PMID- 29263199
OWN - NLM
STAT- MEDLINE
DCOM- 20190205
LR  - 20200930
IS  - 1469-3178 (Electronic)
IS  - 1469-221X (Print)
IS  - 1469-221X (Linking)
VI  - 19
IP  - 3
DP  - 2018 Mar
TI  - The dynamic recruitment of TRBP to neuronal membranes mediates dendritogenesis 
      during development.
LID - 10.15252/embr.201744853 [doi]
LID - e44853
AB  - MicroRNAs are important regulators of local protein synthesis during neuronal 
      development. We investigated the dynamic regulation of microRNA production and 
      found that the majority of the microRNA-generating complex, consisting of Dicer, 
      TRBP, and PACT, specifically associates with intracellular membranes in 
      developing neurons. Stimulation with brain-derived neurotrophic factor (BDNF), 
      which promotes dendritogenesis, caused the redistribution of TRBP from the 
      endoplasmic reticulum into the cytoplasm, and its dissociation from Dicer, in a 
      Ca(2+)-dependent manner. As a result, the processing of a subset of neuronal 
      precursor microRNAs, among them the dendritically localized pre-miR16, was 
      impaired. Decreased production of miR-16-5p, which targeted the BDNF mRNA itself, 
      was rescued by expression of a membrane-targeted TRBP Moreover, miR-16-5p or 
      membrane-targeted TRBP expression blocked BDNF-induced dendritogenesis, 
      demonstrating the importance of neuronal TRBP dynamics for activity-dependent 
      neuronal development. We propose that neurons employ specialized mechanisms to 
      modulate local gene expression in dendrites, via the dynamic regulation of 
      microRNA biogenesis factors at intracellular membranes of the endoplasmic 
      reticulum, which in turn is crucial for neuronal dendrite complexity and 
      therefore neuronal circuit formation and function.
CI  - (c) 2017 The Authors. Published under the terms of the CC BY 4.0 license.
FAU - Antoniou, Anna
AU  - Antoniou A
AUID- ORCID: 0000-0002-3907-2330
AD  - Institute for Physiological Chemistry, Biochemical-Pharmacological Center 
      Marburg, Philipps-University of Marburg, Marburg, Germany 
      anna.antoniou@staff.uni-marburg.de gerhard.schratt@hest.ethz.ch.
FAU - Khudayberdiev, Sharof
AU  - Khudayberdiev S
AD  - Institute for Physiological Chemistry, Biochemical-Pharmacological Center 
      Marburg, Philipps-University of Marburg, Marburg, Germany.
FAU - Idziak, Agata
AU  - Idziak A
AD  - Institute for Physiological Chemistry, Biochemical-Pharmacological Center 
      Marburg, Philipps-University of Marburg, Marburg, Germany.
FAU - Bicker, Silvia
AU  - Bicker S
AUID- ORCID: 0000-0002-6276-5653
AD  - Institute for Physiological Chemistry, Biochemical-Pharmacological Center 
      Marburg, Philipps-University of Marburg, Marburg, Germany.
FAU - Jacob, Ralf
AU  - Jacob R
AD  - Department of Cell Biology and Cell Pathology, Philipps-University of Marburg, 
      Marburg, Germany.
FAU - Schratt, Gerhard
AU  - Schratt G
AUID- ORCID: 0000-0001-7527-2025
AD  - Institute for Physiological Chemistry, Biochemical-Pharmacological Center 
      Marburg, Philipps-University of Marburg, Marburg, Germany 
      anna.antoniou@staff.uni-marburg.de gerhard.schratt@hest.ethz.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171220
PL  - England
TA  - EMBO Rep
JT  - EMBO reports
JID - 100963049
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN16 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Ncoa6 protein, rat)
RN  - 0 (Nuclear Receptor Coactivators)
RN  - 0 (RNA-Binding Proteins)
RN  - EC 3.1.26.3 (Dicer1 protein, rat)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
CIN - EMBO Rep. 2018 Mar;19(3):. PMID: 29459484
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - DEAD-box RNA Helicases/genetics
MH  - Dendrites/*genetics
MH  - Embryo, Mammalian
MH  - Embryonic Development/genetics
MH  - Endoplasmic Reticulum/genetics/metabolism
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - Neurogenesis/*genetics
MH  - Neurons/metabolism
MH  - Nuclear Receptor Coactivators/*genetics
MH  - RNA-Binding Proteins/genetics
MH  - Rats
MH  - Ribonuclease III/genetics
PMC - PMC5835843
OTO - NOTNLM
OT  - BDNF-induced dendritogenesis
OT  - endoplasmic reticulum
OT  - isomiR
OT  - miR-16
OT  - pre-miRNA processing
EDAT- 2017/12/22 06:00
MHDA- 2019/02/06 06:00
PMCR- 2017/12/20
CRDT- 2017/12/22 06:00
PHST- 2017/07/18 00:00 [received]
PHST- 2017/11/22 00:00 [revised]
PHST- 2017/11/27 00:00 [accepted]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2019/02/06 06:00 [medline]
PHST- 2017/12/22 06:00 [entrez]
PHST- 2017/12/20 00:00 [pmc-release]
AID - embr.201744853 [pii]
AID - EMBR201744853 [pii]
AID - 10.15252/embr.201744853 [doi]
PST - ppublish
SO  - EMBO Rep. 2018 Mar;19(3):e44853. doi: 10.15252/embr.201744853. Epub 2017 Dec 20.