PMID- 29263461
OWN - NLM
STAT- MEDLINE
DCOM- 20180725
LR  - 20181202
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 49
IP  - 6
DP  - 2017 Dec 18
TI  - [Mechanism of cross talk between tissue factor/active coagulation factor VII and 
      epidermal growth factor receptor signalings in colon cancer cells in culture].
PG  - 931-936
AB  - OBJECTIVE: To preliminarily verify the cross talk between tissue factor/active 
      coagulation factor VII (TF/FVIIa) and epidermal growth factor receptor (EGFR) 
      pathways in human colon cancer cells in culture. METHODS: FVIIa was treated to 
      HT-29 (KRAS-wild type) and LoVo (KRAS-mutant) colon cancer cells to activate 
      TF/FVIIa pathway, qRT-PCR and Western blot were used to detect the expressions of 
      amphiregulin (AREG) and epiregulin (EREG), ligands of EGFR on mRNA and protein 
      levels, respectively. After knocking down expression of TF by TF-targeted siRNA 
      transfection, FVIIa was treated and mRNA expressions of AREG and EREG were 
      detected to see whether the FVIIa-induced effects were dependent on TF. 
      Expressions of mRNA of TF and FVII were detected by qRT-PCR following the 
      activation of EGFR pathway by treatment with epidermal growth factor (EGF) to 
      HT-29 and LoVo cells. RESULTS: After TF/FVIIa pathway was activated, for HT-29 
      cells, expressions of AREG (on mRNA level) and EREG (both on mRNA and protein 
      level) were significantly down-regulated versus those of control group, gene 
      expressions of AREG and EREG were 0.55+/-0.09 vs.0.99 +/-0.09, 0.67+/-0.10 
      vs.1.02+/-0.02, protein expressions of EREG were 0.54+/-0.09 vs.1.04+/-0.13, all 
      P<0.05. For LoVo cells, expressions of AREG (both on mRNA and protein level) and 
      EREG (on protein level) were significantly up-regulated versus those of control 
      group, gene expression of AREG were 1.87+/-0.39 vs. 0.93+/-0.23, protein expressions 
      of AREG and EREG were 3.09+/-0.73 vs.1.11+/-0.21, 1.53+/-0.19 vs.0.97+/-0.23, all P<0.05. 
      The regulating effect of AREG and EREG mRNA expression by FVIIa in HT-29 and LoVo 
      cells could both be partly blocked by knocking down TF expression. For HT-29 
      cells, activation of EGFR pathway induced no significant TF mRNA expression, FVII 
      mRNA expression was not detected. However,for LoVo cells, activation of EGFR 
      pathway induced significantly higher mRNA expressions of both TF and FVII, 
      expressions were 1.53+/-0.23 vs.1.00+/-0.23, 53.20+/-6.08 vs.1.00+/-0.15, all P<0.05. 
      CONCLUSION: In colon cancer cell LoVo, when activated, TF/FVIIa pathway and EGFR 
      pathway could interact through upregulating the other pathway's effectors, and 
      mutant KRAS might play a critical role in the two pathways' cross talk.
FAU - Chen, H K
AU  - Chen HK
AD  - Department of General Surgery, Peking University First Hospital, Beijing 100034, 
      China.
FAU - Dai, Y
AU  - Dai Y
AD  - Department of Gastroenterology, Peking University First Hospital, Beijing 100034, 
      China.
FAU - Wu, T
AU  - Wu T
AD  - Department of Gastroenterology, Peking University First Hospital, Beijing 100034, 
      China.
FAU - Wang, X
AU  - Wang X
AD  - Department of General Surgery, Peking University First Hospital, Beijing 100034, 
      China.
FAU - Wan, Y L
AU  - Wan YL
AD  - Department of General Surgery, Peking University First Hospital, Beijing 100034, 
      China.
FAU - Tang, J Q
AU  - Tang JQ
AD  - Department of General Surgery, Peking University First Hospital, Beijing 100034, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
RN  - 0 (Amphiregulin)
RN  - 0 (EREG protein, human)
RN  - 0 (Epiregulin)
RN  - 0 (RNA, Messenger)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9001-25-6 (Factor VII)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amphiregulin/physiology
MH  - Cell Count
MH  - Colonic Neoplasms/*metabolism
MH  - Epidermal Growth Factor
MH  - Epiregulin
MH  - ErbB Receptors/*physiology
MH  - Factor VII/*physiology
MH  - Humans
MH  - RNA, Messenger
MH  - Signal Transduction
MH  - *Thromboplastin
EDAT- 2017/12/22 06:00
MHDA- 2018/07/26 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/12/22 06:00 [entrez]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2018/07/26 06:00 [medline]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2017 Dec 18;49(6):931-936.