PMID- 29270923
OWN - NLM
STAT- MEDLINE
DCOM- 20190815
LR  - 20190815
IS  - 1439-3646 (Electronic)
IS  - 0947-7349 (Linking)
VI  - 127
IP  - 4
DP  - 2019 Apr
TI  - Expression and Significance of MMPs in Synovial Fluid, Serum and PBMC Culture 
      Supernatant Stimulated by LPS in Osteoarthritis Patients With or Without 
      Diabetes.
PG  - 195-202
LID - 10.1055/s-0043-122223 [doi]
AB  - OBJECTIVE: Patients with Type 2 Diabetes mellitus (T2DM) are prone to 
      osteoarthritis (OA). Matrix metalloproteinases (MMPs), an essential modulator in 
      cartilage matrix homeostasis, increase in T2DM and OA. We aimed to ascertain the 
      expression difference of MMPs and function in mononuclear cells after stimulating 
      by lipopolysaccharide (LPS) in OA patients with or without diabetes. METHODS: 30 
      knee OA patients without T2DM (OA group), 20 knee OA patients with T2DM (DM-OA 
      group) and 5 healthy volunteers recruited as control were enrolled from January 
      2016 to January 2017. The expression levels of MMPs in both serum and synovial 
      fluid were initially detected in three groups by enzyme-linked immunosorbent 
      assay (ELISA). After stimulation of peripheral blood mononuclear cell (PBMC) with 
      LPS, the release of MMPs were determined and evaluated. RESULTS: The expression 
      of MMP-1, -7, -8, -9, -10 and -12 in synovial fluid in DM-OA group were 
      significantly higher than in OA group and healthy control. The expression of 
      MMP-1 and -7 in serum were highest in DM-OA group. LPS significantly promotes the 
      production of MMP-1, -8, -9 and -10 in PBMC of each group after 4 h stimulation. 
      It is worth to note that the LPS-stimulated MMP-8 and -9 elevations were more 
      prominent in DM-OA group compared with their counterparts. CONCLUSION: High 
      levels of MMP-1, -7, -8, -9, -10, and -12 in the synovial fluid might be one of 
      important reasons that diabetes patients are more frequently suffered from OA. 
      Inflammation-induced malfunction of mononuclear cells would stimulate MMP-8 and 
      -9 secretion to various extents.
CI  - (c) Georg Thieme Verlag KG Stuttgart . New York.
FAU - Luo, Simin
AU  - Luo S
AD  - The First Affiliated Hospital, Jinan University, Guangzhou, China.
AD  - Institute of Orthopaedic Disease Research, Jinan University, Guangzhou, China.
FAU - Shi, Qiping
AU  - Shi Q
AD  - The First Affiliated Hospital, Jinan University, Guangzhou, China.
FAU - Chen, Junyuan
AU  - Chen J
AD  - The First Affiliated Hospital, Jinan University, Guangzhou, China.
AD  - Institute of Orthopaedic Disease Research, Jinan University, Guangzhou, China.
FAU - Wang, Huajun
AU  - Wang H
AD  - The First Affiliated Hospital, Jinan University, Guangzhou, China.
AD  - Institute of Orthopaedic Disease Research, Jinan University, Guangzhou, China.
FAU - Wu, Wenrui
AU  - Wu W
AD  - The First Affiliated Hospital, Jinan University, Guangzhou, China.
AD  - Institute of Orthopaedic Disease Research, Jinan University, Guangzhou, China.
FAU - Zha, Zhengang
AU  - Zha Z
AD  - The First Affiliated Hospital, Jinan University, Guangzhou, China.
AD  - Institute of Orthopaedic Disease Research, Jinan University, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20171221
PL  - Germany
TA  - Exp Clin Endocrinol Diabetes
JT  - Experimental and clinical endocrinology & diabetes : official journal, German 
      Society of Endocrinology [and] German Diabetes Association
JID - 9505926
RN  - 0 (Lipopolysaccharides)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
MH  - Adult
MH  - Aged
MH  - Cells, Cultured
MH  - Comorbidity
MH  - Diabetes Mellitus, Type 2/epidemiology/immunology/*metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/immunology/*metabolism
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Lipopolysaccharides
MH  - Male
MH  - Matrix Metalloproteinases/blood/*metabolism
MH  - Middle Aged
MH  - Osteoarthritis, Knee/epidemiology/immunology/*metabolism
MH  - Synovial Fluid/*metabolism
COIS- No conflict of interest has been declared by the authors.
EDAT- 2017/12/23 06:00
MHDA- 2019/08/16 06:00
CRDT- 2017/12/23 06:00
PHST- 2017/12/23 06:00 [pubmed]
PHST- 2019/08/16 06:00 [medline]
PHST- 2017/12/23 06:00 [entrez]
AID - 10.1055/s-0043-122223 [doi]
PST - ppublish
SO  - Exp Clin Endocrinol Diabetes. 2019 Apr;127(4):195-202. doi: 
      10.1055/s-0043-122223. Epub 2017 Dec 21.