Service,Northeast Fishery Centre,PO Box 75,227 Washington 
      Ave Lamar PA,16848,USA.
FAU - Hutton, S
AU  - Hutton S
AD  - Aquaculture and Fisheries Development Centre,School of Biological,Earth and 
      Environmental Sciences,University College Cork,The Cooperage,Distillery 
      Fields,North Mall,Cork,Ireland.
FAU - Bookelaar, B
AU  - Bookelaar B
AD  - Aquaculture and Fisheries Development Centre,School of Biological,Earth and 
      Environmental Sciences,University College Cork,The Cooperage,Distillery 
      Fields,North Mall,Cork,Ireland.
FAU - O' Sullivan, K
AU  - O' Sullivan K
AD  - School of Mathematical Sciences,Room 1.58,Western Gateway Building,University 
      College Cork,Cork,Ireland.
FAU - Lynch, S A
AU  - Lynch SA
AD  - Aquaculture and Fisheries Development Centre,School of Biological,Earth and 
      Environmental Sciences,University College Cork,The Cooperage,Distillery 
      Fields,North Mall,Cork,Ireland.
FAU - Culloty, S C
AU  - Culloty SC
AD  - Aquaculture and Fisheries Development Centre,School of Biological,Earth and 
      Environmental Sciences,University College Cork,The Cooperage,Distillery 
      Fields,North Mall,Cork,Ireland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171221
PL  - England
TA  - Parasitology
JT  - Parasitology
JID - 0401121
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - Crassostrea/*virology
MH  - DNA Primers
MH  - DNA Viruses/genetics/*isolation & purification
MH  - DNA, Viral
MH  - Disease Reservoirs/virology
MH  - Herpesviridae Infections/transmission/*veterinary
MH  - Mytilus/*virology
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Cohabitation
OT  - Crassostrea gigas
OT  - Herpesvirus
OT  - Mytilus spp.
OT  - interspecies transmission
OT  - temperature
EDAT- 2017/12/22 06:00
MHDA- 2019/04/11 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2019/04/11 06:00 [medline]
PHST- 2017/12/22 06:00 [entrez]
AID - S0031182017002244 [pii]
AID - 10.1017/S0031182017002244 [doi]
PST - ppublish
SO  - Parasitology. 2018 Jul;145(8):1095-1104. doi: 10.1017/S0031182017002244. Epub 
      2017 Dec 21.