PMID- 29278964
OWN - NLM
STAT- MEDLINE
DCOM- 20190523
LR  - 20190523
IS  - 1476-4954 (Electronic)
IS  - 1476-4954 (Linking)
VI  - 32
IP  - 10
DP  - 2019 May
TI  - Mild hypothermia protects neurons against oxygen glucose deprivation via poly 
      (ADP-ribose) signaling.
PG  - 1633-1639
LID - 10.1080/14767058.2017.1413548 [doi]
AB  - OBJECTIVE: Hypothermia is a neuroprotective mechanism that has been validated for 
      use in alleviating neonatal hypoxic-ischemic (HI) brain injury. Nevertheless, it 
      is unclear whether poly (ADP-ribose) (PAR) signaling is involved in 
      hypothermia-induced neuroprotection. In this study, we investigated whether mild 
      hypothermia rescues oxygen glucose deprivation (OGD)-induced cell death by 
      modifying PAR-relative protein expression, such as AIF, PARP-1, and PAR polymer, 
      in primary-cultured hippocampal neurons. METHODS: We analyzed neuronal morphology 
      and related protein expression of PAR signaling after OGD followed by mild 
      hypothermia in primary-cultured newborn hippocampal neurons. RESULTS: Hypothermic 
      treatment resulted in improved neuronal viability and alleviated DNA damage. 
      Results from the protein assay showed that hypothermia attenuated nuclear 
      translocation of apoptosis-inducing factor (AIF), inhibited overactivation of 
      poly(ADP-ribose) polymerase-1 (PARP-1), and decreased production of PAR polymer 
      induced by PARP-1 activation after OGD. CONCLUSIONS: These results showed that 
      mild hypothermia partially protects immature hippocampal neurons against OGD 
      injury in part by interfering with the PAR signaling pathway.
FAU - Gao, Yubo
AU  - Gao Y
AD  - a Department of Anaesthesiology , General Hospital of Ningxia Medical University 
      , Yinchuan , China.
FAU - Wang, Zhihua
AU  - Wang Z
AD  - a Department of Anaesthesiology , General Hospital of Ningxia Medical University 
      , Yinchuan , China.
FAU - He, Weikun
AU  - He W
AD  - a Department of Anaesthesiology , General Hospital of Ningxia Medical University 
      , Yinchuan , China.
FAU - Ma, Wenjing
AU  - Ma W
AD  - a Department of Anaesthesiology , General Hospital of Ningxia Medical University 
      , Yinchuan , China.
FAU - Ni, Xinli
AU  - Ni X
AD  - a Department of Anaesthesiology , General Hospital of Ningxia Medical University 
      , Yinchuan , China.
LA  - eng
PT  - Journal Article
DEP - 20171226
PL  - England
TA  - J Matern Fetal Neonatal Med
JT  - The journal of maternal-fetal & neonatal medicine : the official journal of the 
      European Association of Perinatal Medicine, the Federation of Asia and Oceania 
      Perinatal Societies, the International Society of Perinatal Obstetricians
JID - 101136916
RN  - 0 (Apoptosis Inducing Factor)
RN  - EC 2.4.2.30 (PARP1 protein, human)
RN  - EC 2.4.2.30 (Poly (ADP-Ribose) Polymerase-1)
SB  - IM
MH  - Animals
MH  - Apoptosis Inducing Factor/metabolism
MH  - Blotting, Western
MH  - Hippocampus/*metabolism/pathology
MH  - Humans
MH  - Hypothermia
MH  - Hypoxia-Ischemia, Brain/*metabolism/*prevention & control
MH  - Neurons/*metabolism/pathology
MH  - Neuroprotection
MH  - Poly (ADP-Ribose) Polymerase-1/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Apoptosis-inducing factor
OT  - mild hypothermia
OT  - neuron
OT  - oxygen-glucose deprivation
OT  - poly(ADP-ribose)
EDAT- 2017/12/28 06:00
MHDA- 2019/05/24 06:00
CRDT- 2017/12/28 06:00
PHST- 2017/12/28 06:00 [pubmed]
PHST- 2019/05/24 06:00 [medline]
PHST- 2017/12/28 06:00 [entrez]
AID - 10.1080/14767058.2017.1413548 [doi]
PST - ppublish
SO  - J Matern Fetal Neonatal Med. 2019 May;32(10):1633-1639. doi: 
      10.1080/14767058.2017.1413548. Epub 2017 Dec 26.