PMID- 29273711
OWN - NLM
STAT- MEDLINE
DCOM- 20190726
LR  - 20190726
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
IP  - 1
DP  - 2017 Dec 22
TI  - LUCS (Light-Up Cell System), a universal high throughput assay for homeostasis 
      evaluation in live cells.
PG  - 18069
LID - 10.1038/s41598-017-18211-2 [doi]
LID - 18069
AB  - Observations of fluorescent cyanine dye behavior under illumination at 500 nm 
      lead to a novel concept in cell biology allowing the development of a new live 
      cell assay called LUCS, for Light-Up Cell System, measuring homeostasis in live 
      cells. Optimization of the LUCS process resulted in a standardized, 
      straightforward and high throughput assay with applications in toxicity 
      assessment. The mechanisms of the LUCS process were investigated. Electron 
      Paramagnetic Resonance experiments showed that the singlet oxygen and hydroxyl 
      radical are involved downstream of the light effect, presumably leading to 
      deleterious oxidative stress that massively opens access of the dye to its 
      intracellular target. Reversible modulation of LUCS by both verapamil and proton 
      availability indicated that plasma membrane proton/cation antiporters, possibly 
      of the MATE drug efflux transport family, are involved. A mechanistic model is 
      presented. Our data show that intracellular oxidation can be controlled by tuning 
      light energy, opening applications in regulatory purposes, anti-oxidant research, 
      chemotherapy efficacy and dynamic phototherapy strategies.
FAU - Derick, Sylvain
AU  - Derick S
AD  - Anti Oxidant Power AOP/MH2F-LAAS/CNRS, 7 avenue du Colonel Roche, BP 54200, 
      Toulouse Cedex, 31031, France.
FAU - Gironde, Camille
AU  - Gironde C
AD  - Anti Oxidant Power AOP/MH2F-LAAS/CNRS, 7 avenue du Colonel Roche, BP 54200, 
      Toulouse Cedex, 31031, France.
FAU - Perio, Pierre
AU  - Perio P
AD  - Universite de Toulouse, PHARMA-DEV, UPS-IRD, 118, route de Narbonne, Toulouse 
      Cedex, 31062, France.
FAU - Reybier, Karine
AU  - Reybier K
AD  - Universite de Toulouse, PHARMA-DEV, UPS-IRD, 118, route de Narbonne, Toulouse 
      Cedex, 31062, France.
FAU - Nepveu, Francoise
AU  - Nepveu F
AD  - Universite de Toulouse, PHARMA-DEV, UPS-IRD, 118, route de Narbonne, Toulouse 
      Cedex, 31062, France.
FAU - Jauneau, Alain
AU  - Jauneau A
AD  - CNRS, Institut Federatif de Recherche 3450, Plateforme Imagerie, Pole de 
      Biotechnologie Vegetale, Castanet-Tolosan, 31326, France.
FAU - Furger, Christophe
AU  - Furger C
AD  - Anti Oxidant Power AOP/MH2F-LAAS/CNRS, 7 avenue du Colonel Roche, BP 54200, 
      Toulouse Cedex, 31031, France. cfurger@laas.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171222
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Fluorescent Dyes)
RN  - 17778-80-2 (Singlet Oxygen)
RN  - 3352-57-6 (Hydroxyl Radical)
MH  - *Electron Spin Resonance Spectroscopy
MH  - *Fluorescent Dyes
MH  - *Homeostasis
MH  - Hydroxyl Radical
MH  - Singlet Oxygen
PMC - PMC5741755
COIS- Dr Sylvain Derick and Dr Christophe Furger declare competing financial interest 
      due to patent ownership on LUCS method. Other authors declare no competing 
      financial interest.
EDAT- 2017/12/24 06:00
MHDA- 2019/07/28 06:00
PMCR- 2017/12/22
CRDT- 2017/12/24 06:00
PHST- 2017/06/23 00:00 [received]
PHST- 2017/12/06 00:00 [accepted]
PHST- 2017/12/24 06:00 [entrez]
PHST- 2017/12/24 06:00 [pubmed]
PHST- 2019/07/28 06:00 [medline]
PHST- 2017/12/22 00:00 [pmc-release]
AID - 10.1038/s41598-017-18211-2 [pii]
AID - 18211 [pii]
AID - 10.1038/s41598-017-18211-2 [doi]
PST - epublish
SO  - Sci Rep. 2017 Dec 22;7(1):18069. doi: 10.1038/s41598-017-18211-2.