PMID- 29284210
OWN - NLM
STAT- MEDLINE
DCOM- 20180918
LR  - 20181113
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 19
IP  - 3
DP  - 2018 May 31
TI  - Genomic characterization and pathogenic study of two porcine reproductive and 
      respiratory syndrome viruses with different virulence in Fujian, China.
PG  - 339-349
LID - 10.4142/jvs.2018.19.3.339 [doi]
AB  - Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were 
      isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. 
      Inoculation experiments showed that FZ06A caused 100% morbidity and 60% 
      mortality, while FZ16A caused 100% morbidity without death. By using genomic 
      sequence and phylogenetic analyses, close relationships between a Chinese highly 
      pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on 
      the achieved results, multiple genomic variations in Nsp2, a unique 
      N-glycosylation site (N(3)(3)-->K(3)(3)), and a K151 amino acid (AA) substitution for 
      virulence in the GP5 of FZ16A were detected; except the 30 AA deletion in the 
      Nsp2-coding region. Inoculation experiments were conducted and weaker virulence 
      of FZ16A than FZ06A was observed. Based on our results, a 30 AA deletion in the 
      Nsp2-coding region is an unreliable genomic indicator of a high virulence PRRSV 
      strain. The Nsp2 and GP5 differences, in addition to the virulence difference 
      between these two highly pathogenic PRRSV strains, have the potential to be used 
      to establish a basis for further study of PRRSV virulence determinants and to 
      provide data useful in the development of vaccines against this economically 
      devastating disease.
FAU - Sui, Xiukun
AU  - Sui X
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
AD  - Molecular and Cellular Biology, Gembloux Agro-Bio Tech University of Liege, 5030 
      Gembloux, Belgium.
FAU - Xin, Ting
AU  - Xin T
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Guo, Xiaoyu
AU  - Guo X
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Jia, Hong
AU  - Jia H
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Li, Ming
AU  - Li M
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Gao, Xintao
AU  - Gao X
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Wu, Jing
AU  - Wu J
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
AD  - Molecular and Cellular Biology, Gembloux Agro-Bio Tech University of Liege, 5030 
      Gembloux, Belgium.
FAU - Jiang, Yitong
AU  - Jiang Y
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Willems, L
AU  - Willems L
AD  - Molecular and Cellular Biology, Gembloux Agro-Bio Tech University of Liege, 5030 
      Gembloux, Belgium.
FAU - Zhu, Hongfei
AU  - Zhu H
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
FAU - Hou, Shaohua
AU  - Hou S
AD  - Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 
      100193, China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
SB  - IM
MH  - Animals
MH  - China
MH  - *Genome, Viral
MH  - *Immunity, Humoral
MH  - Phylogeny
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*pathogenicity
MH  - Sequence Alignment
MH  - Swine/*immunology
MH  - Virulence
PMC - PMC5974515
OTO - NOTNLM
OT  - genomics
OT  - phylogeny
OT  - porcine respiratory and reproductive syndrome virus
OT  - virulence
COIS- Conflict of Interest: The authors declare no conflicts of interest.
EDAT- 2017/12/30 06:00
MHDA- 2018/09/19 06:00
PMCR- 2018/05/01
CRDT- 2017/12/29 06:00
PHST- 2017/08/11 00:00 [received]
PHST- 2017/10/25 00:00 [revised]
PHST- 2017/12/26 00:00 [accepted]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2018/09/19 06:00 [medline]
PHST- 2017/12/29 06:00 [entrez]
PHST- 2018/05/01 00:00 [pmc-release]
AID - jvs.18007 [pii]
AID - 10.4142/jvs.2018.19.3.339 [doi]
PST - ppublish
SO  - J Vet Sci. 2018 May 31;19(3):339-349. doi: 10.4142/jvs.2018.19.3.339.