PMID- 29267216
OWN - NLM
STAT- MEDLINE
DCOM- 20180820
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 19
IP  - 1
DP  - 2017 Dec 21
TI  - Epigallocatechin-3-Gallate Suppresses Human Herpesvirus 8 Replication and Induces 
      ROS Leading to Apoptosis and Autophagy in Primary Effusion Lymphoma Cells.
LID - 10.3390/ijms19010016 [doi]
LID - 16
AB  - Epigallocatechin-3-gallate (EGCG), the major constituent of green tea, has been 
      shown to induce cell death in cancer cells. Primary effusion lymphoma (PEL) is an 
      aggressive neoplasm caused by human herpesvirus 8 (HHV8). In this study, we 
      examined the role of EGCG on PEL cells in cell death and HHV8 replication. We 
      performed trypan blue exclusion assay to assess the cell viability of PEL cells, 
      flow cytometry analysis to examine the cell cycle distribution and reactive 
      oxygen species (ROS) generation, caspase-3 activity to assay apoptosis, acridine 
      orange staining to determine autophagy, and immunoblotting to detect the protein 
      levels involved in apoptosis and autophagy as well as mitogen activated protein 
      kinases (MAPKs) activation upon EGCG treatment. The expression of the HHV8 lytic 
      gene was determined by luciferase reporter assay and reverse transcription-PCR, 
      and viral progeny production was determined by PCR. Results revealed that EGCG 
      induced cell death and ROS generation in PEL cells in a dose-dependent manner. 
      N-acetylcysteine (NAC) inhibited the EGCG-induced ROS and rescued the cell from 
      EGCG-induced cell death. Even though EGCG induced ROS generation in PEL cells, it 
      reduced the production of progeny virus from PEL cells without causing HHV8 
      reactivation. These results suggest that EGCG may represent a novel strategy for 
      the treatment of HHV8 infection and HHV8-associated lymphomas.
FAU - Tsai, Ching-Yi
AU  - Tsai CY
AD  - Department of Medical Laboratory Science and Biotechnology, Fooyin-University, 
      Kaohsiung 83102, Taiwan. a07646815@yahoo.com.tw.
FAU - Chen, Chang-Yu
AU  - Chen CY
AD  - Department of Medical Laboratory Science and Biotechnology, Fooyin-University, 
      Kaohsiung 83102, Taiwan. chencyallen1689@gmail.com.
FAU - Chiou, Yee-Hsuan
AU  - Chiou YH
AD  - Department of Pediatrics, Kaohsiung Veterans General Hospital, Kaohsiung 83102, 
      Taiwan. chysn@ms6.hinet.net.
FAU - Shyu, Huey-Wen
AU  - Shyu HW
AD  - Department of Medical Laboratory Science and Biotechnology, Fooyin-University, 
      Kaohsiung 83102, Taiwan. shyuhw@hotmail.com.
FAU - Lin, Kuan-Hua
AU  - Lin KH
AD  - Department of Medical Laboratory Science and Biotechnology, Fooyin-University, 
      Kaohsiung 83102, Taiwan. su6473@mail2000.com.tw.
FAU - Chou, Miao-Chen
AU  - Chou MC
AD  - Department of Medical Laboratory Science and Biotechnology, Fooyin-University, 
      Kaohsiung 83102, Taiwan. miaochen119@gmail.com.
FAU - Huang, Mei-Han
AU  - Huang MH
AD  - Department of Medical Laboratory Science and Biotechnology, Fooyin-University, 
      Kaohsiung 83102, Taiwan. hmhmhtw8@gmail.com.
FAU - Wang, Yi-Fen
AU  - Wang YF
AD  - Department of Medical Laboratory Science and Biotechnology, Fooyin-University, 
      Kaohsiung 83102, Taiwan. sc071@fy.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20171221
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Antiviral Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Antiviral Agents/pharmacology
MH  - Apoptosis/*drug effects
MH  - Autophagy/*drug effects
MH  - Catechin/*analogs & derivatives/pharmacology/therapeutic use
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - HEK293 Cells
MH  - Herpesviridae Infections/complications/*drug therapy
MH  - Herpesvirus 8, Human/*drug effects
MH  - Humans
MH  - Lymphoma, Primary Effusion/*drug therapy/virology
MH  - Reactive Oxygen Species/*metabolism
MH  - Virus Replication/*drug effects
PMC - PMC5795967
OTO - NOTNLM
OT  - EGCG
OT  - ROS
OT  - apoptosis
OT  - autophagy
OT  - primary effusion lymphoma
COIS- The authors declare no conflict of interest.
EDAT- 2017/12/22 06:00
MHDA- 2018/08/21 06:00
PMCR- 2018/01/01
CRDT- 2017/12/22 06:00
PHST- 2017/11/26 00:00 [received]
PHST- 2017/12/15 00:00 [revised]
PHST- 2017/12/18 00:00 [accepted]
PHST- 2017/12/22 06:00 [entrez]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2018/08/21 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - ijms19010016 [pii]
AID - ijms-19-00016 [pii]
AID - 10.3390/ijms19010016 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Dec 21;19(1):16. doi: 10.3390/ijms19010016.