PMID- 29268120
OWN - NLM
STAT- MEDLINE
DCOM- 20180516
LR  - 20181202
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 150
DP  - 2018 Apr 15
TI  - Experimental exposure of blue mussels (Mytilus galloprovincialis) to high levels 
      of benzo[a]pyrene and possible implications for human health.
PG  - 96-103
LID - S0147-6513(17)30873-4 [pii]
LID - 10.1016/j.ecoenv.2017.12.038 [doi]
AB  - Polycyclic aromatic hydrocarbons (PAHs) are lipophilic compounds able to 
      accumulate in the food chain. Mussels showed to bioaccumulate contaminants, such 
      as PAHs, so that recurrent consumption of such contaminated food represents a 
      risk for human health. This study was aimed to elucidate if acute exposure of 
      Mediterranean blue mussel (Mytilus galloprovincialis), a bivalve of great 
      economic importance in several countries, to a PAH, benzo[a]pyrene (B[a]P), at 
      doses able to induce cytochrome P450 1A (CYP1A) and pathological changes in 
      mussel gills, can produce accumulation in soft tissue. We explored the cytotoxic 
      effects (cell viability, DNA laddering, and glutathione levels) of in vitro 
      exposure of human peripheral blood mononuclear cells (PBMCs) to organic extracts 
      obtained from blue mussels previously exposed for 12 and 72h via water to B[a]P 
      (0.5-1mg/L). In our experimental conditions, B[a]P induced CYP1A induction and 
      morphological changes in mussel gills and a significant B[a]P accumulation in 
      soft tissue. Conversely, exposing PBMCs to organic extracts obtained from 
      contaminated mussels, resulted in a significant reduction of cell viability and 
      cell glutathione content, and in an increase in DNA laddering. This confirms that 
      consumption of mussels from B[a]P polluted waters might affect human health. Our 
      data lead us to suggest that CYP1A activity in mussel gills may be useful (more 
      than the amount of detected PAHs in the mussel edible tissue) as a marker in 
      assessment of risk for health of consumers exposed to PAHs through ingestion of 
      shellfish.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Speciale, A
AU  - Speciale A
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
FAU - Zena, R
AU  - Zena R
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
FAU - Calabro, C
AU  - Calabro C
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
FAU - Bertuccio, C
AU  - Bertuccio C
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
FAU - Aragona, M
AU  - Aragona M
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
FAU - Saija, A
AU  - Saija A
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
FAU - Trombetta, D
AU  - Trombetta D
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
FAU - Cimino, F
AU  - Cimino F
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy. Electronic address: 
      fcimino@unime.it.
FAU - Lo Cascio, P
AU  - Lo Cascio P
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, 98168 Messina, Italy.
LA  - eng
PT  - Journal Article
DEP - 20171219
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Water Pollutants, Chemical)
RN  - 3417WMA06D (Benzo(a)pyrene)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/analysis/*toxicity
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Gills/chemistry/drug effects/pathology
MH  - Humans
MH  - Leukocytes, Mononuclear/drug effects
MH  - Mytilus edulis/*drug effects
MH  - Risk Assessment
MH  - Water Pollutants, Chemical/analysis/*toxicity
OTO - NOTNLM
OT  - Benzo[a]pyrene
OT  - CYP1A
OT  - Human health
OT  - Mytilus galloprovincialis
OT  - PBMCs
EDAT- 2017/12/22 06:00
MHDA- 2018/05/17 06:00
CRDT- 2017/12/22 06:00
PHST- 2017/08/11 00:00 [received]
PHST- 2017/12/13 00:00 [revised]
PHST- 2017/12/14 00:00 [accepted]
PHST- 2017/12/22 06:00 [pubmed]
PHST- 2018/05/17 06:00 [medline]
PHST- 2017/12/22 06:00 [entrez]
AID - S0147-6513(17)30873-4 [pii]
AID - 10.1016/j.ecoenv.2017.12.038 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2018 Apr 15;150:96-103. doi: 
      10.1016/j.ecoenv.2017.12.038. Epub 2017 Dec 19.