PMID- 29274624
OWN - NLM
STAT- MEDLINE
DCOM- 20180831
LR  - 20180831
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 55
DP  - 2018 Feb
TI  - Interferon regulatory factor 5 (IRF5) regulates the expression of matrix 
      metalloproteinase-3 (MMP-3) in human chondrocytes.
PG  - 231-236
LID - S1567-5769(17)30457-5 [pii]
LID - 10.1016/j.intimp.2017.11.035 [doi]
AB  - Matrix metalloproteinase-3 (MMP-3) plays a pivotal role in the destruction of 
      articular cartilage in osteoarthritis (OA). The regulation of gene expression of 
      MMP-3 is complicated. Interferon regulatory factor 5 (IRF5) is a member of the 
      interferon regulatory factor family of transcription factors. Little information 
      regarding the biological function of IRF5 on chondrocytes and the pathogenesis of 
      OA has been reported. In the current study, for the first time, we report that 
      IRF5 is expressed in human primary chondrocytes and human chondrosarcoma cell 
      line SW1353 cells. In addition, IRF5 is upregulated in response to TNF-alpha 
      treatment in a dose dependent manner. Interestingly, IRF5 is significantly higher 
      in chondrocytes from OA patients compared to those from normal subjects. Notably, 
      IRF5 mediates TNF-alpha- induced expression of MMP-3 in chondrocytes. Overexpression 
      of IRF5 promotes the expression of MMP-3, however, knockdown of IRF5 reduces the 
      expression of MMP-3. Mechanistically, IRF5 is able to enhance the transcription 
      of MMP-3 by binding to its promoter. Also, we found that NF-kappaB was involved in 
      the effects of IRF-5 on MMP-3 expression. These findings suggest that IRF5 might 
      be a novel pharmacological target for the treatment of OA and RA.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Guo, Lin
AU  - Guo L
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China.
FAU - Hao, Ruihu
AU  - Hao R
AD  - Department of Orthopedics, Affiliated Zhongshan hospital of Dalian University, 
      Xi'an, China.
FAU - Tian, Fengde
AU  - Tian F
AD  - Department of Orthopedics, Affiliated Zhongshan hospital of Dalian University, 
      Xi'an, China.
FAU - An, Ning
AU  - An N
AD  - Department of Orthopedics, Affiliated Zhongshan hospital of Dalian University, 
      Xi'an, China.
FAU - Wang, Kunzheng
AU  - Wang K
AD  - Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, China. Electronic address: wangkz068@126.com.
LA  - eng
PT  - Journal Article
DEP - 20171222
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (IRF5 protein, human)
RN  - 0 (Interferon Regulatory Factors)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Cartilage, Articular/*pathology
MH  - Chondrosarcoma/*genetics/immunology
MH  - Gene Expression Regulation
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Interferon Regulatory Factors/*genetics/metabolism
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Molecular Targeted Therapy
MH  - NF-kappa B/metabolism
MH  - Osteoarthritis/*genetics/immunology
MH  - RNA, Small Interfering/genetics
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Chondrocytes
OT  - Interferon regulatory factor 5 (IRF5)
OT  - Matrix metalloproteinase-3
OT  - Osteoarthritis
EDAT- 2017/12/24 06:00
MHDA- 2018/09/01 06:00
CRDT- 2017/12/24 06:00
PHST- 2017/09/05 00:00 [received]
PHST- 2017/11/23 00:00 [revised]
PHST- 2017/11/27 00:00 [accepted]
PHST- 2017/12/24 06:00 [pubmed]
PHST- 2018/09/01 06:00 [medline]
PHST- 2017/12/24 06:00 [entrez]
AID - S1567-5769(17)30457-5 [pii]
AID - 10.1016/j.intimp.2017.11.035 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2018 Feb;55:231-236. doi: 10.1016/j.intimp.2017.11.035. Epub 
      2017 Dec 22.