PMID- 29280053
OWN - NLM
STAT- MEDLINE
DCOM- 20180827
LR  - 20191126
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 64
IP  - 2
DP  - 2018 Feb
TI  - CD200Fc Attenuates Retinal Glial Responses and RGCs Apoptosis After Optic Nerve 
      Crush by Modulating CD200/CD200R1 Interaction.
PG  - 200-210
LID - 10.1007/s12031-017-1020-z [doi]
AB  - To explore the hypothesis that CD200Fc, a CD200R1 agonist with anti-inflammatory 
      properties, will inhibit retinal glial cells hyperactivation and retinal ganglion 
      cells (RGCs) apoptosis after optic nerve injury. CD200Fc was immediately 
      administered after optic nerve crush (ONC) once by intravitreal injection. Rats 
      were euthanized at 5 days after ONC. The density of RGCs was counted by 
      immunostaining of retina flat mounts for Brn3a. TUNEL assay, immunoblotting 
      analysis of ionized calcium-binding adapter molecule 1(iba1) (microglia marker) 
      and glial fibrillary acidic protein (GFAP) (astrocytes and Muller cells marker), 
      RT-PCR analysis of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase 
      (iNOS), monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-alpha (TNF-alpha), 
      interleukin (IL)-8 and IL-10, ELISA measure protein levels of inflammatory 
      cytokines and western blot analysis of CD200 and CD200R1 were evaluated. CD200Fc 
      treatment suppressed ONC-induced RGCs loss through inhibition of RGCs apoptosis. 
      Additionally, expression of glial cells activation markers GFAP and iba1 and 
      production of pro-inflammatory cytokines (COX-2, iNOS, MCP-1, TNF-alpha, IL-8) were 
      decreased in CD200Fc treated animals after ONC. Meanwhile, anti-inflammatory 
      cytokine IL-10 was increased by CD200Fc treatment in ONC-induced rat retina. 
      Finally, we found that CD200Fc significantly inhibited ONC-induced increased in 
      expression of CD200 and raised the already high basal CD200R1 expression in the 
      rat retina after ONC. Our results demonstrated that the anti-inflammatory effects 
      of CD200Fc in ONC rats model through inhibited the activation of retinal glial 
      cells via the interaction between CD200 and CD200R1, and the neuroprotective 
      effects of CD200Fc on RGCs thought inhibited its apoptosis.
FAU - Huang, Rong
AU  - Huang R
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, 430060, 
      Hubei, People's Republic of China.
FAU - Lan, Qianqian
AU  - Lan Q
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Chen, Lifei
AU  - Chen L
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Zhong, Haibin
AU  - Zhong H
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Cui, Ling
AU  - Cui L
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Jiang, Li
AU  - Jiang L
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Huang, Hui
AU  - Huang H
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Li, Li
AU  - Li L
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Zeng, Siming
AU  - Zeng S
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China.
FAU - Li, Min
AU  - Li M
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China. eyeminli@163.com.
FAU - Zhao, Xin
AU  - Zhao X
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China. eyexinz@163.com.
FAU - Xu, Fan
AU  - Xu F
AUID- ORCID: 0000-0002-7464-2749
AD  - Department of Ophthalmology, People's Hospital of Guangxi Zhuang Autonomous 
      Region, Nanning, 530021, Guangxi, People's Republic of China. eyefanxu@163.com.
LA  - eng
GR  - 81660168/National Natural Science Foundation of China/
GR  - 81560166/National Natural Science Foundation of China/
GR  - 81460087/National Natural Science Foundation of China/
GR  - 81760172/National Natural Science Foundation of China/
GR  - 81760178/National Natural Science Foundation of China/
GR  - 81660161/National Natural Science Foundation of China (CN)/
GR  - No. 2012GXNSFAA276039/Natural Science Foundation of Guangxi Zhuang Autonomous 
      Region/
GR  - No. 2011GXNSFA018228/Natural Science Foundation of Guangxi Zhuang Autonomous 
      Region/
PT  - Journal Article
DEP - 20171226
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Antigens, CD)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Cd200 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (RGS Proteins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - UQ4V77A8VA (antigens, CD200)
SB  - IM
MH  - Animals
MH  - Antigens, CD/administration & dosage/pharmacology/*therapeutic use
MH  - *Apoptosis
MH  - Chemokine CCL2/metabolism
MH  - Cyclooxygenase 2/metabolism
MH  - Interleukin-8/metabolism
MH  - Intravitreal Injections
MH  - Male
MH  - Neuroglia/*drug effects/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Optic Nerve Injuries/*drug therapy
MH  - Protein Binding
MH  - RGS Proteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Immunologic/agonists/*metabolism
MH  - Retina/cytology/drug effects/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - CD200/CD200R interaction
OT  - CD200Fc
OT  - Inflammatory responses
OT  - Optic nerve crush
OT  - Retinal ganglion cells
OT  - Retinal glial cells
EDAT- 2017/12/28 06:00
MHDA- 2018/08/28 06:00
CRDT- 2017/12/28 06:00
PHST- 2017/10/12 00:00 [received]
PHST- 2017/12/15 00:00 [accepted]
PHST- 2017/12/28 06:00 [pubmed]
PHST- 2018/08/28 06:00 [medline]
PHST- 2017/12/28 06:00 [entrez]
AID - 10.1007/s12031-017-1020-z [pii]
AID - 10.1007/s12031-017-1020-z [doi]
PST - ppublish
SO  - J Mol Neurosci. 2018 Feb;64(2):200-210. doi: 10.1007/s12031-017-1020-z. Epub 2017 
      Dec 26.