PMID- 29270753
OWN - NLM
STAT- MEDLINE
DCOM- 20180726
LR  - 20181113
IS  - 1993-1352 (Electronic)
IS  - 1672-0733 (Linking)
VI  - 37
IP  - 6
DP  - 2017 Dec
TI  - Acetyl-L-carnitine: An effective antioxidant against cryo-damage on human 
      spermatozoa with asthenospermia.
PG  - 915-921
LID - 10.1007/s11596-017-1827-4 [doi]
AB  - A variety of natural and artificial cryoprotectant extenders have been explored 
      to enhance sperm recovery following cryopreservation-thawing process. The current 
      investigation is aimed at evaluating the effect of acetyl-L-carnitine on human 
      spermatozoa and reactive species oxygen (ROS) level after freezing-thawing 
      process. The spermatozoa were collected from 35 male patients diagnosed as having 
      asthenospermia. The cryopreservation of human spermatozoa treated with 
      acetyl-L-carnitine at different concentrations (group B: 2.5 mmol/L, group C: 7.5 
      mmol/L, group D: 15 mmol/L) was compared with control (group A: no 
      acetyl-L-carnitine given). For the frozen-thawed spermatozoa, the viability, 
      motility and DNA integrity were measured by comet assay, acrosome integrity by 
      FITC-PNA staining and ROS level was determined in each group. The results showed 
      that there were no significant differences in motility and viability between 
      group A and group B, while the motility and viability of spermatozoa in group C 
      and group D were significantly increased as compared with those in group A. As 
      compared with group A, the values for DNA integrity parameters including comet 
      rate (CR), tail DNA percentage (TD), tail length (TL) and Oliver tail moment 
      (OTM) were significantly reduced in group C and group D. Group C and group D also 
      displayed a higher proportion of intact acrosome than group A. No significant 
      difference in ROS level was found between group A and group B, while with the 
      increase in acetyl-L-carnitine concentration, the ROS level in groups C and D was 
      significantly reduced as compared with that in group A. In conclusion, 
      acetyl-L-carnitine at a concentration of 7.5 mmol/L is an effective antioxidant 
      against cryo-damage on post-thawed human spermatozoa.
FAU - Zou, Yu-Jie
AU  - Zou YJ
AD  - Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
FAU - Yang, Jing
AU  - Yang J
AD  - Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China. dryangqing@hotmail.com.
FAU - Chang, Shuo
AU  - Chang S
AD  - Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
FAU - Xu, Wang-Ming
AU  - Xu WM
AD  - Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
FAU - Yin, Tai-Lang
AU  - Yin TL
AD  - Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
FAU - Long, Wen
AU  - Long W
AD  - Reproductive Medicine Center, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20171221
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Antioxidants)
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 6DH1W9VH8Q (Acetylcarnitine)
SB  - IM
MH  - Acetylcarnitine/*pharmacology
MH  - Acrosome/*drug effects/metabolism/ultrastructure
MH  - Antioxidants/*pharmacology
MH  - Asthenozoospermia/metabolism/*physiopathology
MH  - Case-Control Studies
MH  - Cell Survival/drug effects
MH  - Cryopreservation/*methods
MH  - Cryoprotective Agents/*pharmacology
MH  - Humans
MH  - Infertility, Male/metabolism/physiopathology
MH  - Male
MH  - Oxidative Stress
MH  - Reactive Oxygen Species/antagonists & inhibitors/metabolism
MH  - Sperm Motility/drug effects
OTO - NOTNLM
OT  - DNA damage
OT  - acetyl-L-carnitine
OT  - acrosome integrity
OT  - human spermatozoa
EDAT- 2017/12/23 06:00
MHDA- 2018/07/27 06:00
CRDT- 2017/12/23 06:00
PHST- 2017/06/10 00:00 [received]
PHST- 2017/10/16 00:00 [revised]
PHST- 2017/12/23 06:00 [entrez]
PHST- 2017/12/23 06:00 [pubmed]
PHST- 2018/07/27 06:00 [medline]
AID - 10.1007/s11596-017-1827-4 [pii]
AID - 10.1007/s11596-017-1827-4 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2017 Dec;37(6):915-921. doi: 
      10.1007/s11596-017-1827-4. Epub 2017 Dec 21.