PMID- 29265147
OWN - NLM
STAT- MEDLINE
DCOM- 20181228
LR  - 20210217
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Linking)
VI  - 10
IP  - 3
DP  - 2018 Jan 18
TI  - Technical challenges of working with extracellular vesicles.
PG  - 881-906
LID - 10.1039/c7nr08360b [doi]
AB  - Extracellular Vesicles (EVs) are gaining interest as central players in liquid 
      biopsies, with potential applications in diagnosis, prognosis and therapeutic 
      guidance in most pathological conditions. These nanosized particles transmit 
      signals determined by their protein, lipid, nucleic acid and sugar content, and 
      the unique molecular pattern of EVs dictates the type of signal to be transmitted 
      to recipient cells. However, their small sizes and the limited quantities that 
      can usually be obtained from patient-derived samples pose a number of challenges 
      to their isolation, study and characterization. These challenges and some 
      possible options to overcome them are discussed in this review.
FAU - Ramirez, Marcel I
AU  - Ramirez MI
AD  - Fundacao Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil and Universidade 
      Federal do Parana, Curitiba, PR, Brazil.
FAU - Amorim, Maria G
AU  - Amorim MG
AD  - CIPE, A.C.Camargo Cancer Center, Sao Paulo, SP, Brazil. 
      emmanuel@cipe.accamargo.org.br.
FAU - Gadelha, Catarina
AU  - Gadelha C
AD  - School of Life Sciences, University of Nottingham, Nottingham, UK.
FAU - Milic, Ivana
AU  - Milic I
AUID- ORCID: 0000-0001-7531-7561
AD  - School of Life and Health Sciences, Aston University, England, UK.
FAU - Welsh, Joshua A
AU  - Welsh JA
AUID- ORCID: 0000-0002-1097-9756
AD  - University of Southampton, Southampton, England, UK.
FAU - Freitas, Vanessa M
AU  - Freitas VM
AUID- ORCID: 0000-0001-9613-8626
AD  - Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Nawaz, Muhammad
AU  - Nawaz M
AUID- ORCID: 0000-0002-0792-8296
AD  - Universidade de Sao Paulo, Sao Paulo, SP, Brazil and University of Gothenburg, 
      Sweden.
FAU - Akbar, Naveed
AU  - Akbar N
AD  - Division of Cardiovascular Medicine, University of Oxford, Oxford, England, UK.
FAU - Couch, Yvonne
AU  - Couch Y
AUID- ORCID: 0000-0002-5623-5568
AD  - Acute Stroke Programme, RDM-Investigative Medicine, University of Oxford, Oxford, 
      England, UK.
FAU - Makin, Laura
AU  - Makin L
AD  - Sir William Dunn School of Pathology, University of Oxford, Oxford, England, UK.
FAU - Cooke, Fiona
AU  - Cooke F
AD  - University of St Andrews, St Andrews, Fife, Scotland, UK.
FAU - Vettore, Andre L
AU  - Vettore AL
AUID- ORCID: 0000-0001-8526-5478
AD  - Federal University of Sao Paulo campus Diadema, Diadema, Brazil.
FAU - Batista, Patricia X
AU  - Batista PX
AUID- ORCID: 0000-0002-6329-6449
AD  - Federal University of Sao Paulo campus Diadema, Diadema, Brazil.
FAU - Freezor, Roberta
AU  - Freezor R
AD  - London Metropolitan University, London, England, UK.
FAU - Pezuk, Julia A
AU  - Pezuk JA
AUID- ORCID: 0000-0001-5412-6619
AD  - Universidade Anhanguera de Sao Paulo, Sao Paulo, Brazil.
FAU - Rosa-Fernandes, Livia
AU  - Rosa-Fernandes L
AUID- ORCID: 0000-0003-1612-1950
AD  - Universidade de Sao Paulo, Sao Paulo, SP, Brazil and University of Southern 
      Denmark, Odense, Denmark.
FAU - Carreira, Ana Claudia O
AU  - Carreira ACO
AD  - Universidade de Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Devitt, Andrew
AU  - Devitt A
AUID- ORCID: 0000-0002-4651-6761
AD  - School of Life and Health Sciences, Aston University, England, UK.
FAU - Jacobs, Laura
AU  - Jacobs L
AD  - Technische Universitat Munchen, Munich, Germany.
FAU - Silva, Israel T
AU  - Silva IT
AUID- ORCID: 0000-0002-4687-1499
AD  - CIPE, A.C.Camargo Cancer Center, Sao Paulo, SP, Brazil. 
      emmanuel@cipe.accamargo.org.br.
FAU - Coakley, Gillian
AU  - Coakley G
AD  - Institute of Infection, Immunity and Inflammation, University of Glasgow, 
      Glasgow, Scotland, UK.
FAU - Nunes, Diana N
AU  - Nunes DN
AUID- ORCID: 0000-0003-0751-4701
AD  - CIPE, A.C.Camargo Cancer Center, Sao Paulo, SP, Brazil. 
      emmanuel@cipe.accamargo.org.br.
FAU - Carter, Dave
AU  - Carter D
AD  - Oxford Brookes University, Oxford, England, UK.
FAU - Palmisano, Giuseppe
AU  - Palmisano G
AUID- ORCID: 0000-0003-1336-6151
AD  - Universidade de Sao Paulo, Sao Paulo, SP, Brazil and IRCCS, Fondazione Santa 
      Lucia, Rome, Italy.
FAU - Dias-Neto, Emmanuel
AU  - Dias-Neto E
AUID- ORCID: 0000-0001-5670-8559
AD  - CIPE, A.C.Camargo Cancer Center, Sao Paulo, SP, Brazil. 
      emmanuel@cipe.accamargo.org.br and Universidade de Sao Paulo, Sao Paulo, SP, 
      Brazil.
LA  - eng
GR  - BB/E002080/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - MR/N01037X/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Review
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (Carbohydrates)
RN  - 0 (Lipids)
RN  - 0 (Nucleic Acids)
RN  - 0 (Proteins)
SB  - IM
MH  - Carbohydrates
MH  - Extracellular Vesicles/*chemistry
MH  - Humans
MH  - Lipids
MH  - Nucleic Acids
MH  - Prognosis
MH  - Proteins
EDAT- 2017/12/22 06:00
MHDA- 2018/12/29 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2018/12/29 06:00 [medline]
PHST- 2017/12/22 06:00 [entrez]
AID - 10.1039/c7nr08360b [doi]
PST - ppublish
SO  - Nanoscale. 2018 Jan 18;10(3):881-906. doi: 10.1039/c7nr08360b.