PMID- 29279046
OWN - NLM
STAT- MEDLINE
DCOM- 20190121
LR  - 20190121
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 24
IP  - 13-14
DP  - 2018 Jul
TI  - Human Wharton's Jelly Cells Activate Degenerative Nucleus Pulposus Cells In 
      Vitro.
PG  - 1035-1043
LID - 10.1089/ten.TEA.2017.0340 [doi]
AB  - To investigate the interaction between human Wharton's jelly cells (WJCs) and 
      degenerative nucleus pulposus cells (NPCs), human WJCs were cocultured with 
      degenerative NPCs with or without direct cell-cell contact. WJCs were isolated 
      from the human umbilical cord and degenerative NPCs were isolated from the 
      surgically obtained degenerative intervertebral disc tissue. The isolated WJCs 
      positively expressed CD73, CD105, CD90, CD29, CD166, and human leukocyte antigen 
      (HLA)-ABC, but negatively expressed CD34, CD45, and HLA-DR. After coculturing 
      with three different WJCs:NPCs ratios for 7 days, the real-time polymerase chain 
      reaction showed that the relative gene expression of nucleus pulposus (NP)-marker 
      genes [aggrecan, type II collagen, and SRY-type HMG box-9 (SOX-9)] was 
      significantly upgraded in all coculture groups (all p < 0.05 compared with 
      control groups). Coculture either with or without cell-cell contact significantly 
      activated the expression of NP-maker genes than controls, but coculture with 
      cell-cell contact yielded a higher gene expression than coculture without 
      cell-cell contact. In coculturing with cell-cell contact and WJCs:NPCs of 25:75, 
      the relative gene expression of aggrecan, type II collagen, SOX-9 for WJCs 
      yielded the highest increase by 721-, 1507-, and 1463-folds, respectively (all 
      p < 0.05 compared with WJCs control). In contrast, the highest relative gene 
      expression of aggrecan, type II collagen, SOX-9 for NPCs was 112-, 84-, and 
      109-folds, respectively, in coculture with cell-cell contact and in WJCs:NPCs of 
      75:25 (all p < 0.05 compared with NPCs control). In conclusion, the data 
      indicated that coculturing human WJCs with degenerative NPCs induced the NP-like 
      cell differentiation of WJCs and restored the biological status of degenerative 
      NPCs and coculture WJCs and NPCs with direct cell-cell contact yielded more 
      favorable gene expressions.
FAU - Han, Zhihua
AU  - Han Z
AD  - 1 Department of Orthopaedic Surgery, Navy General Hospital of PLA , Beijing, 
      China .
AD  - 2 Experimental Trauma and Orthopedic Surgery, Frankfurt Initiative for 
      Regenerative Medicine, J.W. Goethe-University , Frankfurt, Germany .
FAU - Zhang, Yan
AU  - Zhang Y
AD  - 1 Department of Orthopaedic Surgery, Navy General Hospital of PLA , Beijing, 
      China .
AD  - 3 Department of VIP Neurology, Navy General Hospital of PLA , Beijing, China .
FAU - Gao, Liang
AU  - Gao L
AD  - 4 Center of Experimental Orthopaedics, Saarland University Medical Center , 
      Homburg, Germany .
FAU - Jiang, Shujun
AU  - Jiang S
AD  - 3 Department of VIP Neurology, Navy General Hospital of PLA , Beijing, China .
FAU - Ruan, Dike
AU  - Ruan D
AD  - 1 Department of Orthopaedic Surgery, Navy General Hospital of PLA , Beijing, 
      China .
LA  - eng
PT  - Journal Article
DEP - 20180221
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Aggrecans)
RN  - 0 (Collagen Type II)
SB  - IM
MH  - Adult
MH  - Aggrecans/genetics/metabolism
MH  - Cell Shape
MH  - Coculture Techniques
MH  - Collagen Type II/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Intervertebral Disc Degeneration/diagnostic imaging/*pathology
MH  - Lumbar Vertebrae/diagnostic imaging/pathology
MH  - Magnetic Resonance Imaging
MH  - Nucleus Pulposus/*pathology
MH  - Spondylosis/diagnostic imaging/pathology
MH  - Staining and Labeling
MH  - Wharton Jelly/*cytology
OTO - NOTNLM
OT  - Wharton's jelly cells
OT  - coculture
OT  - intervertebral disc degeneration
OT  - nucleus pulposus cells
EDAT- 2017/12/28 06:00
MHDA- 2019/01/22 06:00
CRDT- 2017/12/28 06:00
PHST- 2017/12/28 06:00 [pubmed]
PHST- 2019/01/22 06:00 [medline]
PHST- 2017/12/28 06:00 [entrez]
AID - 10.1089/ten.TEA.2017.0340 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2018 Jul;24(13-14):1035-1043. doi: 10.1089/ten.TEA.2017.0340. 
      Epub 2018 Feb 21.