PMID- 2928302
OWN - NLM
STAT- MEDLINE
DCOM- 19890428
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 86
IP  - 6
DP  - 1989 Mar
TI  - Reconstitution of Golgi vesicle CMP-sialic acid and adenosine 3'-phosphate 
      5'-phosphosulfate transport into proteoliposomes.
PG  - 1786-90
AB  - We have previously shown that Golgi apparatus vesicles transport nucleotide 
      sugars and nucleotide sulfate into their lumen. These transport activities are 
      organelle and substrate specific and are characterized by apparent Km for 
      nucleotide derivatives in the low micromolar range. As part of our goal of 
      purifying and characterizing the above transport proteins, we have reconstituted 
      a protein extract from rat liver Golgi membranes into phosphatidylcholine 
      liposomes. The resulting proteoliposomes transport CMP-N-acetylneuraminic acid 
      (CMP-AcNeu) and adenosine 3'-phosphate 5'-phosphosulfate with very similar 
      affinity and inhibition characteristics as intact Golgi vesicles. Sialic acid and 
      sodium sulfate, which are transported only very slowly into the lumen of Golgi 
      vesicles, are transported at low rates by the reconstituted proteoliposomes. 
      Neither rough endoplasmic reticulum-derived vesicles nor proteoliposomes made 
      from proteins of the rough endoplasmic reticulum transport CMP-AcNeu. The above 
      results demonstrate that this reconstituted system can be used for further 
      purification and characterization of nucleotide sugar and nucleotide sulfate 
      translocator proteins. This approach should also be useful to study membrane 
      transport proteins of lysosomes and endosomes.
FAU - Milla, M E
AU  - Milla ME
AD  - Department of Biochemistry, University of Massachusetts Medical Center, Worcester 
      01655.
FAU - Hirschberg, C B
AU  - Hirschberg CB
LA  - eng
GR  - GM 30365/GM/NIGMS NIH HHS/United States
GR  - GM 34396/GM/NIGMS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Adenine Nucleotides)
RN  - 0 (Liposomes)
RN  - 0 (Nucleotides)
RN  - 0 (Sialic Acids)
RN  - 27816-59-7 (4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid)
RN  - 3063-71-6 (Cytidine Monophosphate N-Acetylneuraminic Acid)
RN  - 482-67-7 (Phosphoadenosine Phosphosulfate)
RN  - F469818O25 (Cytidine Monophosphate)
RN  - Q1O6DSW23R (4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid)
SB  - IM
MH  - 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
MH  - 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/analogs & 
      derivatives/pharmacology
MH  - Adenine Nucleotides/*metabolism
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Cytidine Monophosphate/pharmacology
MH  - Cytidine Monophosphate N-Acetylneuraminic Acid/*metabolism
MH  - Endoplasmic Reticulum/metabolism
MH  - Freezing
MH  - Golgi Apparatus/*ultrastructure
MH  - Hot Temperature
MH  - Intracellular Membranes/*metabolism
MH  - Kinetics
MH  - Liposomes/*metabolism
MH  - Liver/*ultrastructure
MH  - Nucleotides/pharmacology
MH  - Phosphoadenosine Phosphosulfate/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sialic Acids/*metabolism
PMC - PMC286789
EDAT- 1989/03/01 00:00
MHDA- 1989/03/01 00:01
PMCR- 1989/09/01
CRDT- 1989/03/01 00:00
PHST- 1989/03/01 00:00 [pubmed]
PHST- 1989/03/01 00:01 [medline]
PHST- 1989/03/01 00:00 [entrez]
PHST- 1989/09/01 00:00 [pmc-release]
AID - 10.1073/pnas.86.6.1786 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1989 Mar;86(6):1786-90. doi: 10.1073/pnas.86.6.1786.