PMID- 29276152
OWN - NLM
STAT- MEDLINE
DCOM- 20181226
LR  - 20210109
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 10
IP  - 1
DP  - 2018 Jan 9
TI  - In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells 
      Corresponds to In Vivo Waves of Hematopoietic Cell Generation.
PG  - 151-165
LID - S2213-6711(17)30526-X [pii]
LID - 10.1016/j.stemcr.2017.11.018 [doi]
AB  - In vivo hematopoietic generation occurs in waves of primitive and definitive cell 
      emergence. Differentiation cultures of pluripotent embryonic stem cells (ESCs) 
      offer an accessible source of hematopoietic cells for blood-related research and 
      therapeutic strategies. However, despite many approaches, it remains a goal to 
      robustly generate hematopoietic progenitor and stem cells (HP/SCs) in vitro from 
      ESCs. This is partly due to the inability to efficiently promote, enrich, and/or 
      molecularly direct hematopoietic emergence. Here, we use Gata2Venus (G2V) and 
      Ly6a(SCA1)GFP (LG) reporter ESCs, derived from well-characterized mouse models of 
      HP/SC emergence, to show that during in vitro differentiation they report 
      emergent waves of primitive hematopoietic progenitor cells (HPCs), definitive 
      HPCs, and B-lymphoid cell potential. These results, facilitated by enrichment of 
      single and double reporter cells with HPC properties, demonstrate that in vitro 
      ESC differentiation approximates the waves of hematopoietic cell generation found 
      in vivo, thus raising possibilities for enrichment of rare ESC-derived HP/SCs.
CI  - Copyright (c) 2017 The Author(s). Published by Elsevier Inc. All rights reserved.
FAU - Kauts, Mari-Liis
AU  - Kauts ML
AD  - Erasmus Medical Center Stem Cell Institute, Department of Cell Biology, Erasmus 
      Medical Center, Rotterdam, the Netherlands; Centre for Inflammation Research, 
      Queens Medical Research Institute, University of Edinburgh, Edinburgh, UK.
FAU - Rodriguez-Seoane, Carmen
AU  - Rodriguez-Seoane C
AD  - Centre for Inflammation Research, Queens Medical Research Institute, University 
      of Edinburgh, Edinburgh, UK.
FAU - Kaimakis, Polynikis
AU  - Kaimakis P
AD  - Erasmus Medical Center Stem Cell Institute, Department of Cell Biology, Erasmus 
      Medical Center, Rotterdam, the Netherlands.
FAU - Mendes, Sandra C
AU  - Mendes SC
AD  - Erasmus Medical Center Stem Cell Institute, Department of Cell Biology, Erasmus 
      Medical Center, Rotterdam, the Netherlands.
FAU - Cortes-Lavaud, Xabier
AU  - Cortes-Lavaud X
AD  - Centre for Inflammation Research, Queens Medical Research Institute, University 
      of Edinburgh, Edinburgh, UK.
FAU - Hill, Undine
AU  - Hill U
AD  - Erasmus Medical Center Stem Cell Institute, Department of Cell Biology, Erasmus 
      Medical Center, Rotterdam, the Netherlands.
FAU - Dzierzak, Elaine
AU  - Dzierzak E
AD  - Erasmus Medical Center Stem Cell Institute, Department of Cell Biology, Erasmus 
      Medical Center, Rotterdam, the Netherlands; Centre for Inflammation Research, 
      Queens Medical Research Institute, University of Edinburgh, Edinburgh, UK. 
      Electronic address: elaine.dzierzak@ed.ac.uk.
LA  - eng
GR  - R37 DK054077/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20171221
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
RN  - 0 (Antigens, Ly)
RN  - 0 (GATA2 Transcription Factor)
RN  - 0 (Gata2 protein, mouse)
RN  - 0 (Ly6a protein, mouse)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Ly/genetics/metabolism
MH  - *Cell Differentiation
MH  - *GATA2 Transcription Factor/genetics/metabolism
MH  - *Genes, Reporter
MH  - Hematopoietic Stem Cells/cytology/*metabolism
MH  - Membrane Proteins/genetics/metabolism
MH  - Mice
MH  - Mouse Embryonic Stem Cells/cytology/*metabolism
PMC - PMC5768964
OTO - NOTNLM
OT  - AGM
OT  - EMP
OT  - Gata2
OT  - Gata2Venus
OT  - Ly6a(SCA1)
OT  - Ly6a(SCA1)GFP
OT  - double reporter ESC
OT  - embryonic stem cells
OT  - hematopoiesis
OT  - hematopoietic differentiation
EDAT- 2017/12/26 06:00
MHDA- 2018/12/27 06:00
PMCR- 2017/12/21
CRDT- 2017/12/26 06:00
PHST- 2016/12/18 00:00 [received]
PHST- 2017/11/24 00:00 [revised]
PHST- 2017/11/27 00:00 [accepted]
PHST- 2017/12/26 06:00 [pubmed]
PHST- 2018/12/27 06:00 [medline]
PHST- 2017/12/26 06:00 [entrez]
PHST- 2017/12/21 00:00 [pmc-release]
AID - S2213-6711(17)30526-X [pii]
AID - 10.1016/j.stemcr.2017.11.018 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2018 Jan 9;10(1):151-165. doi: 10.1016/j.stemcr.2017.11.018. 
      Epub 2017 Dec 21.