PMID- 29264808
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20180925
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1722
DP  - 2018
TI  - Methods to Study the Signal Transduction of the Surface Receptor Tyrosine Kinase 
      TrkB in Neurons.
PG  - 211-222
LID - 10.1007/978-1-4939-7553-2_14 [doi]
AB  - Receptor tyrosine kinases (RTK) belong to a major class of cell surface receptors 
      that transduce extracellular signals to elicit diverse intracellular responses. 
      Upon binding to specific ligand, the RTKs become dimerized and autophosphorylated 
      at tyrosine residues. This creates binding sites to recruit specific signaling 
      intermediates and hence trigger distinct signaling events. The cellular response 
      to a given RTK may be modified through the regulation of membrane insertion and 
      receptor internalization. Here we use Trk receptor and its ligand, the 
      neurotrophin brain-derived neurotrophic factor (BDNF), as an example to 
      illustrate the approaches (coimmunoprecipitation and biotinylation) to study the 
      surface expression and signal transduction mediated by this class of RTK in the 
      nervous system.
FAU - Lai, Kwok-On
AU  - Lai KO
AD  - School of Biomedical Sciences, LKS Faculty of Medicine, State Key Laboratory of 
      Brain and Cognitive Sciences, The University of Hong Kong, Hong Kong, SAR, China. 
      laiko@hku.hk.
FAU - Ip, Nancy Y
AU  - Ip NY
AD  - Division of Life Science, Molecular Neuroscience Center and State Key Laboratory 
      of Molecular Neuroscience, The Hong Kong University of Science and Technology, 
      Hong Kong, SAR, China. boip@ust.hk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ligands)
RN  - 42HK56048U (Tyrosine)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Biotinylation/*methods
MH  - Brain/cytology/embryology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Immunoprecipitation/*methods
MH  - Ligands
MH  - Mice
MH  - Neurons/*metabolism
MH  - Phosphorylation
MH  - Primary Cell Culture
MH  - Rats
MH  - Receptor, trkB/*metabolism
MH  - *Signal Transduction
MH  - Tyrosine/metabolism
OTO - NOTNLM
OT  - Kinase
OT  - Neuron
OT  - Phosphorylation
OT  - Signal transduction
OT  - Surface expression
EDAT- 2017/12/22 06:00
MHDA- 2018/08/15 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/12/22 06:00 [entrez]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
AID - 10.1007/978-1-4939-7553-2_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1722:211-222. doi: 10.1007/978-1-4939-7553-2_14.