PMID- 29266822
OWN - NLM
STAT- MEDLINE
DCOM- 20191113
LR  - 20191113
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 12
IP  - 4
DP  - 2018 Apr
TI  - Segregation of motor and sensory axons regenerating through bicompartmental tubes 
      by combining extracellular matrix components with neurotrophic factors.
PG  - e1991-e2000
LID - 10.1002/term.2629 [doi]
AB  - Segregation of regenerating motor and sensory axons may be a good strategy to 
      improve selective functionality of regenerative interfaces to provide closed-loop 
      commands. Provided that extracellular matrix components and neurotrophic factors 
      exert guidance effects on different neuronal populations, we assessed in vivo the 
      potential of separating sensory and motor axons regenerating in a bicompartmental 
      Y-type tube, with each branch prefilled with an adequate combination of 
      extracellular matrix and neurotrophic factors. The severed rat sciatic nerve was 
      repaired using a bicompartmental tube filled with a collagen matrix enriched with 
      fibronectin (FN) and brain-derived neurotrophic factor (BDNF) encapsulated in 
      poly-lactic co-glycolic acid microspheres (FN + MP.BDNF) in one compartment to 
      preferentially attract motor axons and collagen enriched with laminin (LM) and 
      nerve growth factor (NGF) and neurotrophin-3 (NT-3) in microspheres 
      (LM + MP.NGF/NT-3) in the other compartment for promoting sensory axons 
      regeneration. Control animals were implanted with the same Y-tube with a collagen 
      matrix with microspheres (MP) containing PBS (Col + MP.PBS). By using retrotracer 
      labelling, we found that LM + MP.NGF/NT-3 did not attract higher number of 
      regenerated sensory axons compared with controls, and no differences were 
      observed in sensory functional recovery. However, FN + MP.BDNF guided a higher 
      number of regenerating motor axons compared with controls, improving also motor 
      recovery. A small proportion of sensory axons with large soma size, likely 
      proprioceptive neurons, was also attracted to the FN + MP.BDNF compartment. These 
      results demonstrate that muscular axonal guidance can be modulated in vivo by the 
      addition of fibronectin and BDNF.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Del Valle, Jaume
AU  - Del Valle J
AUID- ORCID: 0000-0002-6703-8244
AD  - Institute of Neurosciences and Department of Cell Biology, Physiology, and 
      Immunology, Universitat Autonoma de Barcelona, Bellaterra, Spain.
AD  - Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas 
      (CIBERNED), Bellaterra, Spain.
AD  - Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, and BIST, 
      Bellaterra, Spain.
FAU - Santos, Daniel
AU  - Santos D
AD  - Institute of Neurosciences and Department of Cell Biology, Physiology, and 
      Immunology, Universitat Autonoma de Barcelona, Bellaterra, Spain.
AD  - Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas 
      (CIBERNED), Bellaterra, Spain.
FAU - Delgado-Martinez, Ignacio
AU  - Delgado-Martinez I
AUID- ORCID: 0000-0003-1616-8943
AD  - Institute of Neurosciences and Department of Cell Biology, Physiology, and 
      Immunology, Universitat Autonoma de Barcelona, Bellaterra, Spain.
FAU - de la Oliva, Natalia
AU  - de la Oliva N
AUID- ORCID: 0000-0002-1263-2705
AD  - Institute of Neurosciences and Department of Cell Biology, Physiology, and 
      Immunology, Universitat Autonoma de Barcelona, Bellaterra, Spain.
FAU - Giudetti, Guido
AU  - Giudetti G
AUID- ORCID: 0000-0002-4543-5871
AD  - The Biorobotics Institute, Scuola Superiore Sant'Anna, Pontedera, Italy.
FAU - Micera, Silvestro
AU  - Micera S
AUID- ORCID: 0000-0003-4396-8217
AD  - The Biorobotics Institute, Scuola Superiore Sant'Anna, Pontedera, Italy.
AD  - Translational Neural Engineering Laboratory, Center for Neuroprosthetics and 
      Institute of Bioengineering, Ecole Polytechnique Federale de Lausanne (EPFL), 
      Lausanne, Switzerland.
FAU - Navarro, Xavier
AU  - Navarro X
AUID- ORCID: 0000-0001-9849-902X
AD  - Institute of Neurosciences and Department of Cell Biology, Physiology, and 
      Immunology, Universitat Autonoma de Barcelona, Bellaterra, Spain.
AD  - Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas 
      (CIBERNED), Bellaterra, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180128
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Axons/*metabolism/pathology
MH  - Extracellular Matrix/*chemistry
MH  - Female
MH  - Motor Neurons/*metabolism/pathology
MH  - *Nerve Growth Factors/chemistry/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regeneration/*drug effects
MH  - Sensory Receptor Cells/*metabolism/pathology
OTO - NOTNLM
OT  - Y-tube
OT  - axon regeneration
OT  - axonal guidance
OT  - extracellular matrix
OT  - motor axons
OT  - neurotrophic factors
OT  - sensory axons
EDAT- 2017/12/22 06:00
MHDA- 2019/11/14 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/07/25 00:00 [received]
PHST- 2017/10/30 00:00 [revised]
PHST- 2017/12/11 00:00 [accepted]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2019/11/14 06:00 [medline]
PHST- 2017/12/22 06:00 [entrez]
AID - 10.1002/term.2629 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2018 Apr;12(4):e1991-e2000. doi: 10.1002/term.2629. Epub 
      2018 Jan 28.