PMID- 29272757
OWN - NLM
STAT- MEDLINE
DCOM- 20180302
LR  - 20180302
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1073
DP  - 2018 Jan 15
TI  - Putative identification of components in Zengye Decoction and their effects on 
      glucose consumption and lipogenesis in insulin-induced insulin-resistant HepG2 
      cells.
PG  - 145-153
LID - S1570-0232(17)31152-2 [pii]
LID - 10.1016/j.jchromb.2017.12.019 [doi]
AB  - Zengye Decoction (ZYD) is a well-known traditional medicine in China used for 
      treating diseases associated with "Yin deficiency" such as diabetes. However, 
      little information is available on its components, pharmacological effects and 
      underlying mechanisms. This study was designed to identify its active components 
      and evaluate the effects and mechanisms of ZYD on glucose consumption and 
      lipogenesis in insulin-induced insulin-resistant (IR)-HepG2 cells. In this study, 
      45 compounds of ZYD were putatively identified, in which the iridoid glycosides 
      such as catalpol, aucubin and harpagide were identified as the main components. 
      The insulin-resistant (IR)-HepG2 cell model was established and the effect of ZYD 
      at three doses (0.17, 0.5 and 1.5 mug/mL) on cell growth was evaluated with an 
      IncuCyte live-cell imaging system. The effects of ZYD on glucose consumption and 
      uptake were evaluated by glucose consumption and uptake assay. Meanwhile, the 
      effect of ZYD on lipogenesis was investigated in IR-HepG2 cells by oil red O 
      (ORO) staining. Western blot was applied to observe the changes in some of the 
      key factors involved in glucose metabolism and lipogenesis. It was found that the 
      ZYD at a dose of 1.5 mug/mL exhibited an inhibitory activity on IR-HepG2 cell 
      growth. Besides, ZYD at doses of 0.5 and 1.5 mug/mL accelerated the glucose 
      consumption, glucose uptake and reduced the lipogenesis in the IR-HepG2 cells. 
      Western blot studies revealed that ZYD phosphorylated AMP-activated protein 
      kinase alpha subunits (AMPKalpha), upregulated hexokinase (HK), phosphorylated acetyl-CoA 
      carboxylase alpha (pACC1) and carnitine palmitoyltransferase 1A (CPT1A) in the 
      IR-HepG2 cells. These results indicate ZYD promotes glucose consumption and 
      uptake, and attenuates lipogenesis in IR-HepG2 cells, which may be involved in 
      activating AMPK and regulating its downstream factors including HK, pACC1 and 
      CPT1A.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Liu, Zhenzhen
AU  - Liu Z
AD  - School of Medicine, Yangtze University, Jingzhou 434000, China.
FAU - Kuang, Wenhua
AU  - Kuang W
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of 
      Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, 
      China; School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, 
      China.
FAU - Xu, Xi
AU  - Xu X
AD  - School of Medicine, Yangtze University, Jingzhou 434000, China.
FAU - Li, Dandan
AU  - Li D
AD  - School of Medicine, Yangtze University, Jingzhou 434000, China.
FAU - Zhu, Wufu
AU  - Zhu W
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of 
      Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, 
      China.
FAU - Lan, Zhou
AU  - Lan Z
AD  - Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, School of 
      Pharmacy, Jiangxi Science and Technology Normal University, Nanchang 330013, 
      China. Electronic address: A83875785@126.com.
FAU - Zhang, Xu
AU  - Zhang X
AD  - School of Life Sciences, Tsinghua University, Beijing 100084, China.
LA  - eng
PT  - Journal Article
DEP - 20171212
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Insulin)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Glucose/*metabolism
MH  - Glycolysis/drug effects
MH  - Hep G2 Cells
MH  - Humans
MH  - Insulin/*metabolism
MH  - *Insulin Resistance
MH  - Lipogenesis/*drug effects
OTO - NOTNLM
OT  - AMPKalpha
OT  - Glucose consumption
OT  - Glucose uptake
OT  - Hexokinase
OT  - Insulin resistant
OT  - Zengye Decoction
EDAT- 2017/12/23 06:00
MHDA- 2018/03/03 06:00
CRDT- 2017/12/23 06:00
PHST- 2017/07/06 00:00 [received]
PHST- 2017/11/29 00:00 [revised]
PHST- 2017/12/10 00:00 [accepted]
PHST- 2017/12/23 06:00 [pubmed]
PHST- 2018/03/03 06:00 [medline]
PHST- 2017/12/23 06:00 [entrez]
AID - S1570-0232(17)31152-2 [pii]
AID - 10.1016/j.jchromb.2017.12.019 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 15;1073:145-153. doi: 
      10.1016/j.jchromb.2017.12.019. Epub 2017 Dec 12.