PMID- 29274031
OWN - NLM
STAT- MEDLINE
DCOM- 20191002
LR  - 20191002
IS  - 1557-1904 (Electronic)
IS  - 1557-1890 (Print)
IS  - 1557-1890 (Linking)
VI  - 13
IP  - 2
DP  - 2018 Jun
TI  - Sex Differences in Early Postnatal Microglial Colonization of the Developing Rat 
      Hippocampus Following a Single-Day Alcohol Exposure.
PG  - 189-203
LID - 10.1007/s11481-017-9774-1 [doi]
AB  - Microglia are involved in various homeostatic processes in the brain, including 
      phagocytosis, apoptosis, and synaptic pruning. Sex differences in microglia 
      colonization of the developing brain have been reported, but have not been 
      established following alcohol insult. Developmental alcohol exposure represents a 
      neuroimmune challenge that may contribute to cognitive dysfunction prevalent in 
      humans with Fetal Alcohol Spectrum Disorders (FASD) and in rodent models of FASD. 
      Most studies have investigated neuroimmune activation following adult alcohol 
      exposure or following multiple exposures. The current study uses a single day 
      binge alcohol exposure model (postnatal day [PD] 4) to examine sex differences in 
      the neuroimmune response in the developing rat hippocampus on PD5 and 8. The 
      neuroimmune response was evaluated through measurement of microglial number and 
      cytokine gene expression at both time points. Male pups had higher microglial 
      number compared to females in many hippocampal subregions on PD5, but this 
      difference disappeared by PD8, unless exposed to alcohol. Expression of 
      pro-inflammatory marker CD11b was higher on PD5 in alcohol-exposed (AE) females 
      compared to AE males. After alcohol exposure, C-C motif chemokine ligand 4 (CCL4) 
      was significantly increased in female AE pups on PD5 and PD8. Tumor necrosis 
      factor-alpha (TNF-alpha) levels were also upregulated by AE in males on PD8. The results 
      demonstrate a clear difference between the male and female neuroimmune response 
      to an AE challenge, which also occurs in a time-dependent manner. These findings 
      are significant as they add to our knowledge of specific sex-dependent effects of 
      alcohol exposure on microglia within the developing brain.
FAU - Ruggiero, M J
AU  - Ruggiero MJ
AD  - Department of Psychological and Brain Sciences, University of Delaware, 108 Wolf 
      Hall, Newark, DE, 19716, USA.
FAU - Boschen, K E
AU  - Boschen KE
AD  - Department of Psychological and Brain Sciences, University of Delaware, 108 Wolf 
      Hall, Newark, DE, 19716, USA.
FAU - Roth, T L
AU  - Roth TL
AD  - Department of Psychological and Brain Sciences, University of Delaware, 108 Wolf 
      Hall, Newark, DE, 19716, USA.
FAU - Klintsova, A Y
AU  - Klintsova AY
AD  - Department of Psychological and Brain Sciences, University of Delaware, 108 Wolf 
      Hall, Newark, DE, 19716, USA. klintsova@psych.udel.edu.
LA  - eng
GR  - P20 GM103653/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20171222
PL  - United States
TA  - J Neuroimmune Pharmacol
JT  - Journal of neuroimmune pharmacology : the official journal of the Society on 
      NeuroImmune Pharmacology
JID - 101256586
RN  - 0 (Central Nervous System Depressants)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Central Nervous System Depressants/toxicity
MH  - Ethanol/*toxicity
MH  - Female
MH  - Fetal Alcohol Spectrum Disorders/pathology
MH  - Hippocampus/*drug effects
MH  - Male
MH  - Microglia/*drug effects
MH  - Neurogenesis/*drug effects
MH  - Neuroimmunomodulation/drug effects
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/*pathology
MH  - Rats
MH  - Rats, Long-Evans
MH  - Sex Characteristics
PMC - PMC5997457
MID - NIHMS971745
OTO - NOTNLM
OT  - FASD
OT  - Immune
OT  - Microglia
OT  - Postnatal brain
OT  - Sex differences
EDAT- 2017/12/24 06:00
MHDA- 2019/10/03 06:00
PMCR- 2018/06/12
CRDT- 2017/12/24 06:00
PHST- 2017/08/29 00:00 [received]
PHST- 2017/12/03 00:00 [accepted]
PHST- 2017/12/24 06:00 [pubmed]
PHST- 2019/10/03 06:00 [medline]
PHST- 2017/12/24 06:00 [entrez]
PHST- 2018/06/12 00:00 [pmc-release]
AID - 10.1007/s11481-017-9774-1 [pii]
AID - 10.1007/s11481-017-9774-1 [doi]
PST - ppublish
SO  - J Neuroimmune Pharmacol. 2018 Jun;13(2):189-203. doi: 10.1007/s11481-017-9774-1. 
      Epub 2017 Dec 22.